DNA Origami Nanoarrays for Multivalent Investigations of Cancer Cell Spreading with Nanoscale Spatial Resolution and Single-Molecule Control

Huang, Dan; Patel, Ketan; Perez‐Garrido, Sandra; Marshall, John F.; Palma, Matteo

doi:10.1021/acsnano.8b08010

Cited by 67 publications

(62 citation statements)

References 53 publications

(91 reference statements)

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“…ChemBioChem 2020, 21,[33][34][35][36][37][38][39][40][41][42][43][44] www.chembiochem.org increased by around 15 %( from 68.3, 71.8 and 70.5 %t o8 5.8, 86.2 and 86.3 %f or cross, rectangle and triangle shapes,r espectively) if the reactionw as performed at an elevated temperatureo f3 78C, relative to the results obtained at commonly used room temperature.…”

Section: Introductionmentioning

confidence: 87%

“…ChemBioChem 2020, 21,[33][34][35][36][37][38][39][40][41][42][43][44] www.chembiochem.org toplasm roughly in the same order of magnitude as lipofectamine-based transfection". Althought hese results are indeed significant,i ti sl ikely that that the nanostructures, once internalised, presumably endu pi ne ndosomes, where they cannot perform their intended function, in most cases.I tt herefore remains to be seen how effectively this strategy can ultimately offer improvementi nt he activity of the nanodevice, despite reports of significant advancementinu ptake efficiency.…”

Section: Strategies To Enhance Stability and Uptakementioning

confidence: 99%

“…Another study that takes this approach of spatial control of ligand presentation on the cell membrane is a very recent study in which Huang and co‐workers used lithographic techniques to construct nanoarrays, in which they were able to position triangular DNA origami structures that exhibited cell‐adhesion ligands spaced 60 nm apart, in this case for integrin and epidermal growth factor (EGF), in different ways, which then modulated epidermal cancer cell behaviour . The authors found that A20FMDV2, which was an integrin‐binding ligand, and EGF showed positive cooperation in promoting the spread of cancer cells that overexpressed integrin.…”

Section: Special Applications In Interfacing With the Cellmentioning

confidence: 99%

“…Therefore, ligand positioning is an important parameter that determines how these pathways are activated. Indeed, the authors found that the ChemBioChem 2020, 21,[33][34][35][36][37][38][39][40][41][42][43][44] www.chembiochem.org nanoscale spacingo fe phrin-A5 directed the levels of EphA2 receptor activation in human breast cancer cells. They have also shown that the distribution of ephrin-A5r egulates the invasive properties of breast cancerc ells.…”

Section: Cell-nanostructureinteractionsmentioning

confidence: 99%

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Advances in DNA Origami–Cell Interfaces

et al. 2019

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The nascent field of DNA nanotechnology has undergone rapid growth since its inception. By using DNA as a biologically “safe” material, DNA nanotechnology shows great promise in applications such as drug‐delivery systems. Further progress, however, relies on understanding the different ways in which DNA nanostructures behave in and interact with cells, tissues and even whole organisms. Moreover, this knowledge must then be harnessed in innovative ways to improve existing DNA nanostructures and design new ones, so that they can perform more diverse functions more effectively. There have been many developments in this regard in the past few years, and herein some of these are highlighted, with respect to both works that improve our understanding of what happens to DNA nanostructures once they are at their target site, and those that utilise clever design to accomplish desired functions.

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Section: Introductionmentioning

confidence: 87%

Section: Strategies To Enhance Stability and Uptakementioning

confidence: 99%

Section: Special Applications In Interfacing With the Cellmentioning

confidence: 99%

Section: Cell-nanostructureinteractionsmentioning

confidence: 99%

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Advances in DNA Origami–Cell Interfaces

et al. 2019

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“…In addition, DNA origami-based multivalent nanomodels unraveled cooperative functions of EGF and integrin ligands immobilized at distances of 60 nm. These insights highlight the importance of cooperative ligand functions for central tumor cell functions such as proliferation and spreading [73].…”

Section: Modulation Of Tumor Cell Functions By Nanostructured Ligandsmentioning

confidence: 94%

Modulating Tumor Cell Functions by Tunable Nanopatterned Ligand Presentation

Amschler

Schön

2020

Nanomaterials

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Cancer comprises a large group of complex diseases which arise from the misrouted interplay of mutated cells with other cells and the extracellular matrix. The extracellular matrix is a highly dynamic structure providing biochemical and biophysical cues that regulate tumor cell behavior. While the relevance of biochemical signals has been appreciated, the complex input of biophysical properties like the variation of ligand density and distribution is a relatively new field in cancer research. Nanotechnology has become a very promising tool to mimic the physiological dimension of biophysical signals and their positive (i.e., growth-promoting) and negative (i.e., anti-tumoral or cytotoxic) effects on cellular functions. Here, we review tumor-associated cellular functions such as proliferation, epithelial-mesenchymal transition (EMT), invasion, and phenotype switch that are regulated by biophysical parameters such as ligand density or substrate elasticity. We also address the question of how such factors exert inhibitory or even toxic effects upon tumor cells. We describe three principles of nanostructured model systems based on block copolymer nanolithography, electron beam lithography, and DNA origami that have contributed to our understanding of how biophysical signals direct cancer cell fate.

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Biointerface Engineering with Nucleic Acid Materials for Biosensing Applications

Shi

Chen

Wang

et al. 2022

Adv Funct Materials

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Molecular recognition at the biointerface plays a critical role in sensing molecular interactions (e.g., DNA hybridization) and extracellular changes, which can directly affect the detection performance of biosensors (e.g., sensitivity, specificity, and response dynamics). However, conventional sensing biointerfaces show low molecular recognition efficiency due to limited target accessibility. Engineering sensing biointerfaces to regulate the orientation, spacing, and density of surface‐confined molecular probes offer an effective approach to improve molecular recognition at interfaces. Over the last decades, biointerface engineering with nucleic acid materials has advanced the fundamental understanding of DNA hybridization kinetics and facilitated the design of improved biosensing platforms for monitoring cellular activities and diagnosing relevant diseases. This review summarizes the recent progress in nucleic acid‐based biointerface engineering. The development of nucleic acid materials that can be applied to specific diagnostic applications is briefly introduced. Then the roles of nucleic acids in tailoring the properties of nanosurfaces, cell surfaces, and macroscopic surfaces are discussed and their biosensing applications are comprehensively highlighted. Finally, future challenges and perspectives of emerging technologies and applications in the field are presented.

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DNA Origami Nanoarrays for Multivalent Investigations of Cancer Cell Spreading with Nanoscale Spatial Resolution and Single-Molecule Control

Cited by 67 publications

References 53 publications

Advances in DNA Origami–Cell Interfaces

Advances in DNA Origami–Cell Interfaces

Modulating Tumor Cell Functions by Tunable Nanopatterned Ligand Presentation

Biointerface Engineering with Nucleic Acid Materials for Biosensing Applications

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