DNA‐Mikroarrays als Decodierungswerkzeuge in der kombinatorischen Chemie und der chemischen Biologie

Lovrinovic, Marina; Niemeyer, Christof M.

doi:10.1002/ange.200500645

Cited by 11 publications

(8 citation statements)

References 40 publications

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“…[275] A further case in point is the combination of multiple orthogonal DNA assembly processes with the stable biotin/ SAv template, which avoids laborious DNA labeling of proteins. [243,255,[276][277][278][279] In several studies, reviewed elsewhere, [16,280,281] Niemeyer and co-workers employed the complexation of biotinylated proteins with SAv-DNA to form protein-oligonucleotide preconjugates (Figure 11). These preconjugates were then immobilized onto a surface displaying complementary oligonucleotides.…”

Section: Dna-modified Surfacesmentioning

confidence: 99%

Chemical Strategies for Generating Protein Biochips

et al. 2008

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Protein biochips are at the heart of many medical and bioanalytical applications. Increasing interest has been focused on surface activation and subsequent functionalization strategies for immobilizing these biomolecules. Different approaches using covalent and noncovalent chemistry are reviewed; particular emphasis is placed on the chemical specificity of protein attachment and on retention of protein function. Strategies for creating protein patterns (as opposed to protein arrays) are also outlined. An outlook on promising and challenging future directions for protein biochip research and applications is also offered.

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Section: Dna-modified Surfacesmentioning

confidence: 99%

Chemical Strategies for Generating Protein Biochips

et al. 2008

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“…[24] Recent applications of the DDI principle have demonstrated that DNA microarrays can be used efficiently as decoding tools in combinatorial chemistry and chemical biology. [25] In addition to this field of research, the heterogeneous hybridization implemented in DDI is also important for DNA computing on surfaces (see Section 6.1). The hybridization of short complementary DNA oligonucleotides can also be utilized for the assembly of metal and semiconductor nanoparticles tagged with appropriate DNA oligomers.…”

Section: General Considerations Of Dna-sequence Designmentioning

confidence: 99%

“…In the early 1980s, Seeman conducted pioneering work on the construction of artificial nucleic acid architectures using synthetic DNA branched junction motifs mainly containing three and four arms (motifs 1 and 2 in Figure 4), [37] which are schematic representation of DNA-based directional assembly of four different nanoscale building blocks to form a stoichiometrically and spatially defined supramolecular aggregate. [28] The upper section was reprinted with permission from reference [25].…”

Section: Junctions and Latticesmentioning

confidence: 99%

Rational Design of DNA Nanoarchitectures

2006

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DNA has many physical and chemical properties that make it a powerful material for molecular constructions at the nanometer length scale. In particular, its ability to form duplexes and other secondary structures through predictable nucleotide-sequence-directed hybridization allows for the design of programmable structural motifs which can self-assemble to form large supramolecular arrays, scaffolds, and even mechanical and logical nanodevices. Despite the large variety of structural motifs used as building blocks in the programmed assembly of supramolecular DNA nanoarchitectures, the various modules share underlying principles in terms of the design of their hierarchical configuration and the implemented nucleotide sequences. This Review is intended to provide an overview of this fascinating and rapidly growing field of research from the structural design point of view.

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“…5, 6 Motivated by the extraordinary performance of the DNA‐directed immobilization (DDI) of proteins and other molecular and colloidal components,7 we herein describe that micropatterns of cell‐surface ligands can be generated on DNA microarrays by the DDI method and that the resulting surfaces are suitable substrates for the growth of fibroblast cells (Figure 1). This approach should have significant advantages over conventional spotting procedures because it would enable the implementation of the power of DNA microarrays as decoding tools in combinatorial synthesis and screening of ligands8 into cellular biology research. To experimentally investigate this hypothesis, we chose the well‐studied recognition of RGD peptide ligands by integrin surface receptors of fibroblast cells as the model system 9–11…”

mentioning

confidence: 99%

Generation of Live‐Cell Microarrays by Means of DNA‐Directed Immobilization of Specific Cell‐Surface Ligands

et al. 2007

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Microarray technology has become one of the principal platform technologies for the high-throughput analysis of biological systems.[1] Starting with the evolution of DNA microarrays in the 1990s, the developments of peptide and protein microarrays to elucidate interaction partners, modification sites, and enzyme substrates, [2,3] this technology is nowadays moving towards the construction of microarrays of fixed-tissue samples [4] and live cells. [5] The latter are expected to help unravel complex cellular traits in both healthy and diseased states because cell microarrays provide the ability to molecularly delineate the characteristics of individual cells from complex mixtures. By immobilizing different cellcapture and analysis reagents on a solid support, mixtures of cells can be rapidly interrogated for their composition and phenotype, thus helping to identify and quantitate distinct cell types based on the expression of particular cell-surface molecules. Furthermore, this immobilization helps to analyze the response of different cell types to defined signals through the secretion of specific factors or other measurable cellular activities. [5] To immobilize cell-capture moieties on suitable solid supports, proteins, peptides, or other ligands are usually attached to surfaces by using covalent coupling, chemisorption, or physisorption processes in combination with robotic spotting procedures. [1,5,6] Motivated by the extraordinary performance of the DNA-directed immobilization (DDI) of proteins and other molecular and colloidal components, [7] we herein describe that micropatterns of cell-surface ligands can be generated on DNA microarrays by the DDI method and that the resulting surfaces are suitable substrates for the growth of fibroblast cells (Figure 1). This approach should have significant advantages over conventional spotting procedures because it would enable the implementation of the power of DNA microarrays as decoding tools in combinatorial synthesis and screening of ligands [8] into cellular biology research. To experimentally investigate this hypothesis, we chose the well-studied recognition of RGD peptide ligands by integrin surface receptors of fibroblast cells as the model system. [9][10][11] As depicted in Figure 1, covalent conjugates of streptavidin (STV) and single-stranded DNA (ssDNA), were used as molecular adaptors in the DNA-directed immobilization of biotinylated peptides. To this end, three different DNA-STV conjugates (F1, F5, F10, shown schematically in Figure 1) containing the 22-mer oligonucleotides tF1, tF5, and tF10, respectively, were prepared as previously described. [12,13] In separate reaction tubes, conjugates F1 and F5 were then coupled with one molar equivalent of the biotinylated peptide bRGDF (biotin-Gly 5 -Arg-Gly-Asp-Phe-COOH), [10] and, as a control, bG5 (biotin-Gly 5 -COOH), thus leading to conjugates F1-bRGDF and F5-bG5, respectively. To allow for visualization of the immobilized conjugates by fluorescence analysis, five molar equivalents of biotinylated fluorescen...

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DNA‐Mikroarrays als Decodierungswerkzeuge in der kombinatorischen Chemie und der chemischen Biologie

Cited by 11 publications

References 40 publications

Chemical Strategies for Generating Protein Biochips

Chemical Strategies for Generating Protein Biochips

Rational Design of DNA Nanoarchitectures

Generation of Live‐Cell Microarrays by Means of DNA‐Directed Immobilization of Specific Cell‐Surface Ligands

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