DNA profiles from clothing fibers using direct PCR

Blackie, Renée; Taylor, Duncan; Linacre, Adrian

doi:10.1007/s12024-016-9784-y

Cited by 24 publications

(11 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Personal items and clothing are often collected as evidentiary or reference material. It is usually assumed that these will provide the profile of the regular user/wearer, which they usually do; however, they often also contain DNA from other sources [64,82,104,123,137,[160][161][162][163][164]. For example:…”

Section: Prevalence and Origin Of Non-self Dna On Personal Itemsmentioning

confidence: 99%

DNA transfer in forensic science: A review

Oorschot

Szkuta

Meakin³

et al. 2019

Forensic Science International: Genetics

215

130

View full text Add to dashboard Cite

Understanding the variables impacting DNA transfer, persistence, prevalence and recovery (DNA-TPPR) has become increasingly relevant in investigations of criminal activities to provide opinion on how the DNA of a person of interest became present within the sample collected. This review considers our current knowledge regarding DNA-TPPR to assist casework investigations of criminal activities. There is a growing amount of information available on DNA-TPPR to inform the relative probabilities of the evidence given alternative scenarios relating to the presence or absence of DNA from a specific person in a collected sample of interest. This information should be used where relevant. However, far more research is still required to better understand the variables impacting DNA-TPPR and to generate more accurate probability estimates of generating particular types of profiles in more casework relevant situations. This review explores means of achieving this. It also notes the need for all those interacting with an item of interest to have an awareness of DNA transfer possibilities post criminal activity, to limit the risk of contamination or loss of DNA. Appropriately trained forensic practitioners are best placed to provide opinion and guidance on the interpretation of profiles at the activity level. However, those requested to provide expert opinion on DNA-related activity level issues are often insufficiently trained to do so. We advocate recognition of DNA activity associated expertise to be distinct from expertise associated with the identification of individuals. This is to be supported by dedicated training, competency testing, authorisation, and regular fit for purpose proficiency testing. The possibilities for experts to report on activity-related issues will increase as our knowledge increases through further research, access to relevant data is enhanced, and tools to assist interpretations are better exploited. Improvement opportunities will be achieved sooner, if more laboratories and agencies accept the need to invest in these aspects as well as the training of practitioners.

show abstract

Section: Prevalence and Origin Of Non-self Dna On Personal Itemsmentioning

confidence: 99%

DNA transfer in forensic science: A review

Oorschot

Szkuta

Meakin³

et al. 2019

Forensic Science International: Genetics

215

130

View full text Add to dashboard Cite

show abstract

“…Though the authors observed mixed DNA profiles, the major informative ones always matched the donor. The method has also been used to generate full genetic profiles from single hair follicles [58], fingernails clippings [101], clothing fibres [106] and touch DNA from various sources [107].…”

Section: Direct Pcrmentioning

confidence: 99%

Forensic touch DNA recovery from metal surfaces – A review

2020

View full text Add to dashboard Cite

show abstract

“…Direct amplification kits are tolerant of higher DNA inputs and inhibitors [26,27], and in some cases, direct PCR has been shown to be more efficient than traditional extraction-based processing [28,29]. Successful direct amplification for STR typing has been demonstrated with DNA present on clothing fibers [30], fingernails [31], hair follicles [32], and biological samples like preserved tissue and bloodstains [27,29,33]. Direct amplification has also been utilized with massively parallel sequencing for human identification [34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Collection and storage of DVI samples with microFLOQ® Direct swabs for direct amplification

Loockerman

Miller

Ramsey

et al. 2021

Forensic Science International: Genetics

View full text Add to dashboard Cite

The rapid identification of decomposing human remains is a crucial component of disaster victim identification (DVI), often occurring in remote areas without access to laboratory or storage facilities. Due to the ease of collection and amenability to storage in harsh conditions, swabs may be used to collect DNA from decomposing remains as an alternative to sampling tissue or bone. Direct amplification could further streamline the process and reduce costs. This study investigated the efficacy of direct amplification of DVI samples using microFLOQ® Direct swabs and the QIAGEN Investigator QS GO! Kit. A comparison of performance between direct amplification and traditional methods was made to assess whether direct amplification offered an improvement to traditional methods. DNA was collected by swabbing the muscle of a decomposing human cadaver using three swab types (ADS Genetics 4N6FLOQSwabs®, NADS Genetics 4N6FLOQSwabs®, and the microFLOQ® Direct swab). Traditional swabs (4N6FLOQSwabs®) were extracted and quantified, while a direct amplification strategy was used with the microFLOQ® Direct swabs coupled with the Investigator 24Plex GO! Kit. Processing of the microFLOQ® Direct swabs were optimized and a hybrid strategy that used 4N6FLOQSwabs® to collect and store DNA before swabbing or "subsampling" the 4N6FLOQSwabs® for processing with microFLOQ® Direct swabs was developed. This hybrid strategy allowed for rapid processing without the consumption of the original sample. Traditional and direct PCR methods were comparable up to day 10 of decomposition depending on the sample location and for up to 3 months of storage at room temperature. This research indicated that microFLOQ® Direct swabs in conjunction with the Investigator 24Plex GO! Kit can be used to facilitate rapid direct processing of DNA from decomposing human remains.

show abstract

DNA profiles from clothing fibers using direct PCR

Cited by 24 publications

References 25 publications

DNA transfer in forensic science: A review

DNA transfer in forensic science: A review

Forensic touch DNA recovery from metal surfaces – A review

Collection and storage of DVI samples with microFLOQ® Direct swabs for direct amplification

Contact Info

Product

Resources

About