DNA probes for Mycoplasma gallisepticum and Mycoplasma synoviae: Application in experimentally infected chickens

Experimental Coinfection of Chicken Anemia Virus and Mycoplasma gallisepticum Vaccine Strains in Broiler Chicks

“…At 23 days of age, all birds were euthanized, submitted to full necropsy, and the liver, spleen, thymus, and trachea were collected for histopathology and thymus and trachea for the investigation of CAV or MG by PCR. Considering that the infection by Mycoplasma gallisepticum strains may be evaluated in the trachea, especially during the acute stage of infection previous to the period needed for an antibody response (typically 21 days) to be detectable in all birds (Levisohn & Dykstra, 1987), the isolation or detection by molecular methods is most successful during the acute stage of infection (Hyman et al, 1989). In addition, the duration of the experiment was reduced to a minimum in view of the risk of field CAV challenge.…”

Genotypic Methods for Diagnosis of Mycoplasmal Infections in Humans, Animals, Plants and Cell Cultures

“…Sensitive detection methods for systematic testing are required to guarantee that animals and their progeny are free from these mycoplasmas. DNA probes for M. gallisepticum and M. synoviae were utilized in hybridization protocols because of their ability to detect new infection (1Chan et al, 1987;Santhaex al., 1987;Geary etal., 1988;Stipkovits etal., 1988;Hyman et al, 1989;Levisohn et a/., 1989). Geary and colleagues (1988) described a detection procedure with a biotinylated DNA probe which could be advantageous for safety reasons.…”

The use of a specific dna probe for detection ofMycoplasma gallisepticumin field outbreaks

“…The probe was shown to recognise a wide spectrum of M gallisepticum strains, but did not hybridise with DNA of M synoviae, other avian mycoplasmas and bacteria associated with the diseased trachea (Hyman et al, 1989). The probe detected as few as 10 5 MG organisms in culture, and revealed MG in trachéal swabs of live or necropsied chickens as early as 1 week after experimental infection or contact exposure.…”