DNA Microarray Expression Analysis of Baicalin‐Induced Differentiation of C17.2 Neural Stem Cells

Li, Ming; Choi, Sze-Ting; Tsang, K. S.; Shaw, Pang‐Chui; Lau, Kwok‐Fai

doi:10.1002/cbic.201200145

Cited by 8 publications

(6 citation statements)

References 40 publications

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“…A few studies have reported the differentiation potential of some herbal extracts on different types of stem cells (Udalamaththa et al, 2016). Additionally, several plant extracts and their active components showed potential effects in neuronal differentiation and proliferation, like phytochemicals from Butea superba in stem cells (Arai et al, 2013), flavonoid component from Scutellaria baicalensis in C17.2 cells (Li et al, 2012), acetic acid extract of Mucuna gigantea in bone marrow-derived hMSCs (Kongros et al, 2012), Salvia miltiorrhiza extract on Wharton jelly derived hMSCs (Ma et al, 2005). However, to our knowledge, there is no study to date reporting the effect of any natural compounds on hAECs differentiation.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Potential Role of Rosmarinic Acid in Neuronal Differentiation of Human Amnion Epithelial Cells by Microarray Gene Expression Profiling

et al. 2019

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In the present study, we conducted microarray gene expression profiling to explore the direction of differentiation of human amnion epithelial cells (hAECs) treated with rosmarinic acid (RA). hAECs have several clinical advantages over other types of stem cells, including availability, low immunogenicity, low rejection rate, non-tumorigenicity, and less ethical constraint. On the other hand, RA is a phenolic compound having several health benefits, including neuroprotective and antidepressant-like activities. In this study, hAECs were isolated from discarded term placenta and were treated with 20 μM RA for 7 days. Microarray gene expression profiling was conducted for three biological replicates of RA-treated and untreated control cells on day 0 and day 7. Gene set enrichment analysis, and gene annotation and pathway analysis were conducted using online data mining tools GSEA and DAVID. Gene expression profiling showed that RA treatment biased hAECs toward ectodermal lineage progression, regulated transcription factors involved in neuronal differentiation, regulated neural specific epigenetic modifiers and several extracellular signaling pathways of neural induction, and significantly inhibited Notch signaling pathway. Gene expression profiling of RA-treated hAECs reveals for the first time a potential role of RA in neural induction and neuronal differentiation of hAECs. Having a naturally occurring compound as differentiation inducer as well as a readily available source of stem cells would have great advantages for the cell-based therapies. Findings from our genome-wide analysis could provide a foundation for further in-depth investigation.

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Section: Introductionmentioning

confidence: 99%

Exploring the Potential Role of Rosmarinic Acid in Neuronal Differentiation of Human Amnion Epithelial Cells by Microarray Gene Expression Profiling

et al. 2019

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“…Baicalin is a flavonoid found in the Chinese herb Scutellaria baicalensis. Previous studies had demonstrated that baicalin could promote the neural differentiation of human iPS cells, immortalized mouse C17.2 neural progenitor cells, and rat embryonic cortical NSCs [11][12][13]. Interestingly, baicalin improved neural differentiation but inhibited glial formation [12].…”

Section: Neural Differentiationmentioning

confidence: 97%

“…Previous studies had demonstrated that baicalin could promote the neural differentiation of human iPS cells, immortalized mouse C17.2 neural progenitor cells, and rat embryonic cortical NSCs [11][12][13]. Interestingly, baicalin improved neural differentiation but inhibited glial formation [12]. Many other traditional Chinese medical herbal ingredients also have the ability to differentiate stem cells into specialized cell subtypes.…”

Section: Neural Differentiationmentioning

confidence: 99%

Effect of Active Ingredients of Chinese Herbal Medicine on the Rejuvenation of Healthy Aging: Focus on Stem Cells

Wang

Ling

2020

Evidence-Based Complementary and Alternative Medicine

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Stem cells (SCs) are special types of cells with the ability of self-renewal and multidirectional differentiation. As the organism ages, the ability to maintain homeostasis and regeneration deteriorates and the number and activity of stem cells decline. Theoretically, the restoration of stem cells might reverse aging. However, due to their own aging, donor-derived immune rejection, and difficulties in stem cell differentiation control, a series of problems need to be solved to realize the potential for clinical application of stem cells. Chinese herbal medicine is a nature drug library which is suitable for the long-term treatment of aging-related diseases. Modern pharmacological studies have revealed that many active ingredients of Chinese herbal medicines with the effect of promoting stem cells growth and differentiation mainly belong to “reinforcing herbs.” In recent years, exploration of natural active ingredients from Chinese herbal medicines for delaying aging, improving the stem cell microenvironment, and promoting the proliferation and differentiation of endogenous stem cells has attracted substantial attention. This article will focus on active ingredients from Chinese herbs-mediated differentiation of stem cells into particular cell type, like neural cells, endothelial cells, cardiomyocytes, and osteoblasts. We will also discuss the effects of these small molecules on Wnt, Sonic Hedgehog, Notch, eNOS-cGMP, and MAP kinase signal transduction pathways, as well as reveal the role of estrogen receptor α and PPAR γ on selectively promoting or inhibiting stem cells differentiation. This review will provide new insights into the health aging strategies of active ingredients in Chinese herbal medicine in regenerative medicine.

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“…Baicalin can stimulate differentiation of C17.2 NSCs by activating Erk1/2 (Li et al, 2011), which controls neural differentiation-related gene expression. Under the effects of Baicalin, the expression levels of NeuroD2, Ngn1, and Ngn2 mRNA are upregulated (Li M. et al, 2012) (Figure 4). Neurogenic , 2019).…”

Section: Baicalin and Neural Stem Cellsmentioning

confidence: 99%

Baicalin regulates stem cells as a creative point in the treatment of climacteric syndrome

et al. 2022

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DNA Microarray Expression Analysis of Baicalin‐Induced Differentiation of C17.2 Neural Stem Cells

Cited by 8 publications

References 40 publications

Exploring the Potential Role of Rosmarinic Acid in Neuronal Differentiation of Human Amnion Epithelial Cells by Microarray Gene Expression Profiling

Exploring the Potential Role of Rosmarinic Acid in Neuronal Differentiation of Human Amnion Epithelial Cells by Microarray Gene Expression Profiling

Effect of Active Ingredients of Chinese Herbal Medicine on the Rejuvenation of Healthy Aging: Focus on Stem Cells

Baicalin regulates stem cells as a creative point in the treatment of climacteric syndrome

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