DNA-Polyamine Interactions: Insight from Molecular Dynamics Simulations on the Sequence-Specific Binding of Spermidine3+

Mocci, Francesca; Laaksonen, Aatto; Engelbrecht, Leon; Vasiliu, Tudor; Perepelytsya, S. M.

doi:10.1007/978-3-030-80924-9_6

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2023

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“…Still a more intimate contact between quarternized nitrogen atoms in PA and phosphate groups in DNA is possible (Cherng, 2009). When N/P ratio exceeds 10, the positively charged polyplexes are formed and the excess PA causes DNA condensation (Jorge, Dias, Pereira, & Pais, 2010;Mocci, Laaksonen, Engelbrecht, Vasiliu, & Perepelytsya, 2022). The Δf and ∆D curves soon level-off because strongly protonated state need shorter time for complex formation (see segments DE and de in Figure 1).…”

Section: Resultsmentioning

confidence: 99%

DNA Release from Polyaziridine Polyplexes Aided by Biomacromolecules: Effect of pH

Bibi,

Shah

2023

Acta Sci Technol

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The pH dependence of heparin and dextran sulfate aided release of DNA from its polyplexes with polyaziridine (PA) has been investigated using Quartz crystal microbalance with dissipation monitoring (QCM-D). The acoustic behavior of ternary systems indicated that the DNA decondensation was favored at physiological pH. When pH < 7.4, PA was significantly positively charged and ternary complexation occurred. When pH exceeded 7.4, very small proportion of DNA was released upon heparin addition due to marked reduction in cationic nature of PA. An increase in thickness at the interface due to adsorbed material at pH 9.0 showed the retention of heparin on the surface despite of negligible release of DNA. In terms of PA binding, heparin proved to be a better competitor than dextran sulfate.

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Section: Resultsmentioning

confidence: 99%