DNA ploidy status in 84 ocular melanomas: A study of dna quantitation in ocular melanomas by flow cytometry and automatic and interactive static image analysis

Coleman, Kate; Baak, J. P. A.; Diest, Paul J. van; Curran, B.; Mullaney, Joan; Fenton, M.; Leader, M.

doi:10.1016/0046-8177(95)90121-3

Cited by 21 publications

(11 citation statements)

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“…al., 1995). Interestingly, the spindle A cell type has been characterized as strictly diploid, whereas the spindle B cell type has been characterized as having increased aneuploidy (Coleman et al, 1995). Aneuploidy of UM is a risk factor for worse prognosis (Karlsson, et al, 1996), and increased mortality (Karlsson, Boeryd, Carstensen, Kagedal, & Wingren, 1995).…”

Section: Cytogenetic Evaluation Of Uveal Melanoma Aneuploidymentioning

confidence: 99%

Genomic Investigations of Posterior Uveal Melanoma

Hovland

Trempe²

2005

Seminars in Ophthalmology

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The specific genetic mechanisms responsible for the malignant behavior of uveal melanoma are not known. Unlike cutaneous melanoma, epidemiologic studies have not demonstrated a definitive germline form of uveal melanoma, though familial melanoma and racial predilections occur. Molecular cytogenetic characterization of uveal melanoma suggests that somatic deletions of chromosome 3 are associated with a worse prognosis. Microarray technology has been used to characterize uveal melanoma gene expression and may provide tests useful for determining prognosis. As an improved understanding of the cellular mechanisms used by uveal melanoma is gained, new opportunities to adapt or design therapeutic approaches may emerge.

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Section: Cytogenetic Evaluation Of Uveal Melanoma Aneuploidymentioning

confidence: 99%

Genomic Investigations of Posterior Uveal Melanoma

Hovland

Trempe²

2005

Seminars in Ophthalmology

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“…Unfortunately, it lacks reproducibility, primarily because there is no objective cut off point that could be used to categorise tumours within the mixed cell class 56 Moreover, it does not differentiate between the various tumours within the different classes 5…”

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confidence: 99%

DNA ploidy pattern in choroidal melanoma: correlation with survival. A flow cytometry study on archival material

Toti

Greco

Mangiavacchi

et al. 1998

British Journal of Ophthalmology

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Background/aims-ParaYn embedded samples have provided an important source of material for retrospective cytofluorimetric studies, useful in establishing the predictive value of DNA content measurements. The aim of this study was to investigate the incidence and type of aneuploidy in choroidal malignant melanomas (CMM) and the significance in the clinical outcome (median follow up 55 months). Methods-DNA content was quantified by flow cytometry in 61 CMM from archival material. Non-tumour ocular tissue was used as the reference diploid standard. Cases in which the coeYcient of variation (CV) of the diploid peak was >8% were excluded. The CMM were classified as spindle A, spindle B, mixed spindle and epithelioid, epithelioid, and necrotic. Results-The frequency of the aneuploid DNA pattern was 38%. Necrotic tumours showed a worse clinical outcome independent of the ploidy pattern. Spindle A tumours were found to be diploid. Spindle B and mixed tumours showed a prevalent diploid and near diploid aneuploid pattern (DI <1.3), yet aneuploidy was not correlated with a worse prognosis. The epithelioid tumours were prevalently diploid. However, 83% of the aneuploid tumours were hypodiploid (DI <0.95), and showed the worst prognosis. Conclusion-These results indicate that increasing DNA abnormalities in CMM, especially in the epithelioid histotype, were associated with an increasing mortality. (Br J Ophthalmol 1998;82:1433-1437 Choroidal malignant melanoma (CMM) is the most common primary intraocular malignancy in adults. The estimated 15 year survival rate after detection of the tumour is 53%.

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“…For uveal melanoma it has been shown that nuclear DNA content (ploidy) abnormalities correlate closely with survival,1-4 although conflicting results have been reported for older archival material,5 fresh tissue,6 and differences in DNA quantification techniques 7. A significant correlation between aneuploidy and epithelioid cell type has been reported2 3 8; other studies indicate similar findings,9 or contradict these findings 4.…”

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confidence: 99%

“…Comparing IA and FCM on uveal melanomas, tetraploidy was detected in tumours that were diploid by FCM 12. By IA it was found that all spindle A cells were diploid,7 12 and most tetraploid peaks were formed by epithelioid cells 12. The lack of association of aneuploidy with epithelioid cell type may partly be explained by the application of a modified (five categories) classification (see Toti et al ).…”

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confidence: 99%

“…Because of the above named restrictions, the recommended FCM ploidy classification on paraffin embedded tissue from solid tumours is diploid versus non-diploid (aneuploid) 11. Conflicting results on the prognostic value of DNA aneuploidy can be attributed to differences in methods6 7 and definition of aneuploidy (Toti et al ). Difficult areas in flow histograms, specifically high CV, high G2M phase, as well as near diploid aneuploidy and hypodiploidy can be clarified by IA,16 or in frozen tissue by FCM 11…”

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confidence: 99%

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