DNA methylation control of tissue polarity and cellular differentiation in the mammary epithelium

Plachot, Cédric; Lelièvre, Sophie A.

doi:10.1016/j.yexcr.2004.04.024

Cited by 79 publications

(99 citation statements)

References 51 publications

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“…In this work, we demonstrate that NuMA is abundant also in the chromatin fraction. The presence of NuMA in different compartments of the cell nucleus may explain the great variation in its distribution reported for different cell and tissue phenotypes (Sodja et al, 1997;Merdes and Cleveland, 1998;Lelièvre et al, 1998;Gribbon et al, 2002;Plachot and Lelièvre, 2004;Knowles et al, 2006). The compartmentalization of NuMA is particularly interesting to observe upon DNase I treatment in situ.…”

Section: Discussionmentioning

confidence: 99%

“…Nonneoplastic S1 HMT-3522 HMECs (Briand et al, 1987), between passages 52 and 60, were plated at 2.3 ϫ 10 4 cells/cm 2 for propagation as monolayers (two-dimentsional [2D] culture) on plastic (Falcon; BD Biosciences Discovery Labware, Bedford, MA) in chemically defined H14 medium (Blaschke et al, 1994;Plachot and Lelièvre, 2004). To induce acinar differentiation in 3D culture, S1 cells were plated in the presence of 5% drip laminin-rich extracellular matrix (Matrigel; BD Biosciences Discovery Labware), as described previously, and kept for 10 d in H14 medium ; the 3D cell culture procedure is reviewed in Lelièvre and Bissell, 2005).…”

Section: Cell Culture and Induction Of Acinar Differentiationmentioning

confidence: 99%

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NuMA Influences Higher Order Chromatin Organization in Human Mammary Epithelium

Abad

Lewis

Mian

et al. 2007

MBoC

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The coiled-coil protein NuMA is an important contributor to mitotic spindle formation and stabilization. A potential role for NuMA in nuclear organization or gene regulation is suggested by the observations that its pattern of nuclear distribution depends upon cell phenotype and that it interacts and/or colocalizes with transcription factors. To date, the precise contribution of NuMA to nuclear function remains unclear. Previously, we observed that antibody-induced alteration of NuMA distribution in growth-arrested and differentiated mammary epithelial structures (acini) in threedimensional culture triggers the loss of acinar differentiation. Here, we show that in mammary epithelial cells, NuMA is present in both the nuclear matrix and chromatin compartments. Expression of a portion of the C terminus of NuMA that shares sequence similarity with the chromatin regulator HPC2 is sufficient to inhibit acinar differentiation and results in the redistribution of NuMA, chromatin markers acetyl-H4 and H4K20m, and regions of deoxyribonuclease I-sensitive chromatin compared with control cells. Short-term alteration of NuMA distribution with anti-NuMA C-terminus antibodies in live acinar cells indicates that changes in NuMA and chromatin organization precede loss of acinar differentiation. These findings suggest that NuMA has a role in mammary epithelial differentiation by influencing the organization of chromatin. INTRODUCTIONThe nuclear mitotic apparatus protein (NuMA) was first described in 1980 (Lydersen and Pettijohn, 1980) and observed to concentrate at the spindle poles during mitosis. Subsequently NuMA, variously named SPN antigen, p240 antigen, centrophilin, 1F1/1H1 antigen, SP-H antigen, and W1 antigen (Compton et al., 1991(Compton et al., , 1992Kallajoki et al., 1991Kallajoki et al., , 1993Maekawa et al., 1991;Tousson et al., 1991;Yang et al., 1992;Maekawa and Kuriyama, 1993;Tang et al., 1993), was shown to be a critical player in the formation and stabilization of the mitotic spindle, notably by associating with the minus end of spindle microtubules and interacting with dynein, dynactin, and LGN (Compton and Cleveland, 1993;Gaglio et al., 1995;Merdes et al., 2000;Du et al., 2001;Gehmlich et al., 2004). In addition to its location at the poles of the mitotic spindle, NuMA has been reported in the nucleus of both cycling and growth-arrested cells, as shown by immunostaining of cells in culture and tissue biopsy sections (Lelièvre et al., 1998;Merdes and Cleveland 1998;Gribbon et al., 2002;Taimen et al., 2004). However, a role for NuMA in interphase remains to be determined. The hypothesis that NuMA might organize nuclear structure (Compton and Cleveland, 1994) is supported by the existence of a long central coiled-coil region in the protein and the prevalence of NuMA in the nucleus upon detergent extraction. Furthermore, NuMA binds DNA matrix attachment regions (MARs) in vitro (Ludérus et al., 1994), and the cleavage of NuMA precedes DNA degradation during apoptosis (Weaver et al., 1996). The likelihood of a link...

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Section: Discussionmentioning

confidence: 99%

Section: Cell Culture and Induction Of Acinar Differentiationmentioning

confidence: 99%

NuMA Influences Higher Order Chromatin Organization in Human Mammary Epithelium

Abad

Lewis

Mian

et al. 2007

MBoC

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“…68 Blocking DNA methylation during the differentiation of mammary epithelial cells in cell culture models resulted in incomplete differentiation and increased expression of the putative mammary epithelial stem cell marker cytokeratin 19. 69 These findings imply that alterations in DNA methylation patterns might play a role in mammary gland development.…”

Section: Epigenetic Programs Of Adult Stem Cellsmentioning

confidence: 96%

Epigenetic patterns of embryonic and adult stem cells

Bloushtain-Qimron

Yao²,

Shipitsin

et al. 2009

Cell Cycle

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“…66,67 In this regard, Plachot 66 suggested that DNA methylation is a mechanism by which mammary epithelial differentiation is coordinated at both cellular and tissue levels; he found acinar morphogenesis to be accompanied by chromatin remodeling and increased expression of MeCP-2, a mediator of DNA-methylation-induced gene silencing, revealing that the epigenome can be influenced by the microenvironment.…”

Section: Epigenetic Control Of Tumor Developmentmentioning

confidence: 99%