DNA Polymorphism of Chloroplast SSR Regions in Various Vegetables and its Application to Analyses of Interspecific Polymorphisms in Allium species

Abstract

Polymorphic analyses of simple sequence repeat regions on chloroplast genomes in 34 vegetables were conducted using a set of consensus SSR primer pairs. A sufficient number of DNA markers were obtained in this study and could be available for polymorphic analyses at the species level. The most successful result was obtained from the polymorphic analyses of cultivated and wild species in Allium. The DNA fingerprints of Allium facilitated the identification of each species except in one difficult case. The fingerprint of a natural hybrid, A. × wakegi, could not be distinguished from that of its estimated seed parent, A. fistulosum. The DNA markers obtained from this study seem to have potential not only for breeding purposes but also for cultivar identification in various species of vegetables.

Production of novel alloplasmic male sterile lines in Allium cepa harbouring the cytoplasm from Allium roylei

“…Total genomic DNA was isolated from young fresh leaf tissue using a mini-prep DNA isolation method (Van Heusden et al 2000a). In the study reported by Araki et al (2010a), the DNA polymorphism in various Allium species was detected by using a set of consensus SSR primer pairs developed by Chung and Staub (2003). The analyses based on the two primer sets ccSSR11 and ccSSR17 were used to distinguish chloroplast genomes in A. roylei from those in shallot ÔChiang MaiÕ and bulb onion ÔKitami Kohai 39 goÕ.…”

“…2000a). In the study reported by Araki et al. (2010a), the DNA polymorphism in various Allium species was detected by using a set of consensus SSR primer pairs developed by Chung and Staub (2003).…”