DNA Nanobiosensors: An Outlook on Signal Readout Strategies

Chandrasekaran, Arun Richard

doi:10.1155/2017/2820619

Cited by 23 publications

(13 citation statements)

References 92 publications

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“…These signal readers are integrated electronically with smart gadgets or plate readers to measure fluorescence. The improvement in signal detection methods improves the sensitivity of the procedure [ 110 ]. The conventional signal readout method is highly delicate to interpret the final results.…”

Section: Detection Techniques Of Sars-cov-2mentioning

confidence: 99%

Advancements in Testing Strategies for COVID-19

et al. 2022

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The SARS-CoV-2 coronavirus, also known as the disease-causing agent for COVID-19, is a virulent pathogen that may infect people and certain animals. The global spread of COVID-19 and its emerging variation necessitates the development of rapid, reliable, simple, and low-cost diagnostic tools. Many methodologies and devices have been developed for the highly sensitive, selective, cost-effective, and rapid diagnosis of COVID-19. This review organizes the diagnosis platforms into four groups: imaging, molecular-based detection, serological testing, and biosensors. Each platform’s principle, advancement, utilization, and challenges for monitoring SARS-CoV-2 are discussed in detail. In addition, an overview of the impact of variants on detection, commercially available kits, and readout signal analysis has been presented. This review will expand our understanding of developing advanced diagnostic approaches to evolve into susceptible, precise, and reproducible technologies to combat any future outbreak.

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Section: Detection Techniques Of Sars-cov-2mentioning

confidence: 99%

Advancements in Testing Strategies for COVID-19

et al. 2022

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“…Recently, DNA nanostructures have garnered tremendous interest in biosensing due to their high surface-to-volume ratio, which provides greater space for responsive elements and therefore greater changes in signal generation. 251 This biosensing ability has been explored for the early detection of pathogens in human samples 262 with enhanced target specificity and avidity. 263 In this section, aptasensors utilizing a DNA nanostructure scaffold are discussed, categorizing the techniques based on the same output methods discussed previously.…”

Section: Role Of Dna Nanotechnology In Viral Diagnostics and Therapymentioning

confidence: 99%

Aptamers for Viral Detection and Inhibition

et al. 2022

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Recent times have experienced more than ever the impact of viral infections in humans. Viral infections are known to cause diseases not only in humans but also in plants and animals. Here, we have compiled the literature review of aptamers selected and used for detection and inhibition of viral infections in all three categories: humans, animals, and plants. This review gives an in-depth introduction to aptamers, different types of aptamer selection (SELEX) methodologies, the benefits of using aptamers over commonly used antibody-based strategies, and the structural and functional mechanism of aptasensors for viral detection and therapy. The review is organized based on the different characterization and read-out tools used to detect virus–aptasensor interactions with a detailed index of existing virus-targeting aptamers. Along with addressing recent developments, we also discuss a way forward with aptamers for DNA nanotechnology-based detection and treatment of viral diseases. Overall, this review will serve as a comprehensive resource for aptamer-based strategies in viral diagnostics and treatment.

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“…RNA/DNA nano-biosensors can measure the responses produced by aptamer hybridization or nucleic acid conversion processes as promising diagnostic tools [74]. As aptamers, RNA or DNA are single-stranded nucleic acid oligomers whose structure is extremely organized and complex, forming a strong connection with the target molecules (Figure 1) [75].…”

Section: Rna Nano-biosensorsmentioning

confidence: 99%

Novel Perspectives towards RNA-Based Nano-Theranostic Approaches for Cancer Management

et al. 2021

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In the fight against cancer, early diagnosis is critical for effective treatment. Traditional cancer diagnostic technologies, on the other hand, have limitations that make early detection difficult. Therefore, multi-functionalized nanoparticles (NPs) and nano-biosensors have revolutionized the era of cancer diagnosis and treatment for targeted action via attaching specified and biocompatible ligands to target the tissues, which are highly over-expressed in certain types of cancers. Advancements in multi-functionalized NPs can be achieved via modifying molecular genetics to develop personalized and targeted treatments based on RNA interference. Modification in RNA therapies utilized small RNA subunits in the form of small interfering RNAs (siRNA) for overexpressing the specific genes of, most commonly, breast, colon, gastric, cervical, and hepatocellular cancer. RNA-conjugated nanomaterials appear to be the gold standard for preventing various malignant tumors through focused diagnosis and delivering to a specific tissue, resulting in cancer cells going into programmed death. The latest advances in RNA nanotechnology applications for cancer diagnosis and treatment are summarized in this review.

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DNA Nanobiosensors: An Outlook on Signal Readout Strategies

Cited by 23 publications

References 92 publications

Advancements in Testing Strategies for COVID-19

Advancements in Testing Strategies for COVID-19

Aptamers for Viral Detection and Inhibition

Novel Perspectives towards RNA-Based Nano-Theranostic Approaches for Cancer Management

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