DNA probes in the diagnosis of genetic and infectious diseases

Kingsbury, David T.

doi:10.1016/0167-7799(87)90029-1

Cited by 12 publications

(3 citation statements)

References 11 publications

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“…fungi (Crowley and Oliver, 1987;Manicom et al, 1987;Rollo et al, 1987) and protozoa (Kukla et al, 1987). It is also used routinely in medical diagnostic programmes (Hejtmancik etal.. 1986;Kingsbury, 1987).…”

Section: Nucleic Acid Hybridization Methodsmentioning

confidence: 99%

“…Hybridization applications include detection of various viruses and viroids (Bar-Joseph and Rosner, 1984;Hull, 1984;Diener and Owens, 1985;Lakshman et al, 1986;Haber, Polston and Bird, 1987;Kingsbury. 1987;Mills, 1987;Varveri, Ravelonandro and Dunez, 1987), fastidious prokaryotes (Borkhsenius, et al, 1987;Kirkpatrick et al.…”

Section: Nucleic Acid Hybridization Methodsmentioning

confidence: 99%

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New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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Section: Nucleic Acid Hybridization Methodsmentioning

confidence: 99%

Section: Nucleic Acid Hybridization Methodsmentioning

confidence: 99%

New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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“…[40]), where they have found application in the diagnosis of viral infections by detection of viral sequences in specimens (for a review see Ref. [222]), the detection of other infections,12221 of genetic diseases, [40. 2221 and of neoplastic diseases,[401 and the identification of persons. [401 Under optimal assay conditions these assays are sensitive enough to detect one nucleobase exchange in a target sequence.…”

Section: The System Oligonucleotide-biotin-avidiwxxxmentioning

confidence: 99%

Chemically Modified Oligonucleotides as Probes and Inhibitors

Englisch

Gauss

1991

Angew. Chem. Int. Ed. Engl.

158

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Oligonucleotides bind specifically to single‐stranded nucleic acids to form a double helix if there is a complementary antiparallel nucleotide sequence. In addition, certain oligonucleotides bind specifically to a variety of proteins. Therefore, biological processes involving these nucleic acids or proteins can be modulated (normally inhibited) by addition of the respective oligonucleotides. The effects of these oligonucleotides and the fields of potential application can be broadened by the introduction of chemically modified nucleotides. For instance, replacing oligonucleotide phosphate groups by methylphosphonates results in the loss of one negative charge per nucleotide and the oligonucleotide becomes more lipophilic. An oligonucleotide carrying a reactive group can modify its binding partner. An oligonucleotide covalently linked to a dye can be localized in a biological specimen. Oligonucleotides attached to an enzyme can be detected in very small amounts since the enzyme can catalyze the formation of large amounts of the substance assayed (e.g., a fluorescent product). An important biochemical application is the detection and localization of a messenger RNA or its gene. Medical applications include the detection of bacterial or viral sequences. There is also great interest in inhibiting the translation of messenger RNA and the transcription and replication of DNA. So‐called antisense oligonucleotides are currently being used for the inhibition of protein biosynthesis and of reverse transcription of retroviruses.

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