DNA Methylation Profiling of hTERT Gene Alongside with the Telomere Performance in Gastric Adenocarcinoma

Vahidi, Sogand; Norollahi, Seyedeh Elham; Agah, Shahram; Samadani, Ali Akbar

doi:10.1007/s12029-020-00427-7

Cited by 9 publications

(3 citation statements)

References 90 publications

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“…of immortalization, such as simian virus 40 (SV40) or hTERT [16] . However, compared with primary cells, SV40 transfection may induce cell chromosomal abnormalities, cell cycle control changes and increased carcinogenic risk [17] . Some studies have confirmed that hTERT transfection does not cause malignant transformation of cells, and does not change cell characteristics and karyotype stability [18,19] .…”

Section: Immortalizing Cells By Introducing Foreign Genes Into Cells Through Viruses Is a Commonly Used Methodsmentioning

confidence: 99%

Immortalization of human dermal microvascular endothelial cells

Wang¹,

Zhang²,

Feng³

et al. 2021

Preprint

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Objectives : Microvascular endothelial cells (MECs) have been proved by increasing studies to play important roles in the process of endocrine, immune response, and pathogenic microorganism infection. However, most types of MECs have a limited number of divisions. Therefore, the immortalization of primary MECs may provide a better cell model for research. And the present research is aimed to establish an immortal human dermal microvascular endothelial cells (HDMECs). Methods : To immortalize HDMECs, the telomerase reverse transcriptase (hTERT) gene was transferred into the primary HDMECs by lentiviral infection. The passages of HDMECs transfected with hTERT or without hTERT were analyzed. At the same time, the relative telomerase activity and telomere length in HDMECs transfected with hTERT were detected by RT-PCR assay. And the β-galactosidase (β-GAL) activity in HDMECs transfected with hTERT was detected by ELISA kits. Finally, karyotype and tube formation analysis were used to evaluate the effects of transfection with hTERT on the characteristics of HDMECs. Results: The results showed that the number of passages of HDMECs transfected with hTERT was significantly increased. The telomerase activity of HDMECs transfected with hTERT gene was enhanced, and β-GAL activity was significantly reduced. Moreover, the transfection of hTERT gene has almost no effect on the karyotype and tube formation of HDMECs. Conclusion: These data indicate that transfection of hTERT gene could successfully enhance the cleavage ability of HDMECs, and the characteristics of hTERT-HDMECs remain almost unchanged.

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Section: Immortalizing Cells By Introducing Foreign Genes Into Cells Through Viruses Is a Commonly Used Methodsmentioning

confidence: 99%

Immortalization of human dermal microvascular endothelial cells

Wang¹,

Zhang²,

Feng³

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…The nuclear distribution of lcnRNAs and their interaction with a several nuclear elements, such as transcription factors, chromatin remodeling complexes, or even with a DNA establishing a DNA-RNA complex, have revealed their importance in the regulation of both gene transcription and its epigenetic landscape [ 31 , 32 , 33 ]. Most types of cancer require numerous changes in nuclear transcription homeostasis in order to escape cellular control systems.…”

Section: Genetic and Epigenetic Contributions Of Lncrna Dysregulation...mentioning

confidence: 99%

Molecular Mechanisms of lncRNAs in the Dependent Regulation of Cancer and Their Potential Therapeutic Use

García-Padilla

Dueñas

García‐López

et al. 2022

IJMS

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Deep whole genome and transcriptome sequencing have highlighted the importance of an emerging class of non-coding RNA longer than 200 nucleotides (i.e., long non-coding RNAs (lncRNAs)) that are involved in multiple cellular processes such as cell differentiation, embryonic development, and tissue homeostasis. Cancer is a prime example derived from a loss of homeostasis, primarily caused by genetic alterations both in the genomic and epigenetic landscape, which results in deregulation of the gene networks. Deregulation of the expression of many lncRNAs in samples, tissues or patients has been pointed out as a molecular regulator in carcinogenesis, with them acting as oncogenes or tumor suppressor genes. Herein, we summarize the distinct molecular regulatory mechanisms described in literature in which lncRNAs modulate carcinogenesis, emphasizing epigenetic and genetic alterations in particular. Furthermore, we also reviewed the current strategies used to block lncRNA oncogenic functions and their usefulness as potential therapeutic targets in several carcinomas.

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“…Tumor suppressor gene inhibition interrupts the normal cell cycle equilibrium, potentially leading to increased cell reproduction and cancer. Hypermethylation of unmethylated tumor suppressor gene promoter areas, interrupts the gene by inhibiting transcription and the capacity to control aberrant cell reproduction, therefore driving cancerous transformation [ 28 , 29 ].…”

Section: The Function Of Dna Methylation In Carcinogenesismentioning

confidence: 99%

Performance of DNA Methylation on the Molecular Pathogenesis of Helicobacter pylori in Gastric Cancer; targeted therapy approach

et al. 2022

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Gastric cancer (GC) is a significant cause of cancer mortality which has led to focused exploration of the pathology of GC. The advent of genome-wide analysis methods has made it possible to uncover genetic and epigenetic fluctuation such as abnormal DNA methylation in gene promoter regions that is expected to play a key role in GC. The study of gastric malignancies requires an etiological perspective, and Helicobacter pylori (H. pylori) was identified to play a role in GC. H. pylori infection causes chronic inflammation of the gastric epithelium causing abnormal polyclonal methylation, which might raise the risk of GC. In the last two decades, various pathogenic factors by which H. pylori infection causes GC have been discovered. Abnormal DNA methylation is triggered in several genes, rendering them inactive. In GC, methylation patterns are linked to certain subtypes including microsatellite instability. Multiple cancer-related processes are more usually changed by abnormal DNA methylation than through mutations, according to current general and combined investigations. Furthermore, the amount of acquired abnormal DNA methylation is heavily linked to the chances of developing GC. Therefore, we investigated abnormal DNA methylation in GC and the link between methylation and H. pylori infection.

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DNA Methylation Profiling of hTERT Gene Alongside with the Telomere Performance in Gastric Adenocarcinoma

Cited by 9 publications

References 90 publications

Immortalization of human dermal microvascular endothelial cells

Immortalization of human dermal microvascular endothelial cells

Molecular Mechanisms of lncRNAs in the Dependent Regulation of Cancer and Their Potential Therapeutic Use

Performance of DNA Methylation on the Molecular Pathogenesis of Helicobacter pylori in Gastric Cancer; targeted therapy approach

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