DNA methyltransferases have an essential role in female fecundity in brown planthopper, Nilaparvata lugens

Jiao, Zhenbiao; Xing, Yao‐Wu; Li, Yao; Yin, Chuanlin; Ge, Chutian; Li, Fēi

doi:10.1016/j.bbrc.2015.05.114

Cited by 45 publications

(38 citation statements)

References 37 publications

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“…Our results, when coupled with those of prior investigations of arthropod Dnmt expression (Zwier et al, 2012;Yang et al, 2014;Mitsudome et al, 2015;Zhang et al, 2015;Robinson et al, 2016), reveal several commonalities among diverse arthropod taxa. In particular, extensive patterning of DNA methylation, as inferred by elevated Dnmt expression, apparently occurs in germ cells and embryos (Table 2).…”

Section: Discussionsupporting

confidence: 79%

“…This suggests that DNMT1 functions primarily as a maintenance methyltransferase in B. mori, despite a putative secondary role in de novo methylation. Zwier et al (2012) Migratory locust, Locusta migratoria DNMT1 expression elevated in ovaries and testes relative to embryos and heads Robinson et al (2016) Brown planthopper, Nilaparvata lugens DNMT3 expression elevated in gravid females relative to other stages and males Zhang et al (2015) Two-spotted spider mite, Tetranychus urticae DNMT3 expression elevated in embryos and adult males relative to other stages; in situ hybridization consistent with elevated expression in ovaries and testes relative to other tissues Yang et al (2014) Our expression results in S. invicta are consistent with the presumed de novo function of DNMT3 and maintenance function of DNMT1. In particular, Dnmt3 was most highly expressed during embryonic development in S. invicta (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Developmental DNA methyltransferase expression in the fire ant Solenopsis invicta

2017

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DNA methylation is accomplished in animals by 2 classes of enzymes known as DNA methyltransferases, DNMT3 and DNMT1, which perform de novo methylation and maintenance methylation, respectively. Several studies of hymenopteran eusocial insects suggest that DNA methylation is capable of influencing developmental plasticity. However, fundamental questions remain about the patterning of DNA methylation during the course of insect development. In this study, we performed quantitative real-time PCR (qPCR) on transcripts from the single-copy orthologs of DNMT1 and DNMT3 in the red imported fire ant, Solenopsis invicta. In particular, we assessed the expression of S. invicta Dnmt1 and Dnmt3 mRNA during 7 stages of worker development, among behaviorally distinct adults, and among male and female gonads. Dnmt3 was most highly expressed during embryonic development, whereas Dnmt1 was similarly expressed throughout the course of development. Moreover, Dnmt1 and Dnmt3 were highly expressed in testes and ovaries. Neither Dnmt was significantly differentially expressed among heads of behaviorally distinct adult castes. Our results support the hypothesis that extensive patterning of DNA methylation occurs during gametogenesis and embryogenesis in the insect order Hymenoptera.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Developmental DNA methyltransferase expression in the fire ant Solenopsis invicta

2017

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“…Until now, similar results were only reported for DNMT1 and DNMT3. For example, for the brown plant hopper, Nilaparvata lugens, it was recently reported that the knockdown of DNMT1 and DNMT3 reduced the number of offspring by suppressing ovary development (Zhang et al, 2015). Further, RNAi-mediated silencing was also used to demonstrate that DNMT1 is essential for egg production and embryo viability in the large milkweed bug, Oncopeltus fasciatus (Bewick et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Silencing of the DNA methyltransferase 1 associated protein 1 (DMAP1) gene in the invasive ladybird Harmonia axyridis implies a role of the DNA methyltransferase 1‐DMAP1 complex in female fecundity

Gegner

Vogel

et al. 2019

Insect Molecular Biology

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The invasive harlequin ladybird Harmonia axyridis is a textbook example of polymorphism and polyphenism as the temperature during egg development determines the frequency of melanic morphs and the number and size of black spots in nonmelanic morphs. Recent concepts in evolutionary biology suggest that epigenetic mechanisms can translate environmental stimuli into heritable phenotypic changes. To investigate whether epigenetic mechanisms influence the penetrance and expressivity of colour morphs in H. axyridis, we used RNA interference to silence key enzymes required for DNA methylation and histone modification. We found that neither of these epigenetic mechanisms affected the frequency of different morphs, but there was a significant impact on life‐history traits such as longevity and fecundity. Strikingly, we found that silencing the gene encoding for DNA methyltransferase 1 associated protein 1 (DMAP1) severely reduced female fecundity, which correlated with an abundance of degenerated ovaries in DMAP1‐knockdown female beetles. Finally, we observed significant differences in DMAP1 expression when we compared native and invasive H. axyridis populations with a biocontrol strain differing in egg‐laying capacity, suggesting that the DNA methyltransferase 1‐DMAP1 complex may influence the invasive performance of this ladybird.

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“…DNA methylation is one of the insect epigenetics that has received extensive attention (Jaenisch and Bird, ; Field et al , ; Lyko and Maleszka, ; Mandrioli and Manicardi, ). BPH females with mutation in either of the DNA methyltransferase genes ( Dnmt1 or Dnmt3 ) produce fewer offspring (Zhang et al , ). The function of MTase15 characterized in this study adds an additional twist in the complex regulatory network of the IIS pathway and epigenetic change on BPH fertility.…”

Section: Discussionmentioning

confidence: 99%

The MTase15 regulates reproduction in the wing‐dimorphic planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

Chen

Xue

et al. 2019

Insect Molecular Biology

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S‐Adenosyl‐l‐methionine‐dependent methyltransferases (SAMMTases) modulate important cellular and metabolic activities in both prokaryotes and eukaryotes. Here, we functionally characterized an SAMMTase gene (MTase15) in the migratory brown planthopper (BPH), Nilaparvata lugens, which is the most notorious rice pest in Asia. The cDNA sequence of MTase15 is 2764 nt in length with an open reading frame of 1218 nt encoding 405 amino acid residues. Quantitative real‐time PCR analysis showed that MTase15 was readily detected from egg to adult stages and extensively distributed in various body parts of adult females and males, with slightly high levels in ovary and testis, respectively. In addition, MTase15 was transcriptionally regulated by the insulin signalling pathway in BPH. RNA‐interference‐mediated knockdown of MTase15 (dsMtase15) resulted in deficiencies in vitellogenin synthesis and oogenesis, and female infertility. Males with Mtase15 knockdown retained the capability of producing sperms with normal viability, but less sperm was transferred to wild‐type (wt) females during copulation, and eggs laid by these wt females arrested embryogenesis. These findings not only assign a functional role to MTase15, but also provide a link between the insulin signalling pathway and epigenetic regulation in BPH reproduction.

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DNA methyltransferases have an essential role in female fecundity in brown planthopper, Nilaparvata lugens

Cited by 45 publications

References 37 publications

Developmental DNA methyltransferase expression in the fire ant Solenopsis invicta

Developmental DNA methyltransferase expression in the fire ant Solenopsis invicta

Silencing of the DNA methyltransferase 1 associated protein 1 (DMAP1) gene in the invasive ladybird Harmonia axyridis implies a role of the DNA methyltransferase 1‐DMAP1 complex in female fecundity

The MTase15 regulates reproduction in the wing‐dimorphic planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

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