DNA microdevice for electrochemical detection of Escherichia coli 0157:H7 molecular markers

Berganza, J.; Olabarría, Garbiñe; Garcia, Regina Conceição; Verdoy, Dolores; Rebollo, Amaia; Arana, Sergio

doi:10.1016/j.bios.2006.09.028

Cited by 40 publications

(19 citation statements)

References 38 publications

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“…On the other hand, the electrodes pre-treated with perchloric acid had an ESA of 2.83 × 10 −2 ± 0.9 × 10 −3 cm 2 (mean value and standard deviation of five electrodes) and a roughness factor (R f ) of 1.41 ± 0.05. These results improve the values obtained by other research groups (Bonroy et al, 2004;Carvalhal et al, 2005) with high reproducibility and confirm the increase in the active area that provides the gold electrodes with better biosensing features.…”

Section: Influence Of the Electropolishing Solutionsupporting

confidence: 88%

“…These devices allow realtime analysis of biological reactions and can be applied to nearly any field. This kind of sensors, fabricated by means of microsystem techniques, are analytical devices that consist of two main components: a bioreceptor and a transducer that turns the detection event into an electrical output which is instantly measured (Berganza et al, 2007). The integration of the three electrodes and the use of thin-film technologies provide the developed system with the miniaturization and portability needed to carry out field experiments while decreases both the fabrication and operation costs.…”

Section: Introductionmentioning

confidence: 99%

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Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

Zuzuarregui

Arana

Pérez-Lorenzo

et al. 2013

J. Sens. Sens. Syst.

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Abstract. In this paper an electrochemical endotoxin biosensor consisting of an immobilized lipopolysaccharide (LPS) ligand, polymyxin B (PmB), is presented. Several parameters involved both in the device fabrication and in the detection process were analyzed to optimize the ligand immobilization and the interaction between PmB and LPS, aiming at increasing the sensitivity of the sensor. Different electrochemical pre-treatment procedures as well as the functionalization methods were studied and evaluated. The use of a SAM (self-assembled monolayer) to immobilize PmB and the quantification of the interactions via cyclic voltammetry allowed the development of a robust and simple device for in situ detection of LPS. Thus, the biosensor proposed in this work intends an approach to the demanding needs of the market for an integrated, portable and simple instrument for endotoxin detection.

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Section: Influence Of the Electropolishing Solutionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

Zuzuarregui

Arana

Pérez-Lorenzo

et al. 2013

J. Sens. Sens. Syst.

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“…However, many of these methods are so time-consuming, expensive or complicated that they are not suitable for fast detection of E. coli O157:H7. As alternative to the conventional methods, biosensors have been explored for pathogenic bacteria detection in recent years (Berganza et al, 2007;Berkenpas et al, 2006;Varshney and Li, 2007). Advantages of this approach include its continuous data acquisition ability, target specificity, fast response, mass produce feasibility and sample preparation simplicity.…”

Section: Introductionmentioning

confidence: 99%

Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor

Wang

et al. 2008

J. Zhejiang Univ. Sci. B

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By means of the specific immuno-recognition and ultra-sensitive mass detection, a quartz crystal microbalance (QCM) biosensor for Escherichia coli O157:H7 detection was developed in this work. As a suitable surfactant, 16-mercaptohexadecanoic acid (MHDA) was introduced onto the Au surface of QCM, and then self-assembled with N-hydroxysuccinimide (NHS) raster as a reactive intermediate to provide an active interface for the specific antibody immobilization. The binding of target bacteria with the immobilized antibodies decreased the sensor's resonant frequency, and the frequency shift was correlated to the bacterial concentration. The stepwise assembly of the immunosensor was characterized by means of the electrochemical techniques. Using the immersion-dry-immersion procedure, this QCM biosensor could detect 2.0×10 2 colony forming units (CFU)/ml E. coli O157:H7. In order to reduce the fabrication time, a polyelectrolyte layer-by-layer self-assembly (LBL-SA) method was adopted for fast construction. Finally, the reproducibility of this biosensor was discussed.

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“…For this purpose, several techniques have been investigated based on recognition of DNA hybridization, using electroactive labels, dye molecules, nanoparticles, or label free methods. The coupling of DNA biosensors with PCR amplification reaction is a recent field of research and provides genetic identification of selected species [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Response Surface Methodology for Optimization of Food Borne Pathogen Detection in Real Samples Based on Label Free Electrochemical Nucleic Acid Biosensors

et al. 2011

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The response surface methodology (RSM) is used for optimization of foodborne pathogen detection based on label free electrochemical nucleic acid biosensors. Listeria monocytogenes amplicons obtained from food samples are used as model case. The extent of hybridization is determined by using guanine oxidation signals obtained with Differential Pulse Voltammetry and Electrochemical Impedance Spectroscopy. RSM is used to investigate the effects of hybridization parameters including target and salt concentration and hybridization time on the biosensor selectivity. The ratio of electrochemical transductions after hybridization with complementary and noncomplementary targets is the response of the statistical analysis obtaining a detection limit of 267 pM.

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DNA microdevice for electrochemical detection of Escherichia coli 0157:H7 molecular markers

Cited by 40 publications

References 38 publications

Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor

Response Surface Methodology for Optimization of Food Borne Pathogen Detection in Real Samples Based on Label Free Electrochemical Nucleic Acid Biosensors

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