Abstract:Advances in peroxidase and biotin ligase-mediated signal
amplification
have enabled high-resolution subcellular mapping of endogenous RNA
localization and protein–protein interactions. Application
of these technologies has been limited to RNA and proteins because
of the reactive groups required for biotinylation in each context.
Here we report several novel methods for proximity biotinylation of
exogenous oligodeoxyribonucleotides by application of well-established
and convenient enzymatic tools. We describe a… Show more
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