DNA prime–protein boost immunization in monkeys: efficacy of a novel construct containing functional domains ofPlasmodium cynomolgiCS and TRAP

Abstract

We report the efficacy of a bimodal immunization regimen that involved priming with naked DNA (multiple doses) followed by a booster with recombinant protein in rhesus monkeys with a chimeric construct containing the N-terminus of thrombospondin-related adhesive protein and the C-terminus of circumsporozoite protein of Plasmodium cynomolgi. The vaccinated animals developed high titer antibodies against the chimeric antigen, the two components of the hybrid and the native proteins of sporozoites. The peripheral blood mononuclear cells isolated from the vaccinated animals had significant in vitro T cell proliferation activity when stimulated with the recombinant chimeric protein. Furthermore, following challenge with 1000 P. cynomolgi sporozoites, the peak and total parasitemia were significantly lower in vaccinated animals than in the control animals.

Human Metapneumovirus Fusion Protein Vaccines That Are Immunogenic and Protective in Cotton Rats

“…The technique of DNA immunization against viruses has been studied extensively in animal and human studies (1,3,4,8,9,12,24,25,44,47). Ulmer et al first showed that mice could be protected against a lethal influenza virus challenge by immunization with a DNA plasmid containing an influenza virus gene (44).…”

Non-human primate malaria parasites: out of the forest and into the laboratory

“…Strain-specific immunity, a trait found both in human and rodent malaria (Ciuca et al 1934;Jarra and Brown, 1985), has been observed in P. cynomolgi (Wijayalath et al 2008(Wijayalath et al , 2012. Additionally, P. cynomolgi has also been used to test the efficacy of potential vaccination strategies (Millet et al 1992(Millet et al , 1995Barnwell et al 1999;Bhardwaj et al 2003;Dutta et al 2005).…”

Plasmodium fieldi: Observations on the Hackeri and ABI Strains in Macaca mulatta Monkeys and Mosquitoes

“…It has been used in different drug studies and in vaccine trials as well as in basic biologic investigations. [33][34][35][36][37] Plasmodium cynomolgi can be transmitted experimentally by many different species of mosquitoes, from both the Anopheles and Cellia subgenera. In contrast to P. knowlesi , this is an example of an excellent choice of a model for studies that relate to a human malaria parasite, such as P. vivax .…”