DNA methylation and adiposity phenotypes: an epigenome-wide association study among adults in the Strong Heart Study

Crocker, Katherine C.; Domingo‐Relloso, Arce; Haack, Karin; Fretts, Amanda M.; Tang, Wan Yee; Herreros, Miguel; Téllez-Plaza, María; Fallin, M. Daniele; Cole, Shelley A.; Navas‐Acien, Ana

doi:10.1038/s41366-020-0646-z

Cited by 16 publications

(12 citation statements)

References 47 publications

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“…Several speci c genes regulate these steps and the expression of these genes in turn is regulated by DNA methylation, histone modi cations and speci c miRNAs 42,43,44 . The role of DNA methylation in adipogenesis has been studied 45,46 but there is little information on the other epigenetic mechanisms (i.e., miRNA regulation). Light microscopic studies in human abortuses demonstrate that pre-adipocyte to adipocyte differentiation happens between 19 to 23 weeks, after which period only adipocyte size increases by lipid accumulation 7 .…”

Section: Discussionmentioning

confidence: 99%

Neonatal Adiposity Is Associated with microRNAs in Adipocyte-Derived Extracellular Vesicles in Maternal and Cord Blood

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Neonatal Adiposity Is Associated with microRNAs in Adipocyte-Derived Extracellular Vesicles in Maternal and Cord Blood

et al. 2022

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“…Several specific genes regulate these steps and the expression of these genes in turn is regulated by DNA methylation, histone modifications and specific miRNAs 42,43,44 . The role of DNA methylation in adipogenesis has been studied 45,46 but there is little information on the other epigenetic mechanisms (i.e., miRNA regulation). Light microscopic studies in human abortuses demonstrate that pre-adipocyte to adipocyte differentiation happens between 19 to 23 weeks, after which period only adipocyte size increases by lipid accumulation 7 .…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs in adipocyte-derived extracellular vesicles in maternal and cord blood are related to neonatal adiposity

Kunte¹,

Barberio

Tiwari³

et al. 2022

Preprint

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Background: Maternal body size, nutrition, and hyperglycemia contribute to neonatal body size and composition. There is little information on maternal-fetal transmission of messages which influence fetal growth. We analyzed adipocyte-derived small extracellular vesicular (ADsEV) microRNAs in maternal and cord blood to explore their adipogenic potential. Methods: We studied 127 mother-neonate pairs (51 lean and 76 adipose neonates, in 68 NGT and 59 GDM pregnancies). Adiposity refers to highest tertile (T3) of sum of skinfolds in neonates of normal glucose tolerant (NGT) mothers, lean to lowest tertile (T1). ADsEV miRNAs from maternal and cord blood samples were profiled on Agilent 8*60K microarray. Differential expression (DE) of ADsEV miRNAs in adipose vs. lean neonates was studied before and after adjustment for maternal gestational diabetes mellitus (GDM), adiposity, and vitamin B12-folate status. Results: Multiple miRNAs were common in maternal and cord blood and positively correlated. We identified 24 maternal and 5 cord blood miRNAs differentially expressed (p≤0.1) in the adipose neonate group, and 19 and 26 respectively, in the adjusted analyses. Even though DE miRNAs were different in maternal and cord blood, they targeted similar adipogenic pathways (e.g., the forkhead box O (FOXO) family of transcription factors, mitogen‑activated protein kinase (MAPK) pathway, transforming growth factor beta (TGF-β) pathway). Maternal GDM and adiposity were associated with many DE ADsEV miRNAs. Conclusion: Our results suggest that the DE ADsEV miRNAs in mothers of adipose neonates are potential regulators of fetal adiposity. Further, the composition and functionality of miRNAs may be influenced by maternal hyperglycemia, adiposity, and micronutrient status during pregnancy .

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“…A previous study [ 50 ] of lean, young Norwegian women and Norwegian women with obesity ( N = 120, mean age = 27.2), identified 10 CpG sites across eight genes associated with obesity (Supporting Information Table S5 ) [ 1 , 4 , 6 , 8 , 9 , 10 , 15 , 16 , 17 , 18 , 43 , 47 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 ]. We identified significantly different methylation at CpG sites in three of the same genes in our discovery cohort ( SOCS3, SBNO2 , and FGD2 ) and three CpG sites located in the gene bodies of SOCS3 (cg18181703) and SBNO2 (cg12170787, cg18608055).…”

Section: Discussionmentioning

confidence: 99%

Epigenome‐wide association study of BMI in Black populations from InterGEN and GENOA

Taylor

Huang

Zhao

et al. 2022

Obesity

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Objective Obesity is a significant public health concern across the globe. Research investigating epigenetic mechanisms related to obesity and obesity‐associated conditions has identified differences that may contribute to cellular dysregulation that accelerates the development of disease. However, few studies include Black women, who experience the highest incidence of obesity and early onset of cardiometabolic disorders. Methods The association of BMI with epigenome‐wide DNA methylation (DNAm) was examined using the 850K Illumina EPIC BeadChip in two Black populations (Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure [InterGEN], n = 239; and The Genetic Epidemiology Network of Arteriopathy [GENOA] study, n = 961) using linear mixed‐effects regression models adjusted for batch effects, cell type heterogeneity, population stratification, and confounding factors. Results Cross‐sectional analysis of the InterGEN discovery cohort identified 28 DNAm sites significantly associated with BMI, 24 of which had not been previously reported. Of these, 17 were replicated using the GENOA study. In addition, a meta‐analysis, including both the InterGEN and GENOA cohorts, identified 658 DNAm sites associated with BMI with false discovery rate < 0.05. In a meta‐analysis of Black women, we identified 628 DNAm sites significantly associated with BMI. Using a more stringent significance threshold of Bonferroni‐corrected p value 0.05, 65 and 61 DNAm sites associated with BMI were identified from the combined sex and female‐only meta‐analyses, respectively. Conclusions This study suggests that BMI is associated with differences in DNAm among women that can be identified with DNA extracted from salivary (discovery) and peripheral blood (replication) samples among Black populations across two cohorts.

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DNA methylation and adiposity phenotypes: an epigenome-wide association study among adults in the Strong Heart Study

Cited by 16 publications

References 47 publications

Neonatal Adiposity Is Associated with microRNAs in Adipocyte-Derived Extracellular Vesicles in Maternal and Cord Blood

Neonatal Adiposity Is Associated with microRNAs in Adipocyte-Derived Extracellular Vesicles in Maternal and Cord Blood

MicroRNAs in adipocyte-derived extracellular vesicles in maternal and cord blood are related to neonatal adiposity

Epigenome‐wide association study of BMI in Black populations from InterGEN and GENOA

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