2014
DOI: 10.1038/bjc.2014.337
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DNA methylation status is more reliable than gene expression at detecting cancer in prostate biopsy

Abstract: Background:We analysed critically the potential usefulness of RNA- and DNA-based biomarkers in supporting conventional histological diagnostic tests for prostate carcinoma (PCa) detection.Methods:Microarray profiling of gene expression and DNA methylation was performed on 16 benign prostatic hyperplasia (BPH) and 32 cancerous and non-cancerous prostate samples extracted by radical prostatectomy. The predictive value of the selected biomarkers was validated by qPCR-based methods using tissue samples extracted f… Show more

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Cited by 68 publications
(56 citation statements)
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References 48 publications
(61 reference statements)
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“…Although ConfirmMDx aims mainly at identifying significant PCa by improving sensitivity of detection, DNA methylation assays might also be a valuable tool to distinguish PCa from BPH. In the study by Paziewska et al [85], DNA methylation levels of APC, TACC2, RARB, DGKZ and HES5 promoter regions provided an excellent discrimination between malignant and benign tissues with AUC reaching 0.95-1. Interestingly, in the same study, standard analysis of gene expression of the best 3 genes (HOXC6, AMACR, and PCA3) yielded only moderate accuracy with AUC >0.8 [85].…”
Section: Reviewmentioning
confidence: 93%
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“…Although ConfirmMDx aims mainly at identifying significant PCa by improving sensitivity of detection, DNA methylation assays might also be a valuable tool to distinguish PCa from BPH. In the study by Paziewska et al [85], DNA methylation levels of APC, TACC2, RARB, DGKZ and HES5 promoter regions provided an excellent discrimination between malignant and benign tissues with AUC reaching 0.95-1. Interestingly, in the same study, standard analysis of gene expression of the best 3 genes (HOXC6, AMACR, and PCA3) yielded only moderate accuracy with AUC >0.8 [85].…”
Section: Reviewmentioning
confidence: 93%
“…In the study by Paziewska et al [85], DNA methylation levels of APC, TACC2, RARB, DGKZ and HES5 promoter regions provided an excellent discrimination between malignant and benign tissues with AUC reaching 0.95-1. Interestingly, in the same study, standard analysis of gene expression of the best 3 genes (HOXC6, AMACR, and PCA3) yielded only moderate accuracy with AUC >0.8 [85]. Simultaneously with growing interest in invasive epigenetic assays, non-invasive methylation biomarkers were evaluated.…”
Section: Reviewmentioning
confidence: 93%
“…Today, a new generation of biomarkers is emerging, consisting of serum-, urine-, and tissue-based assays that may supplement PSA testing, or replace it over time [3]. Diagnostic biomarkers should be sensitive and specific enough to show whether disease is present; however, the levels of most biomarkers overlap between tumor and normal tissues [4]. Consequently, it remains difficult to identify an ideal biomarker with enough discriminatory power for an accurate diagnosis of prostate cancer [4].…”
mentioning
confidence: 99%
“…Diagnostic biomarkers should be sensitive and specific enough to show whether disease is present; however, the levels of most biomarkers overlap between tumor and normal tissues [4]. Consequently, it remains difficult to identify an ideal biomarker with enough discriminatory power for an accurate diagnosis of prostate cancer [4]. Histological evaluation remains the gold standard.…”
mentioning
confidence: 99%
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