DNA Polymorphisms of the Lipoprotein Lipase Gene and Their Association with Coronary Artery Disease in the Saudi Population

Abstract

Coronary heart disease (CHD) is a major health problem and a major cause of death in most countries. Evidence has been presented that gene polymorphisms (HindIII, PvuII and Ser447Ter) of lipoprotein lipase (LPL) are risk factors of coronary artery disease (CAD).AimOur objective of the present investigation was to determine whether 3 LPL polymorphisms (LPL-HindIII, LPL-PvuII and LPL-Ser447Ter) can be considered as independent risk factors for CAD in the Saudi population.MethodsWe recruited 120 CAD subjects, confirmed angiographically with identical ethnic backgrounds and 65 control subjects. Polymerase chain reaction-restriction fragment length polymorphisms (RFLP) technique was used to detect the polymorphisms of the LPL gene.Results and conclusionFor the HindIII genotype, within the CAD group, the frequencies of the H+H+ were found in 50.8%, whereas 44.2% carried the H−H+ genotype, and 5% carried the H−H− genotype. Within the control group, the H+H+ genotype was found in 44.6%, whereas 35.4% carried the H−H+ genotype, 20% carried the H−H− genotype. The odds ratio (OR) of HindIII genotype H+H+ vs. H−H− genotype at 95% Confidence Interval (CI) were 4.6 (1.57–13.2) and p < 0.005, hence showing no significant association with CAD. For the PvuII genotype, within the CAD group the frequencies of the P+P+ found in 41.7% whereas 43.3.2% carried the P−P+ genotype, and 15% carried the P−P− genotype. Within the control group the P+P+ was found in 38.5%, 43.0% carried the P−P+ genotype, and 18.5% carried the P−P− genotype. The OR of PvuII genotype P+P+ vs. P−P− genotypes (95% CI) is 1.33 and p = 0.52; hence, it was also insignificant to show association with the disease. For the Ser447Ter genotype, within the CAD group, the frequencies of the C/C found in 83.3%, whereas 16.7% carried the C/G genotype. Within the control group, the C/C was found in 87.7% and 12.3% carried the C/G genotype. We did not get any GG genotypes in control as well as patients for this gene. It can be concluded that C allele of gene masks the presence of G allele in the Saudi population. The OR of CG + GG vs. CC (95% CI) is 1.43 from 0.59 to 3.44 which is insignificant. Hence this gene also has no significant association with CAD in the Saudi population.

Effect of Polymorphism Ser447stop Lipoprotein Lipase Gene on the Concentration of Testosterone in Male is Infertile in the City of Samarra

“…This result were in agreement with observation of [33] which found that the frequency of the S447 was significantly higher in healthy subjects than in coronary artery disease, while the frequency of the X447allele was significantly higher in coronary artery disease as it amounted to rate of 83% compared to healthy group as amounted to rate of 61%. It also found [34] that the patient's with cardiovascular the Turkish they had a high in the frequency of the X447 allele as amounted to rate of 29.2% compared to healthy group as amounted to rate of allele frequency X447 of 17.39% .…”

Lipoprotein Lipase (LPL) Polymorphism and the Risk of Coronary Artery Disease: A Meta-Analysis

“…A few studies have reported that the polymorphisms of HindIII, Ser447X and PvuII were associated with the risk of CAD [ 10 , 12 , 13 , 14 ]. However, these results were also controversial [ 14 , 15 , 16 , 17 ]. Some studies found no association between these gene variants of LPL and the risk of CAD [ 14 , 15 ].…”

“…However, these results were also controversial [ 14 , 15 , 16 , 17 ]. Some studies found no association between these gene variants of LPL and the risk of CAD [ 14 , 15 ].…”

Hindiii and S447x Polymorphisms of Lipoprotein Lipase Gene and Their Relationship to Coronary Artery Disease

“…Coronary artery disease (CAD) is one of the greatest causes of morbidity and mortality worldwide being responsible for a high percentage of death annually in different countries (Al-Jafari et al, 2012). CAD is a multifactorial disorder believed to result from an interaction between the genetic background and environmental factors such as diet, smoking and physical activity (Daoud et al, 2013).…”

“…The disease is usually associated with conventional risk factors including hypertension, diabetes mellitus, dyslipidemia and smoking (Rustempasic et al, 2014). In recent years, many studies started to explore the relationship between CAD and the common variants of genes central to lipid metabolism (Al-Jafari et al, 2012andTurlo et al, 2014. The lipoprotein lipase (LPL) gene, which spans; 35 kilobases on chromosome 8p22 is composed of 10 exons separated by 9 introns and encodes the mature form of LPL (Tanguturi et al, 2013).…”