DNA nanotechnology: a future perspective

Zahid, Maria; Kim, Byeong‐Hoon; Hussain, Rafaqat; Amin, Rashid; Park, Sungha

doi:10.1186/1556-276x-8-119

Cited by 56 publications

(34 citation statements)

References 93 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Deoxyribonucleic acids (DNAs) are one of the major building blocks of biological systems. Recently, researchers have been exploiting DNA self-assembly for constructing nanostructures as well as programming molecular devices [1][2][3][4][5][6][7][8][9][10][11][12][13]. Yurke et al first observed the phenomenon of DNA strand displacement in their nanomachine [14].…”

Section: Introductionmentioning

confidence: 99%

“…Lanes 2, 3, and 4 contain a mixture of two hairpins (H 6-7 ), three hairpins (H 6-8 ), and four hairpins (H 6-9 ), respectively without the initiator: products of high molecular weight are slightly visible, indicating evidence of some leak. Lanes 5, 6, 7 contain a mixture of H 6-7 , H[6][7][8] , and H 6-9 , respectively with the initiator: products of high molecular weight are visible, indicating strong evidence of linear cascade reactions. A mismatch of hairpin concentrations is also visible in lines 7 and 8.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Design and analysis of linear cascade DNA hybridization chain reactions using DNA hairpins

et al. 2017

View full text Add to dashboard Cite

DNA self-assembly has been employed non-conventionally to construct nanoscale structures and dynamic nanoscale machines. The technique of hybridization chain reactions by triggered selfassembly has been shown to form various interesting nanoscale structures ranging from simple linear DNA oligomers to dendritic DNA structures. Inspired by earlier triggered self-assembly works, we present a system for controlled self-assembly of linear cascade DNA hybridization chain reactions using nine distinct DNA hairpins. NUPACK is employed to assist in designing DNA sequences and Matlab has been used to simulate DNA hairpin interactions. Gel electrophoresis and ensemble fluorescence reaction kinetics data indicate strong evidence of linear cascade DNA hybridization chain reactions. The half-time completion of the proposed linear cascade reactions indicates a linear dependency on the number of hairpins.

show abstract

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Design and analysis of linear cascade DNA hybridization chain reactions using DNA hairpins

et al. 2017

View full text Add to dashboard Cite

show abstract

“…To achieve these detection methods, plant pathologists are working with nanotechnologists to develop quick and better detection systems (Sharon et al, 2010). DNA has also become a preferred material for nanotechnologists because of its unique properties of structural stability, programmability of sequences, and predictable selfassembly (Zahid et al, 2013). Magnetic nanoparticles (MNPs) consisting of magnetite (Fe3O4) are promising as nanodiagnostic tools in plant pathology (Alghuthaymi et al, 2016;Khiyami et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Nanotools for molecular identification two novels Cladosporium cladosporioides species (Cladosporiaceae) collected from tomato phyloplane

Alghuthaymi¹

2017

J. Yeast Fungal Res.

View full text Add to dashboard Cite

Magnetic nanoparticles (MNPs) consisting of magnetite (Fe 3 O 4 ) are promising as nanodiagnostic tools in plant pathology. Magnetic nanoparticles (MNPs) were produced using hydrothermal protocols. MNPs size diameter and size distribution were characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). Fe 3 O 4 nanoparticles are monodispersible and spherical with an average diameter of 82 nm. Dynamic light scattering analysis of the same samples revealed that the synthesized MNPs were highly monodispersed and had a hydrodynamic diameter ranging from 100 to 201. Fungal DNA was extracted using MNPs in comparison with the conventional sodium dodecyl sulfate (SDS) method in the context of quality, quantity and timing process. The quality and yields of the isolated DNA from all Cladosporium strains using magnetic nanoparticles were higher compared to the DNA isolation method via sodium dodecyl sulfate (SDS). PCR using specific primers targeting ITS and Actin genes were amplified 100% of varying sized gene fragments, verifying the high quality of the isolated DNA. Forty seven fungal isolates belonging to the Cladosporium cladosporioides complex were observed and phylogenetically evaluated on the basis of DNA sequences of the internal transcribed spacer regions ITS1 and ITS2, in addition to partial actin and translation elongation factor 1-α gene sequences. Maximum likelihood phylogenetic analyses were performed for the combined data set (ITS + ACT + TEF) using RAxML. The three Cladosporium strains were isolated from tomato in Saudi and Egypt was identified very similar to Cladosporium asperulatum and Cladosporium myrtacearum based on their molecular phylogenetic characteristics. DNA isolation using magnetic nanoparticles will expectedly be used commonly both in plant pathology laboratories and in the nanobiotechnology industry.

show abstract

“…This paper seeks to identify and address the many experimental problems to which the DNA double helix model seems to offer no solution such as the inevitable topological complexity of the DNA octahedron (Zahid et al, 2013). Thus the body of DNA studies in nanotechnology can be strengthened and fresh approaches to potential developments in DNA nanotechnology can be identified.…”

Section: Introductionmentioning

confidence: 99%

“…Though the DNA cube, and DNA octahedron (Zahid et al, 2013) for example, are well known and have been drawn in detail, the evident complexity of such molecules, and other, comparable constructs using the double helix, seems to constitute a formidable obstacle to attempts to further develop such concepts and to widen the use of such DNA constructs in medicine, computing and in nanorobotics.…”

Section: Introductionmentioning

confidence: 99%

Extending the Repertoire of Structures in DNA Nanotechnology

Delmonte¹

2015

American Journal of Nanotechnology

View full text Add to dashboard Cite

DNA nanotechnology remains an active area of research and advances have been reviewed recently. DNA nanotechnology seeks to deploy molecules at an atomic level and on a small molecule scale. Other techniques in biophysics and biochemistry do not need to address the issue of the true structure of the nucleic acids at an atomic level but, rather, at a macro-atomic level such as in genetics and in immunology, for example. Accordingly, DNA nanotechnology is perhaps uniquely dependent upon exact clarity in the secondary and tertiary structures of the nucleic acids, as well as that can ever be achieved. Challenges include expanding the use of DNA in medicine, and the construction of detectors with higher sensitivity for biological and chemical settings. Though increasingly complex architectures have been constructed, novel approaches to a greater rôle in biological computation and data storage remain important goals. Here a repertoire of structures for DNA at an atomic level is described which offers a new conjecture with which to move forward. The DNA double helix model faces many problems which have become apparent in the 62 years of research in molecular biology that have elapsed since it was formulated by Watson and Crick in 1953. Experimental evidence is set out seeking to show that the only truly side-by-side alternative, the paranemic model, accounts better for the wide range of phenomena otherwise inexplicable with the double helix model. This paranemic model can engage in a repertoire of structural options denied to the DNA double helix model. Without the requirement to postulate unwinding of the DNA strands, the nucleotide base sequence is immediately accessible to complementary DNA sequences to promote rapid detection of specific molecules in biological and medical settings. Rapid switching between Watson-Crick and Hoogsteen base pairing and four-stranded structures can allow greater complexity in the construction of molecular switches and digital programming.

show abstract

DNA nanotechnology: a future perspective

Cited by 56 publications

References 93 publications

Design and analysis of linear cascade DNA hybridization chain reactions using DNA hairpins

Design and analysis of linear cascade DNA hybridization chain reactions using DNA hairpins

Nanotools for molecular identification two novels Cladosporium cladosporioides species (Cladosporiaceae) collected from tomato phyloplane

Extending the Repertoire of Structures in DNA Nanotechnology

Contact Info

Product

Resources

About