DNA packaging initiation of Salmonella bacteriophage P22: determination of cut sites within the DNA sequence coding for gene 3

Backhaus, Horst

doi:10.1128/jvi.55.2.458-465.1985

Cited by 44 publications

(5 citation statements)

References 52 publications

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“…Bacteriophage P22 DNA packaging series are known to initiate at DNA ends generated within gene 3 (Casjens and Huang, 1982; Backhaus, 1985; Casjens et al ., 1992b), and we previously noted that a particular sequence in gene 3 could be the pac site, as it is present near the packaging series initiation point in P22 wild type and near the two different packaging series initiation points programmed by the mutant gene 3 protein of P22 3 – HT12/4a (Casjens et al ., 1987). The work of Weaver and Levine (1978) and of Schmieger and co‐workers (Kufer et al ., 1982; Backhaus, 1985; Petri and Schmieger, 1990) on the packaging of excision defective prophages and plasmid DNAs, respectively, supports the notion that the recognition site is near gene 3 . We tested pac site identification system one (described above; Fig.…”

Section: Resultsmentioning

confidence: 99%

The DNA site utilized by bacteriophage P22 for initiation of DNA packaging

Sampson

Parr

et al. 2002

Molecular Microbiology

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SummaryVirion proteins recognize their cognate nucleic acid for encapsidation into virions through recognition of a specific nucleotide sequence contained within that nucleic acid. Viruses like bacteriophage P22, which have partially circularly permuted, double-stranded virion DNAs, encapsidate DNA through processive series of packaging events in which DNA is recognized for packaging only once at the beginning of the series. Thus a single DNA recognition event programmes the encapsidation of multiple virion chromosomes. The protein product of P22 gene 3 , a terminase component, is thought to be responsible for this recognition. The site on the P22 genome that is recognized by the gene 3 protein to initiate packaging series is called the pac site. We report here a strategy for assaying pac site activity in vivo , and the utilization of this system to identify and characterize the site genetically. It is an asymmetric site that spans 22 basepairs and is located near the centre of P22 gene 3 .

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Section: Resultsmentioning

confidence: 99%

The DNA site utilized by bacteriophage P22 for initiation of DNA packaging

Sampson

Parr

et al. 2002

Molecular Microbiology

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“…UL15 is highly conserved among the herpesvirus family, and sequence analysis indicates a possible homology between the UL15 protein of HSV-1 and the large subunit (gp17) of the terminase complex of bacteriophage T4 (17). The most significant aspect of this homology is the presence of a putative nucleotide binding fold (Walker boxes A and B) in both proteins (19,57), suggesting that UL15 may be able to bind and hydrolyze ATP as has been demonstrated for gp17 and other phage terminases (5,10,12).…”

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confidence: 89%

Characterization of ICP6::lacZ insertion mutants of the UL15 gene of herpes simplex virus type 1 reveals the translation of two proteins

Yü

Sheaffer

Tenney

et al. 1997

J Virol

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The herpes simplex virus type 1 (HSV-1) UL15 gene is a spliced gene composed of two exons and is predicted to encode an 81-kDa protein of 735 amino acids (aa). Two UL15 gene products with molecular masses of 75 and 35 kDa have been observed (J. Baines, A. Poon, J. Rovnak, and B. Roizman, J. Virol. 68:8118-8124, 1994); however, it is not clear whether the smaller form represents a proteolytic cleavage product of the larger form or whether it is separately translated. In addition, an HSV-1 temperature-sensitive mutant in the UL15 gene (ts66.4) is defective in both cleavage of viral DNA concatemers into unit-length monomers and packaging of viral DNA into capsids (A.

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“…25 A terminase complex consisting of gene products gp2 and gp3 recognize a site within gene 3 (termed pac) and cleaves the DNA to allow packaging initiation. 57,72,73 Packaging then proceeds``rightward'' toward the head genes. 57,74,75 After the phage head has been ®lled with DNA, the concatemer is imprecisely cleaved in a region past the next pac site the P22 Portal Vertex Formation in Vivo free DNA end is then used to initiate packaging into another procapsid.…”

Section: Excess Portals Block Dna Packagingmentioning

confidence: 99%