DNA Methylation Analysis

Feng, Lingfang; Lou, Jianlin

doi:10.1007/978-1-4939-8916-4_12

Cited by 44 publications

(30 citation statements)

References 30 publications

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“…β-actin and U6 were used as internal references for measuring relative expression of DNMT1 and miR-29b-3p, respectively. The expressions of genes were quantified using the 2 -∆∆Cq method (22).…”

Section: Transient Transfection For F Unctional Analysis Of Mir-29b-3mentioning

confidence: 99%

Methylation of the miR‑29b‑3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer

Wang

2020

Oncol Rep

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The aim of the present study was to investigate the effects of microRNA (miR)-29b-3p gene promoter methylation on angiogenesis, invasion, and migration in human pancreatic cancer. Prediction of promoter methylation of miR-29b-3p was performed through the MethPrimer tool. Then the methylation levels of miR-29b-3p in human pancreatic cancer tissues and cell lines were detected by pyrosequencing, and the relative expression of miR-29b-3p was assessed in pancreatic cancer tissues by qPCR. The results were analyzed by linear regression. Western blot analysis was used to detect expression of DNA methyltransferases (DNMTs) in pancreatic cancer tissues and adjacent tissues. The Transwell assay was used to detect the ability of cell migration and invasion. Cells were co-cultured with human umbilical vein endothelial cells (HUVECs) to detect the ability of angiogenesis. The results revealed that DNMT1 expression in pancreatic cancer tissues was higher than that in adjacent tissues. Further results showed that expression of miR-29b was negatively correlated with the methylation level of the miR-29b promoter. Bxpc3 and Capan-2 cells had higher methylation levels, and the expression level of miR-29b-3p in Bxpc3 and Capan-2 cells was found to be lower than that of other cell lines. Expression of zonula occludens-1 (ZO-1) and occludin was significantly increased, and the migration of cancer cells was decreased after cells were treated with siRNA DNMT1. Further results showed that miR-29b reversed the promotive effect of DNMT1 overexpression on tumor cell malignant properties. Methylation of the miR-29b-3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer. This study indicated that the alteration of methylation of mR-19b may be a potential approach for inhibiting the progression of pancreatic cancer.

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Section: Transient Transfection For F Unctional Analysis Of Mir-29b-3mentioning

confidence: 99%

Methylation of the miR‑29b‑3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer

Wang

2020

Oncol Rep

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“…More specifically, matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis, where the sample is ionized by transferring energy from a surrounding matrix after a laser pulse and these ions are then accelerated in a mass spectrometer to measure time of flight-is used to detect epigenetic modifications [20,21]. For example in renal cell carcinoma, it has been shown that MALDI-TOF can be used to reproducibly quantify the methylation status of individual DNA bases [2,19,22,23]. In addition, bisulfite-converted DNA can be subjected to high resolution melt analysis.…”

Section: Detection Of Dna Methylationmentioning

confidence: 99%

The Zebrafish Model to Understand Epigenetics in Renal Diseases

Sopel

Müller-Deile

2021

IJMS

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Epigenetic modifications are able to alter gene expression and include DNA methylation, different histone variants, and post-transcriptional modifications (PTMs), such as acetylation or phosphorylation, and through short/long RNAs, respectively. In this review, we focus on current knowledge concerning epigenetic modifications in gene regulation. We describe different forms of epigenetic modifications and explain how epigenetic changes can be detected. The relevance of epigenetics in renal diseases is highlighted with multiple examples and the use of the zebrafish model to study glomerular diseases in general and epigenetics in renal diseases in particular is discussed. We end with an outlook on how to use epigenetic modifications as a therapeutic target for different diseases. Here, the zebrafish model can be employed as a high-throughput screening tool not only to discover epigenetic alterations contributing to disease, but also to test novel substances that change epigenetic signatures in vivo. Therefore, the zebrafish model harbors the opportunity to find novel pathogenic pathways allowing a pre-selection of potential targets and compounds to be tested for renal diseases.

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“…However, the biological significance of 5hmC in human cancer remains elusive [54]. Discovery of the mechanism for ten-eleven translocation enzymes (TET1, TET2, and TET3), which are capable of the oxidation 5mC to 5hmC and gene regulation, shows that cytosine methylation is essential in mammalian genomic DNA and transcriptional regulation [55,56]. Global loss of 5hmC, associated with TET downregulation and alteration of TET functions, points to a link between cancer epigenetics and immunoregulation [57,58].…”

Section: The Potential Value Of 5hmc In Different Types Of Cancermentioning

confidence: 99%

Hydroxymethylation and tumors: can 5-hydroxymethylation be used as a marker for tumor diagnosis and treatment?

Gao

2020

Hum Genomics

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5-Methylcytosine (5mC) is considered as a common epigenetic modification that plays an important role in the regulation of gene expression. At the same time, 5-hydroxymethylcytosine (5hmC) has been found as an emerging modification of cytosine bases of recent years. Unlike 5mC, global 5hmC levels vary from tissues that have differential distribution both in mammalian tissues and in the genome. DNA hydroxymethylation is the process that 5mC oxidates into 5hmC with the catalysis of TET (ten-eleven translocation) enzymes. It is an essential option of DNA demethylation, which modulates gene expression by adjusting the DNA methylation level. Various factors can regulate the demethylation of DNA, such as environmental toxins and mental stress. In this review, we summarize the progress in the formation of 5hmC, and obtaining 5hmC in a cell-free DNA sample presents multiple advantages and challenges for the subject. Furthermore, the clinical potential for 5hmC modification in dealing with cancer early diagnosis, prognostic evaluation, and prediction of therapeutic effect is also mentioned.

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DNA Methylation Analysis

Cited by 44 publications

References 30 publications

Methylation of the miR‑29b‑3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer

Methylation of the miR‑29b‑3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer

The Zebrafish Model to Understand Epigenetics in Renal Diseases

Hydroxymethylation and tumors: can 5-hydroxymethylation be used as a marker for tumor diagnosis and treatment?

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