DNA probe culture confirmation assay for identification of thermophilic Campylobacter species

Tenover, Fred C.; Carlson, L G; Barbagallo, S; Nachamkin, Irving

doi:10.1128/jcm.28.6.1284-1287.1990

Cited by 34 publications

(9 citation statements)

References 26 publications

(17 reference statements)

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“…Although some sequence variation was present in V5, the thermotolerant Campylobacter spp. could not be discriminated because the three species are closely related; previous reports described difficulties in discriminating among these species with biochemical tests (11) or DNA probes (9,14,19).…”

Section: Discussionmentioning

confidence: 99%

Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction

Giesendorf

Quint

Henkens

et al. 1992

Appl Environ Microbiol

143

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The polymerase chain reaction (PCR) after a short enrichment culture was used to detect Campylobacter spp. in chicken products. After the 16S rRNA gene sequence of Campylobacterjejuni was determined and compared with known sequences from other enterobacteria, a primer and probe combination was selected from the region before V3 and the variable regions V3 and V5. With this primer set and probe, 426-bp fragments from C. jejuni, Campylobacter coli, and Campylobacter lari could be amplified. The detection limit of the PCR was 12.5 CFU. Chicken samples inoculated with 25 CFU of Campylobacter spp. per g were PCR positive after an 18-h enrichment, which resulted in 500 CFU/ml of culture broth. This PCR-culture assay was compared with the

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Section: Discussionmentioning

confidence: 99%

Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction

Giesendorf

Quint

Henkens

et al. 1992

Appl Environ Microbiol

143

View full text Add to dashboard Cite

show abstract

“…Each of these systems involves nonradioactive methods for detection, facilitating their usage. Their efficacy has been evaluated (10,29,171,173), and the manufacturers' claims for their respective products have been confirmed. However, weak reactions with C. lari with the SNAP kit were observed, although this system was seen to react with C. hyointestinalis and C. upsaliensis isolates (173).…”

Section: Nucleic Acid Probesmentioning

confidence: 96%

Identification methods for campylobacters, helicobacters, and related organisms

1996

Clin Microbiol Rev

265

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The organisms which are referred to as campylobacteria are associated with a diverse range of diseases and habitats and are important from both clinical and economic perspectives. Accurate identification of these organisms is desirable for deciding upon appropriate therapeutic measures, and also for furthering our understanding of their pathology and epidemiology. However, the identification process is made difficult because of the complex and rapidly evolving taxonomy, fastidious nature, and biochemical inertness of these bacteria. These problems have resulted in a proliferation of phenotypic and genotypic methods for identifying members of this group. The purpose of this review is to summarize the problems associated with identifying campylobacteria, critically appraise the methods that have been used for this purpose, and discuss prospects for improvements in this field.

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“…Although these techniques in some cases provide definitive identification of campylobacters and allow new species to be characterized, most of the procedures are complex and most involve the use of radiolabeled probes, which is impractical for routine clinical use. The majority of these methods are also limited in their ability to discriminate between closely related thermophilic campylobacters (5,15).…”

mentioning

confidence: 99%

Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant

et al. 1997

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A PCR method for the rapid identification and discrimination of thermophilic Campylobacter jejuni and Campylobacter coli was developed by using a gene encoding a protein involved in siderophore transport (ceuE). A nucleotide sequence divergence of approximately 13% in the ceuE genes of C. jejuni and C. coli facilitated the design of two species-specific PCR primer sets. The specificity of the PCR amplification reactions was confirmed by using two nonradioactively labelled species-specific internal oligonucleotide hybridization probes for each of these species.

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DNA probe culture confirmation assay for identification of thermophilic Campylobacter species

Cited by 34 publications

References 26 publications

Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction

Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction

Identification methods for campylobacters, helicobacters, and related organisms

Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant

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