DNA Methylation-Based Method to Differentiate Malignant from Benign Thyroid Lesions

Barros-Filho, Mateus Camargo; Reis, Mariana Bisarro dos; Beltrami, Caroline Moraes; Mello, Julia Bette Homem de; Marchi, Fábio Albuquerque; Kuasne, Hellen; Drigo, Sandra A.; Andrade, Victor Piana de; Saieg, Mauro; Pinto, Clóvis Antônio Lopes; Kowalski, Luiz Paulo; Rogatto, Sílvia Regina

doi:10.1089/thy.2018.0458

Cited by 17 publications

(9 citation statements)

References 59 publications

(67 reference statements)

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“…Nucleic acids (DNA and RNA) were isolated, as previously described (26). Adequate quantity and quality for 202 DNA and 178 RNA PTC specimens were obtained.…”

Section: Detection Of Genomic Alterationsmentioning

confidence: 99%

GADD45B Transcript Is a Prognostic Marker in Papillary Thyroid Carcinoma Patients Treated With Total Thyroidectomy and Radioiodine Therapy

et al. 2020

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Currently, there is a lack of efficient recurrence prediction methods for papillary thyroid carcinoma (PTC). In this study, we enrolled 202 PTC patients submitted to total thyroidectomy and radioiodine therapy with long-term follow-up (median = 10.7 years). The patients were classified as having favorable clinical outcome (PTC-FCO, no disease in the follow-up) or recurrence (PTC-RE). Alterations in BRAF, RAS, RET, and TERT were investigated (n = 202) and the transcriptome of 48 PTC (>10 years of follow-up) samples was profiled. Although no mutation was associated with the recurrence risk, 68 genes were found as differentially expressed in PTC-RE compared to PTC-FCO. Pathway analysis highlighted a potential role of cancer-related pathways, including signal transduction and FoxO signaling. Among the eight selected genes evaluated by RT-qPCR, SLC2A4 and GADD45B showed down-expression exclusively in the PTC-FCO group compared to non-neoplastic tissues (NT). Increased expression of GADD45B was an independent marker of shorter disease-free survival [hazard ratio (HR) 2.9; 95% confidence interval (CI95) 1.2-7.0] in our cohort and with overall survival in the TCGA dataset (HR = 4.38, CI95 1.2-15.5). In conclusion, GADD45B transcript was identified as a novel prognostic marker candidate in PTC patients treated with total thyroidectomy and radioiodine therapy.

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“…Nucleic acids (DNA and RNA) were isolated, as previously described (26). Adequate quantity and quality for 202 DNA and 178 RNA PTC specimens were obtained.…”

Section: Detection Of Genomic Alterationsmentioning

confidence: 99%

GADD45B Transcript Is a Prognostic Marker in Papillary Thyroid Carcinoma Patients Treated With Total Thyroidectomy and Radioiodine Therapy

et al. 2020

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“…DNA methylation analysis revealed 2130 and 19 differentially methylated CpGs in PTC and follicular thyroid carcinoma (FTC), respectively [46]. Aberrant DNA methylation of tumor suppressor genes is common in FTC and ATC.…”

Section: Dna Methylation In Ftcmentioning

confidence: 99%

Research Progress of DNA Methylation in Thyroid Cancer

Zhu¹,

Xie²

2020

DNA Methylation Mechanism

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We have summarized increasing data from all kinds of experiment results of papers in recent years, which are associated with tumor suppressor genes, oncogenes, and thyroid-specific genes and attempt to elucidate the importance of epigenetic modifications and the mechanisms of aberrant DNA methylation in thyroid cancer in this review. The results showed that current articles have revealed the importance of epigenetic modifications and the different types of mechanisms in thyroid cancer. The mechanisms of DNA methylation related to thyroid cancer demonstrate that acquired epigenetic abnormalities together with genetic changes play an important role in alteration of gene expression patterns. Aberrant DNA methylation has been well known in the CpG regions. Among the genes identified, we have shown the status of DNA promoter methylation in papillary, follicular, medullary, and anaplastic thyroid cancer. It suggested that thyroid cancer subtypes present differential promoter methylation signatures, which will encourage potential thyroid cancer detection in its early stages, assessment of prognosis, and targeted cancer treatment.

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“…Since the detection of DNA methylation signatures of thyroid nodules is considered to be a reliable method to differentiate malignant and benign thyroid lesions [49][50][51][52][53], methylation can explain a synergic detrimental effect of obesity and CMV replication on the thyroid. Metabolic, endocrine or cytokine secretions may also be involved in the inflammatory process.…”

Section: Sci Forschenmentioning

confidence: 99%

Ongoing CMV Infection (qPCR+), Nodular Thyroiditis and Periodontitis are Associated with Ileal Distension (Ileal Brake), Cancer and Increased Plasmatic Hyaluronic Acid Levels

Donatini¹,

Blaye²

2020

J Clin Case Stu

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Some cancers have been attributed to Fusobacterium nucleatum (which belongs to PO-associated pathogens), especially colonic cancer [14].The severity of PO is also associated (even attributed by some authors) to the expression of herpes viruses, including cytomegalovirus [15][16][17][18]. The association of cytomegalovirus and Aggregatibacter actinomycetemcomitans could explain severe progression of PO [19].Multiple herpesvirus infection is detected in patients with severe chronic PO. Herpes Simplex Virus (HSV)-1 (46.6%) are the most common herpesvirus followed by HSV-2 (34.6%), Epstein-Barr Viruses (EBV) (30.6%), and Cytomegalovirus (CMV) (19.3%) [20]. Cytomegalovirus has also been implicated in the occurrence of central

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DNA Methylation-Based Method to Differentiate Malignant from Benign Thyroid Lesions

Cited by 17 publications

References 59 publications

GADD45B Transcript Is a Prognostic Marker in Papillary Thyroid Carcinoma Patients Treated With Total Thyroidectomy and Radioiodine Therapy

GADD45B Transcript Is a Prognostic Marker in Papillary Thyroid Carcinoma Patients Treated With Total Thyroidectomy and Radioiodine Therapy

Research Progress of DNA Methylation in Thyroid Cancer

Ongoing CMV Infection (qPCR+), Nodular Thyroiditis and Periodontitis are Associated with Ileal Distension (Ileal Brake), Cancer and Increased Plasmatic Hyaluronic Acid Levels

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