DNA Polymorphisms - Practical use

Werrett, David J.; Gill, Peter; Lygo, Joan E.; Fowler, Susan J.

doi:10.1007/978-3-642-73330-7_63

Cited by 6 publications

(3 citation statements)

References 19 publications

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“…A final microdialysis through a Millipore filter (0.05 gin) as described by Werrett et al [1], was carried out when indicated in the text after treatment with various chemical substances before and/ or after endonuclease restriction.…”

Section: Methodsmentioning

confidence: 99%

“…Aliquots of the digests (1 or 2 pl) were electrophoresed and inspected to assess the extent of digestion, Some of the undigested samples were dialyzed as described [1] or re-extracted, and then redigested as above. Aliquots of the digests (1 or 2 pl) were electrophoresed and inspected to assess the extent of digestion, Some of the undigested samples were dialyzed as described [1] or re-extracted, and then redigested as above.…”

Section: Dna Restriction Electrophoresis Blotting and Characterizatmentioning

confidence: 99%

“…Low yields and partial restriction are 2 major causes of failure in the characterization of DNA recovered from semen Offprint requests to: G. Pappalardo samples [1]. Low yields and partial restriction are 2 major causes of failure in the characterization of DNA recovered from semen Offprint requests to: G. Pappalardo samples [1].…”

Section: Introductionmentioning

confidence: 99%

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A common disinfectant used in condom processing inhibits endonuclease digestion of sperm DNA

1991

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DNA recovered from a condom found at a crime scene appeared undigestible with restriction enzymes, preventing characterization by Southern blot and polymorphic probe hybridization. Several chemical substances used in the processing and treatment of condoms were tested for inhibitory action of restriction enzymes. In particular dibenzalkonium chloride appeared to promote enzyme inhibition at very low concentrations. The effectiveness of treatments to restore cleavage of sample DNA in the presence of such contaminants is discussed.

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Section: Methodsmentioning

confidence: 99%

Section: Dna Restriction Electrophoresis Blotting and Characterizatmentioning

confidence: 99%