DNA polymorphisms in new isolates of 'Deinococcus radiopugnans'

Masters, C. I.; Murray, R. G. E.; Moseley, Bevan; Minton, Kenneth W.

doi:10.1099/00221287-137-7-1459

Cited by 21 publications

(11 citation statements)

References 23 publications

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“…The radiation resistance values obtained in this study for Deinococcus radiodurans were comparable to those obtained previously for this organism (32,33). The different radiation resistance of strains AG3aT and ALT-lbT may not reflect a taxonomic distinction between the two species, but may be due to differences among D. radioduransl strains, as was shown for several strains assigned to Deinococcus radiopugnans (28).…”

Section: Discussionmentioning

confidence: 98%

“…The most striking characteristic of the species of this genus is their extreme resistance to UV and gamma radiation. For this reason UV or gamma radiation has been used to isolate most of the organisms of the genus Deinococcus from foods, feces, soils, and dust (1,8,19,22,24,28,36). The small number of strains isolated without irradiation of samples demonstrates that the extreme radiation resistance of the organisms is not a result of selection of resistant strains by irradiation, but is a normal characteristic, which is believed to be related to the desiccation resistance of these organisms (29).…”

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confidence: 99%

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Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., Two Extremely Radiation-Resistant and Slightly Thermophilic Species from Hot Springs

Ferreira

Nobre

Rainey

et al. 1997

International Journal of Systematic Bacteriology

213

206

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Strains ofThe genus Deinococcus includes the species Deinococcus radiodurans, Deinococcus proteolyticus, Deinococcus radiophilus, and Deinococcus radiopugnans. The species Deinococcus erythromyxa was also included in this genus as a species incertae sedis by Brooks and Murray (3). The rod-shaped organisms of the species Deinococcus grandis are very closely related to the species of the genus Deinococcus, but were classified in the genus Deinococcus primarily on the basis of morphology (36). Recently, the type strains of these species were subjected to a complete 16s ribosomal DNA (rDNA) sequence analysis, which resulted in the reclassification of Deinococcus grandis and Deinococcus erythromyxa (41). The species Deinococcus grandis, despite its rod-shaped morphology, falls within the radiation of the genus Deinococcus and for this reason was classified as Deinococcus grandis. The species Deinococcus erythromyxa was known by Brooks and Murray (3) to have characteristics that were different from those of the other species of Deinococcus, and subsequent studies confirmed its chemotaxonomic distinctiveness, which reinforced the need for reclassification of this species (12). The species Deinococcus erythromyxa has been found to be phylogenetically very closely related to the gram-positive species Kocuria rosea, but due to the low DNA-DNA hybridization values between the two species, Kocuria erythromyxa was maintained as a separate speciesThe species of the genus Deinococcus are strictly aerobic, have optimum growth temperatures in the range from 25 to 35"C, produce reddish colonies, generally stain gram positive, have ornithine in the peptidoglycan, lack teichoic acids, possess menaquinone 8 as the major respiratory quinone, and are (41).

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Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., Two Extremely Radiation-Resistant and Slightly Thermophilic Species from Hot Springs

Ferreira

Nobre

Rainey

et al. 1997

International Journal of Systematic Bacteriology

213

206

View full text Add to dashboard Cite

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“…The genus Deinococcus, which represents a deep-branching lineage within the Bacteria, comprises 26 species with validly published names at the time of writing, isolated from various environments such as desert soils, foods, faeces, dust, hot springs, Antarctic environments and the plant rhizosphere (Rainey et al, 2005;Ferreira et al, 1997;Lai et al, 2006;Weon et al, 2007;Anderson et al, 1956;Davis et al, 1963;Lewis, 1973;Masters et al, 1991;Oyaizu et al, 1987;Hirsch et al, 2004). The most striking characteristic of species of the genus is their extreme resistance to UV light, gamma radiation and desiccation.…”

mentioning

confidence: 99%

Deinococcus xinjiangensis sp. nov., isolated from desert soil

Peng¹,

Zhang²,

Luo³

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A Gram-positive-staining, spherical-shaped and faintly pink-pigmented bacterial strain, X-82 T , was isolated from soil samples collected from a desert in Xinjiang, China. The organism was found to be resistant to UV radiation but sensitive to gamma radiation and desiccation. The optimum growth pH, NaCl concentration and temperature were pH 7.0, 0-1 % NaCl and 30 6C. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain X-82 T is member of a novel species belonging to the genus Deinococcus, with Deinococcus hopiensis KR-140 T as its closest relative (93.5 % similarity). The DNA G+C content (60 mol%), quinone type (MK-8), major cellular fatty acids (16 : 1v7c, 16 : 0 and 17 : 1 iso v9c), the presence of peptidoglycan with L-ornithine and the dominant polar lipid (phosphoglycolipids and glycolipids) support the affiliation of strain X-82 T with the genus Deinococcus. A novel species is proposed, for which the name Deinococcus xinjiangensis sp. nov. is proposed, with the type strain X-82 T

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“…This can reflect differences between isolates and is not a distinctive taxonomic feature. Resistance to radiation can be different in each strain of Deinococcus (Masters et al 1991).…”

Section: Radiation Resistancementioning

confidence: 99%

Radiation resistant of native Deinococcus spp. isolated from the Lout desert of Iran “the hottest place on Earth”

Mohseni

Abbaszadeh

Omran

2014

Int. J. Environ. Sci. Technol.

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Two native ionizing radiation-resistant bacteria were isolated and identified from a soil sample collected from extreme conditions of the Lout desert in Iran. The hottest land surface temperature has been recorded in the Lout desert from 2004 to 2009. Also, it is categorized as a hyper arid place. Both ionizing radiation and desiccation may cause damage on genome. Soil sample was irradiated in order to eliminate sensitive bacteria then cultured in onetenth-strength tryptic soy broth medium. Bacterial suspension used for radiation treatment. Morphological and physiological characterization and phylogenetic studies based on 16S rRNA gene sequence were used for identification. The cells were rod shape, non-motile, non-spore forming and gram positive. The 16S rRNA gene sequence showed 99.5 % of similarity to Deinococcus ficus. Phylogenetic dendrogram demonstrated that the isolates branched with D. xibeiensis, D. ficus and D. mumbaiensis. Both isolates were resistant to [15 kGy of gamma radiation and [600 J m 2 of UV radiation. This is the first report on radiation resistant bacteria belonging to genus Deinococcus isolated from the Lout desert of Iran.

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DNA polymorphisms in new isolates of 'Deinococcus radiopugnans'

Cited by 21 publications

References 23 publications

Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., Two Extremely Radiation-Resistant and Slightly Thermophilic Species from Hot Springs

Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., Two Extremely Radiation-Resistant and Slightly Thermophilic Species from Hot Springs

Deinococcus xinjiangensis sp. nov., isolated from desert soil

Radiation resistant of native Deinococcus spp. isolated from the Lout desert of Iran “the hottest place on Earth”

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