DNA recombinase Rad51 is regulated with UVinduced DNA damage and the DNA mismatch repair inhibitor CdCl₂ in HC11 cells

Abstract

Homologous recombination is a fundamental process that occurs in all species, from bacteria to humans (Vispé et al., 1998). Rad51 and Dmc1, the central homologous pairing and strand exchange proteins, are the main recombinases in the homologous recombination pathway (Hinch et al., 2020). Dmc1 is known to be expressed only in meiosis, whereas Rad51 has been reported to be expressed in both meiosis and non-meiosis (Brown and Bishop, 2014). In order to confirm whether Rad51 and HR are related, various studies have been carried out to induce artificial double strand breaks (DSBs) with UV irradiation and ionizing-radiation within cells (Elvers et al., 2011; Julien et al., 2013). It has been reported that the expression of Rad51 is increased during the S phase when irradiated with UV in human cells (Tashiro et al., 2000).The DNA repair pathway HR is also associated with mismatch repair (MMR) (Spies and Fishel, 2015). MMR is a highly preserved post-replication repair system that rec-