DNA profiling from blood traces present on clothing’s and detected by Benzidine test in forensic cases

Abstract

Blood is the most common form of biological evidences that encounters in several types of crime i.e. murder, sexual assault/ rape, hit and run cases, tool marks or many types of heinous crime. But in most of the cases, blood on the clothing's or any nearby surfaces are washed off by the perpetrator/accused person in order to destroy the trail of evidences left behind him/her. The stains/doubtful spots are always examined for the presence of blood with the help of presumptive blood tests, such as benzidine test or phenolphthalein test. In this present study, 10 such samples of benzidine-tested cloth and a few blood droplets were lifted from the various surfaces. For DNA isolation, quantification and profiling; un-tested portion of same samples and their respective reference blood samples were collected. A comparative study was conducted between the DNA quantities of benzidine tested portion of samples along with DNA quantity of untested portion of the same samples. Now, all the DNA profiles were compared with the DNA profile obtained from their respective reference blood samples. As a resultant of this study, it was found that DNA profile was not affected by the benzidine test. However, minutiae difference in DNA quantity was observed.

Molecular Markers for Human Sex Determination in Forensic Genetics Analysis

“…Blood samples are the ideal form for biological evidences that are collected from different kinds of crimes i.e. murder, rape-sexual homicide, hit and run, road accident cases, tool marks, or other types of a heinous crime [4]. Genomic DNA isolation from whole blood samples afford high quantity and quality DNA compared to any other biological source [5,6].…”