DNA mini-barcoding: An approach for forensic identification of some endangered Indian snake species

Dubey, Bhawna; Meganathan, P. R.; Haque, Ikramul

doi:10.1016/j.fsigen.2010.03.001

Cited by 52 publications

(33 citation statements)

References 27 publications

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“…Besides the evolutionary perspective, the polymorphisms in the mitochondrial genomes have been utilized for various ecological studies (Avise, 1995;Valentini et al, 2009). The forensic utility is another aspect where the mitochondrial genome sequences have proved useful for species identification and thereby are a valuable tool for the conservation of endangered species (Teletchea et al, 2005;Dubey et al, 2009Dubey et al, , 2010Meganathan et al, 2009aMeganathan et al, , 2010a. Thus, the generation of mitochondrial genome sequences is useful for phylogenetic analyses and also for informing conservation efforts.…”

Section: Introductionmentioning

confidence: 99%

Complete mitochondrial genome sequences of three Crocodylus species and their comparison within the Order Crocodylia

Meganathan

Dubey

Batzer

et al. 2011

Gene

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Section: Introductionmentioning

confidence: 99%

Complete mitochondrial genome sequences of three Crocodylus species and their comparison within the Order Crocodylia

Meganathan

Dubey

Batzer

et al. 2011

Gene

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“…In this regard, molecular techniques have proven to be an effective tool for reliable species identification, phylogenetics, and biodiversity analysis, and may be the key to successful work in law enforcement and conservation of wildlife [17,18]. Phylogenetics and phylogeographic studies of snakes are rare and most have used mitochondrial genes, such as cytochrome c oxidase subunit 1 (CO1), cytochrome b (Cyt b), and NADH dehydrogenase subunit 4 (ND4) [9,19,20].…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of the king cobra, Ophiophagus hannah in Thailand based on mitochondrial DNA sequences

Suntrarachun

Chanhome

Sumontha³

2014

Asian Biomedicine

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“…Firstly, the percentage similarity between the NCBI reference and query sequence were required to be high. A threshold value of >98% was used for Cytb and 12S genes, a similar value has been employed in DNA barcoding approaches using COI [26]. Although the 98% cut-off is somewhat imprecise, such limits are required unless a detailed knowledge of the inter-specific and intra-specific variation exists for each mtDNA gene.…”

Section: Assignment Of Species Identity Using Genetic Datamentioning

confidence: 99%

Egg forensics: An appraisal of DNA sequencing to assist in species identification of illegally smuggled eggs

Coghlan

White

Parkinson

et al. 2012

Forensic Science International: Genetics

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Psittaciformes (parrots and cockatoos) are charismatic birds, their plumage and capacity for learning make them highly sought after pets. The illegal trade in parrots and cockatoos poses a serious threat to the viability of native populations; in addition, species transported to non-endemic areas may potentially vector disease and genetically 'pollute' local native avifauna. To reduce the logistical difficulties associated with trafficking live birds, smugglers often transport eggs. This creates a problem for authorities in elucidating accurate species identification without the laborious task of incubation and hand rearing until a morphological identification can be made. Here, we use 99 avian eggs seized from carriers coming into and within Australia, as a result of suspected illegal trade. We investigate and evaluate the use of mitochondrial DNA (mtDNA) to accurately identify eggs to family, genus or species level. However, Identification of a species based on percentage mtDNA similarities is difficult without good representations of the inter-and intra-levels of species variation.Based on the available reference database, we were able to identify 52% of the eggs to species level.Of those, 10 species from eight genera were detected, all of which belong to the parrot (Psittacidae) and cockatoo (Cacatuidae) families. Of the remaining 48%, a further 36% of eggs were identified to genus level, and 12% identified to family level using our assignment criteria. Clearly the lack of validated DNA reference sequences is hindering our ability to accurately assign a species identity, and accordingly, we advocate that more attention needs to be paid to establishing validated, multi locus mtDNA reference databases for exotic birds that can both assist in genetic identifications and withstand legal scrutiny.

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DNA mini-barcoding: An approach for forensic identification of some endangered Indian snake species

Cited by 52 publications

References 27 publications

Complete mitochondrial genome sequences of three Crocodylus species and their comparison within the Order Crocodylia

Complete mitochondrial genome sequences of three Crocodylus species and their comparison within the Order Crocodylia

Phylogenetic analysis of the king cobra, Ophiophagus hannah in Thailand based on mitochondrial DNA sequences

Egg forensics: An appraisal of DNA sequencing to assist in species identification of illegally smuggled eggs

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