DNA quadruplexes as molecular scaffolds for controlled assembly of fluorogens with aggregation-induced emission

Zhu, Longyi; Zhou, Jun; Xu, Guisheng; Li, Conggang; Ling, Pinghua; Liu, Bin; Ju, Huangxian; Lei, Jianping

doi:10.1039/c8sc00001h

Cited by 42 publications

(23 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lei et al made a molecular scaffold based on a tetrapod DNA quadruplex (TP-G4) for self-assembly and precise control of the AIE-effect-based fluorescence signal transduction. [118] As shown in Figure 9D, the scaffold TP-G4 was formed by four O1ʹ-6G strands that can provide a confined space for the regulation of the AIE effect. A DNA-AIEgen conjugate, O1-DTPE, was synthesized by "copper-free" click coupling, which was water-soluble and exhibited low fluorescence in water.…”

Section: Dna Biosensors Based On the Self-assembly Of Dna Amphiphilesmentioning

confidence: 99%

Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids

et al. 2020

View full text Add to dashboard Cite

The comprehensive understanding and proper use of supramolecular interactions have become critical for the development of functional materials, and so is the biomedical application of nucleic acids (NAs). Relatively rare attention has been paid to hydrophobic interaction compared with hydrogen bonding and electrostatic interaction of NAs. However, hydrophobic interaction shows some unique properties, such as high tunability for application interest, minimal effect on NA functionality, and sensitivity to external stimuli. Therefore, the widespread use of hydrophobic interaction has promoted the evolution of NA-based biomaterials in higher-order self-assembly, drug/gene-delivery systems, and stimuli-responsive systems. Herein, the recent progress of NA-based biomaterials whose fabrications or properties are highly determined by hydrophobic interactions is summarized. 1) The hydrophobic interaction of NA itself comes from the accumulation of base-stacking forces, by which the NAs with certain base compositions and chain lengths show properties similar to thermal-responsive polymers. 2) In conjugation with hydrophobic molecules, NA amphiphiles show interesting self-assembly structures with unique properties in many new biosensing and therapeutic strategies. 3) The working-mechanisms of some NA-based complex materials are also dependent on hydrophobic interactions. Moreover, in recent attempts, NA amphiphiles have been applied in organizing macroscopic self-assembly of DNA origami and controlling the cell-cell interactions.

show abstract

Section: Dna Biosensors Based On the Self-assembly Of Dna Amphiphilesmentioning

confidence: 99%

Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Lei et al [31] constructed aq uadruped DNA quadruplex (TP-G4) behaving as am olecular bracket for accurate tuning of the fluorescencee mission of an oligonucleotide-grafted AIEgen (Oligo-AIEgen) ( Figure 4A). The Oligo-AIEgen was constructed through covalently conjugatingt he AIEgen to the 3'-end of the oligonucleotide by meanso fadibenzylcyclooctyne-mediated coupled reaction.…”

Section: Constant Dna Lengthmentioning

confidence: 99%

“…Lei et al . constructed a quadruped DNA quadruplex (TP‐G4) behaving as a molecular bracket for accurate tuning of the fluorescence emission of an oligonucleotide‐grafted AIEgen (Oligo‐AIEgen) (Figure A).…”

Section: Conformational Transitions Of Dnamentioning

confidence: 99%

AIEgens/Nucleic Acid Nanostructures for Bioanalytical Applications

Wang

Huang

et al. 2019

Chemistry An Asian Journal

View full text Add to dashboard Cite

DNA occupies significant roles in life processes, which include encoding the sequences of proteins and accurately transferring genetic information from generation to generation. Recent discoveries have demonstrated that a variety of biological functions are correlated with DNA′s conformational transitions. The non‐B form has attained great attention among the diverse forms of DNA over the past several years. The main reason for this is that a large number of studies have shown that the non‐B form of DNA is associated with gross deletions, inversions, duplications, translocations as well as simple repeating sequences, which therefore causes human diseases. Consequently, the conformational transition of DNA between the B‐form and the non‐B form is important for biology. Conventional fluorescence probes based on the conformational transitions of DNA usually need a fluorophore and a quencher group, which suffers from the complex design of the structure and tedious synthetic procedures. Moreover, conventional fluorescence probes are subject to the aggregation‐caused quenching (ACQ) effect, which limits their application toward imaging and analyte detection. Fluorogens exhibiting aggregation‐induced emission (AIE) have attracted tremendous attention over the past decade. By taking advantage of this unique behavior, plenty of fluorescent switch‐on probes without the incorporation of fluorescent quenchers/fluorophore pairs have been widely developed as biosensors for imaging a variety of analytes. Herein, the recent progress in bioanalytical applications on the basis of aggregation‐induced emission luminogens (AIEgens)/nucleic acid nanostructures are presented and discussed.

show abstract

“…As a new type of fluorescent material, aggregation-induced emission luminogens (AIEgens) shows weak or negligible emission in dilute solution, but it emits highly in aggregate or solid state (Vendrell et al, 2012;Guan et al, 2017;Chen Y et al, 2018;Liu and Tang, 2019). 1,1,2,2-tetrakis[4-(2-bromo-ethoxy) phenyl]ethene (TTAPE) is a fully water-soluble AIEgens with positive electricity, and can emit a blue fluorescence signal after binding with DNA via electrostatic interaction (Lou et al, 2015;Daher et al, 2016;Zhu et al, 2018). However, If TTAPE combined with recombinase polymerase amplification (RPA) to directly detect, it is easy to cause false positive results.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens

Jiao

Yang

Long³

et al. 2022

Front. Chem.

View full text Add to dashboard Cite

Here, we developed a rapid, visual and double-checked Logic Gate detection platform for detection of pathogenic microorganisms by aggregation-induced emission luminogens (AIEgens) in combination with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated (Cas). DNA light-up AIEgens (1,1,2,2-tetrakis[4-(2-bromo-ethoxy) phenyl]ethene, TTAPE) was non-emissive but the emission was turned on in the presence of large amount of DNA produced by recombinase polymerase amplification (RPA). When CRISPR/Cas12a was added, all long-stranded DNA were cut leading to the emission quenched. Thus, a method that can directly observe the emission changes with the naked eye has been successfully constructed. The detection is speedy within only 20 min, and has strong specificity to the target. The result can be judged by Logic Gate. Only when the output signal is (1,0), does it represent the presence of pathogenic microorganisms in the test object. Finally, the method was applied to the detect pathogenic microorganisms in environmental water samples, which proved that this method has high selectivity, specificity and applicability for the detection of pathogenic microorganisms in environmental water samples.

show abstract

DNA quadruplexes as molecular scaffolds for controlled assembly of fluorogens with aggregation-induced emission

Abstract: Tetrapod DNA quadruplexes were designed for assembly and precise modulation of light emission of an oligonucleotide-grafted fluorogen with aggregation-induced emission.

Cited by 42 publications

References 50 publications

Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids

Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids

AIEgens/Nucleic Acid Nanostructures for Bioanalytical Applications

CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens

Contact Info

Product

Resources

About