DNA methylation of ghrelin and leptin receptors in underweight and recovered patients with anorexia nervosa

Batury, Victoria-Luise; Walton, Esther; Tam, Friederike I.; Wronski, Marie‐Louis; Buchholz, Vanessa; Frieling, Helge; Ehrlich, Stefan

doi:10.1016/j.jpsychires.2020.08.026

Cited by 12 publications

(5 citation statements)

References 52 publications

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“…Therefore, it may be assumed that in oncologic patients ghrelin supplementation could alleviate the symptoms of cancer cachexia [74]. Moreover, Batury et al showed that methylation of the DNA promoter region of ghrelin is a characteristic of patients devastated by anorexia [75]. Wolf et al also showed an increase in the level of ghrelin methylation in patients 6 months after bariatric surgery in comparison with the period prior to the operation [76], which confirms our assumptions and results.…”

Section: Discussionsupporting

confidence: 89%

Impact of Nutritional Status of Patients with Head and Neck Squamous Cell Carcinoma on the Expression Profile of Ghrelin, Irisin, and Titin

Andruszko,

Szydłowski,

Grabarek

et al. 2024

Cancers

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The goal of this paper was the evaluation of the changes in the expression profile of irisin, ghrelin, and titin in the carcinoma tissue and in the blood of patients with head and neck squamous cell carcinoma (HNSCC), including determining the profile of their expression in relation to patient nutrition. The study included 56 patients with diagnosed squamous cell carcinoma of HNSCC in the T3 and T4 stages of the disease. Healthy control tissue specimens were collected from an area 10 mm outside the histologically negative margin. In turn, the blood and serum from the control group came from healthy volunteers treated for non-oncologic reasons (n = 70). The molecular analysis allowed us to determine the profile of irisin, ghrelin, and titin methylation, evaluate their expression on the level of mRNA (quantitative Reverse Transcription Polymerase Chain Reaction; qRT-PCR) and protein (Enzyme-Linked Immunosorbent Assay Reaction; ELISA) in the carcinoma tissue and the margin of healthy tissue, as well as in serum of patients in the study and control groups. At the start of our observations, a Body Mass Index (BMI) < 18.5 was noted in 42 of the patients, while six months after the treatment a BMI < 18.5 was noted in 29 patients. We also noted a decrease in the expression of irisin, ghrelin, and titin both on the level of mRNA and protein, as well as a potential regulation of their expression via DNA methylation. There is no convincing evidence that the proteins assayed in the present work are specific with regard to HNSSC.

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Section: Discussionsupporting

confidence: 89%

Impact of Nutritional Status of Patients with Head and Neck Squamous Cell Carcinoma on the Expression Profile of Ghrelin, Irisin, and Titin

Andruszko,

Szydłowski,

Grabarek

et al. 2024

Cancers

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“…Besides, lower DNA methylation of t h e L E P g e n e w a s a s s o c i a t e d w i t h a s i g n i fi c a n t hypermethylation during psychotherapeutic treatment and full recovery in AN patients (51), thus suggesting a predictive value of LEP methylation in identifying patients with a higher probability of recovery after treatment (51). Also hypomethylation of the GHS-R1A gene, that encodes for the ghrelin receptor, has been reported in AN patients, supporting the effect of environment on satiety and ghrelin resistance (52), and a possible effect of this epigenetic alterations in FHA patients (Figure 2).…”

