DNA methylation and smoking in Korean adults: epigenome-wide association study

Lee, Mi Kyeong; Hong, Yoonki; Kim, Sun‐Young; London, Stephanie J.; Kim, Woo Jin

doi:10.1186/s13148-016-0266-6

Cited by 49 publications

(46 citation statements)

References 52 publications

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“…DNA methylation patterns can be modified by various environmental and lifestyle factors including cigarette smoking (Inbar‐Feigenberg, Choufani, Butcher, Roifman, & Weksberg, ; Lee & Pausova, ). Over the recent years, the number of studies in the literature investigating the relationship between DNA methylation and smoking has been on the rise (Al Khaled, Tierling, Laqqan, Lo Porto, & Hammadeh, ; Ambatipudi et al, ; Guida et al, ; Klebaner et al, ; Kobayashi et al, ; Lee, Hong, Kim, London, & Kim, ; Zeilinger et al, ; Zhu et al, ). Two mechanisms regarding smoking‐induced DNA have been proposed to explain methylation alterations.…”

Section: Cigarette Smoking and Epigenetic Alterationsmentioning

confidence: 99%

Smoking-induced genetic and epigenetic alterations in infertile men

et al. 2018

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Male fertility rates have shown a progressive decrease in both developing and industrialised countries in the past 50 years. Clinical and epidemiological studies have demonstrated controversial results about the harmful effects of cigarette smoking on seminal parameters. Some studies could not establish a negative effect by tobacco smoking on sperm quality and function, whereas others have found a significant reduction in sperm quality and function. This study reviews the components in cigarette smoke and discusses the effects of smoking on male fertility by focusing extensively on smoking-induced genetic and epigenetic alterations in infertile men. Chromosomal aneuploidies, sperm DNA fragmentation and gene mutations are discussed in the first section, while changes in DNA methylation, chromatin remodelling and noncoding RNAs are discussed in the second section as part of epigenetic alterations.

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Section: Cigarette Smoking and Epigenetic Alterationsmentioning

confidence: 99%

Smoking-induced genetic and epigenetic alterations in infertile men

et al. 2018

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“…In addition, as tobacco smoke is known to affect DNA methylation profile of blood cells [Belinsky et al, ; Breitling et al, ; Shenker et al, ; Harlid et al, ; Allione et al, ; Lee et al, ] and TA may be modulated by epigenetic changes affecting its catalytic subunit ( hTERT ) [Lewis and Tollefsbol, ], the methylation status of hTERT gene was investigated to assess the possible association between smoke‐induced epigenetic modifications of hTERT and TA.…”

Section: Introductionmentioning

confidence: 99%

Telomerase activity, telomere length and hTERT DNA methylation in peripheral blood mononuclear cells from monozygotic twins with discordant smoking habits

Marcon

Siniscalchi

Andreoli

et al. 2017

Environ and Mol Mutagen

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Increased telomerase expression has been implicated in the pathogenesis of lung cancer and, since the primary cause of lung cancer is smoking, an association between telomerase reactivation and tobacco smoke has been proposed. In this work an investigation has been performed to assess the relationship between tobacco smoke exposure and telomerase activity (TA) in peripheral blood mononuclear cells of healthy smokers. The methylation status of the catalytic subunit of telomerase hTERT was concurrently investigated to assess the possible association between epigenetic modifications of hTERT and TA. Besides, the association between smoke and telomere length (TL) has been evaluated. Healthy monozygotic twins with discordant smoking habits were selected as study population to minimize inter-individual differences because of demographic characteristics and genetic heterogeneity. Statistically significant higher values of TA and TL were observed in smokers compared to nonsmoker co-twins. The multivariate analysis of data showed, besides smoking habits (P = 0.02), an influence of gender (P = 0.006) and BMI (P = 0.001) on TA and a borderline effect of gender (P = 0.05) on TL. DNA methylation analysis, focused on 100 CpG sites mapping in hTERT, highlighted nine CpG sites differentially methylated in smokers. When co-twins were contrasted, selecting as variables the intra-twin difference in TA and hTERT DNA methylation, a statistically significant inverse correlation (P = 0.003) was observed between TA and DNA methylation at the cg05521538 site. In conclusion, these results indicate an association of tobacco smoke with TA and TL and suggest a possible association between smoke-induced epigenetic effects and TA in healthy smokers. Environ. Mol. Mutagen. 58:551-559, 2017. © 2017 Wiley Periodicals, Inc.

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“…None of the 22 CpG sites that we identified were reported in previous EWAS of cigarette smoking in African ancestry [24,25] or Asian ancestry [5,26,27], and only one (cg03026462 at FOXA1) was identified in the meta-analysis of European and African ancestry [4]. Thus, we identified 21 novel loci for cigarette smoking that have not been detected in previous epigenetic research on smoking.…”

Section: Discussionmentioning

confidence: 58%

Longitudinal analysis of epigenome-wide DNA methylation reveals novel smoking-related loci in African Americans

et al. 2019

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Changes in DNA methylation may be a potential mechanism that mediates the effects of smoking on physiological function and subsequent disease risk. Given the dynamic nature of the epigenome, longitudinal studies are indispensable for investigating smoking-induced methylation changes over time. Using blood samples collected approximately five years apart in 380 African Americans (mean age 60.7 years) from the Genetic Epidemiology Network of Arteriopathy (GENOA) study, we measured DNA methylation levels using Illumina HumanMethylation BeadChips. We evaluated the association between Phase 1 smoking status and rate of methylation change, using generalized estimating equation models. Among the 6958 CpG sites examined, smoking status was associated with methylation change for 22 CpGs (false discovery rate q < 0.1), with the majority (91%) becoming less methylated over time. Methylation change was greater in ever smokers than never smokers, and the absolute differences in rates of change ranged from 0.18 to 0.77 per decade in M value, equivalent to a β value change of 0.013 to 0.047 per decade. Significant enrichment was observed for CpG islands, enhancers, and DNAse hypersensitivity sites (p < 0.05). Although biological pathway analyses were not significant, most of the 22 CpGs were within genes known to be associated with cardiovascular disease, cancers, and aging. In conclusion, we identified epigenetic signatures for cigarette smoking that may have been missed in cross-sectional analyses, providing insight into the epigenetic effect of smoking and highlighting the importance of longitudinal analysis in understanding the dynamic human epigenome.

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DNA methylation and smoking in Korean adults: epigenome-wide association study

Cited by 49 publications

References 52 publications

Smoking-induced genetic and epigenetic alterations in infertile men

Smoking-induced genetic and epigenetic alterations in infertile men

Telomerase activity, telomere length and hTERT DNA methylation in peripheral blood mononuclear cells from monozygotic twins with discordant smoking habits

Longitudinal analysis of epigenome-wide DNA methylation reveals novel smoking-related loci in African Americans

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