Section: The Epigenetic Contribution To Fhamentioning

confidence: 82%

Epigenetics of functional hypothalamic amenorrhea

et al. 2022

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Functional hypothalamic amenorrhea (FHA) is a temporary infertility characterized by the suppression of the hypothalamic–pituitary–gonadal (HPG) axis, induced by the inhibition of the hypothalamic pulsatile secretion of the gonadotropin-releasing hormone (GnRH), in the presence of stressors, including eating disorders, excessive exercise, and psychological distress. Although the stressful factors that may lead to FHA are well-established, little is known about the inter-individual variability in response to stress and the consequent inhibition of the HPG axis. Not all women, indeed, manifest FHA in presence of stressful conditions. Recent studies highlighted a genetic contribution to FHA. Rare or polymorphic variants in genes that control the development and/or function of GnRH neurons may contribute, indeed, to the adaptability of the reproductive axis to stress factors. Also epigenetic changes have been associated with different pathways involved in the HPG axis and therefore, take part in FHA and confer a personal predisposition to anovulation consequent to a stressful event, or represent biological markers of response to stress. This review summarizes recent advances in the identification of the contribution of (epi)genetics to FHA and to long-term complications of functional amenorrhea, and reports insights into the involvement of additional genetic loci in FHA development on the bases of the clinical and molecular overlap with other gynecological and/or psychological conditions. Finally, we describe the promising application of induced pluripotent stem cells (iPSCs) as a new approach to investigate the molecular pathways involved in FHA.

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“… [ 126 ] Baeza-Velasco et al, 2021 AN-R, AN-BP, BN “Other ED” All ED types 31.03 (11.02) without JHM 22.77 (2.07) with JHM All ED types 19.31 (6.2) without JHM 20.22 (4.8) with JHM All ED types 12.93 (9.07) without JHM 6.25 (6.73) with JHM NA JHM MINI 5.0.0 EDI-2 ED without JHM: 13% male ED with JHM: no males 6. [ 127 ] Batury et al, 2020 AN-R, AN-BP 18.0 ± 3.4 (acute) 19.7 ± 3.8 (recovered) 15.0 ± 0.3 (acute) Not provided. Age of onset average provided.…”

Section: Methodsmentioning

confidence: 99%

Inclusion of the severe and enduring anorexia nervosa phenotype in genetics research: a scoping review

Ramsay,

Allison,

Temples

et al. 2024

J Eat Disord

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Background Anorexia nervosa has one of the highest mortality rates of all mental illnesses. For those who survive, less than 70% fully recover, with many going on to develop a more severe and enduring phenotype. Research now suggests that genetics plays a role in the development and persistence of anorexia nervosa. Inclusion of participants with more severe and enduring illness in genetics studies of anorexia nervosa is critical. Objective The primary goal of this review was to assess the inclusion of participants meeting the criteria for the severe enduring anorexia nervosa phenotype in genetics research by (1) identifying the most widely used defining criteria for severe enduring anorexia nervosa and (2) performing a review of the genetics literature to assess the inclusion of participants meeting the identified criteria. Methods Searches of the genetics literature from 2012 to 2023 were performed in the PubMed, PsycINFO, and Web of Science databases. Publications were selected per the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). The criteria used to define the severe and enduring anorexia nervosa phenotype were derived by how often they were used in the literature since 2017. The publications identified through the literature search were then assessed for inclusion of participants meeting these criteria. Results most prevalent criteria used to define severe enduring anorexia nervosa in the literature were an illness duration of ≥ 7 years, lack of positive response to at least two previous evidence-based treatments, a body mass index meeting the Diagnostic and Statistical Manual of Mental Disorders-5 for extreme anorexia nervosa, and an assessment of psychological and/or behavioral severity indicating a significant impact on quality of life. There was a lack of consistent identification and inclusion of those meeting the criteria for severe enduring anorexia nervosa in the genetics literature. Discussion This lack of consistent identification and inclusion of patients with severe enduring anorexia nervosa in genetics research has the potential to hamper the isolation of risk loci and the development of new, more effective treatment options for patients with anorexia nervosa.

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DNA methylation of ghrelin and leptin receptors in underweight and recovered patients with anorexia nervosa

Cited by 12 publications

References 52 publications

Impact of Nutritional Status of Patients with Head and Neck Squamous Cell Carcinoma on the Expression Profile of Ghrelin, Irisin, and Titin

Impact of Nutritional Status of Patients with Head and Neck Squamous Cell Carcinoma on the Expression Profile of Ghrelin, Irisin, and Titin

Epigenetics of functional hypothalamic amenorrhea

Inclusion of the severe and enduring anorexia nervosa phenotype in genetics research: a scoping review

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