DNA-Mediated Homogeneous Binding Assays for Nucleic Acids and Proteins

Zhang, Hongquan; Li, Feng; Dever, Brittany; Li, Xing‐Fang; Le, X. Chris

doi:10.1021/cr300340p

Cited by 387 publications

(242 citation statements)

References 315 publications

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“…Given the amount of knowledge accumulated in the past two decade, this is a good time to critically summarize this field and identify new solutions to move forward. Numerous review papers have already been published on various aspects of the aptamer biosensor field at different stages of development, 4,16,[18][19][20][21][22][23][24][25][26][27][28] and we do not intend to provide a comprehensive review here. In this Critical Review, we first briefly describe aptamer selection and general biosensor design strategies, keeping in mind biomedical diagnosis as the application goal.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-based biosensors for biomedical diagnostics

Zhou

Huang

Ding

et al. 2014

Analyst

449

282

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Aptamers are single-stranded nucleic acids that selectively bind to target molecules. Most aptamers are obtained through a combinatorial biology technique called SELEX. Since aptamers can be isolated to bind to almost any molecule of choice, can be readily modified at arbitrary positions and they possess predictable secondary structures, this platform technology shows great promise in biosensor development. Over the past two decades, more than one thousand papers have been published on aptamer-based biosensors. Given this progress, the application of aptamer technology in biomedical diagnosis is still in a quite preliminary stage. Most previous work involves only a few model aptamers to demonstrate the sensing concept with limited biomedical impact. This Critical Review aims to summarize progresses that might enable practical applications of aptamer for biological samples. First, general sensing strategies based on the unique properties of aptamers are summarized. Each strategy can be coupled to various signaling methods. Among these, a few detection methods including fluorescence lifetime, flow cytometry, upconverting nanoparticles, nanoflare technology, magnetic resonance imaging, electronic aptamer-based sensors, and lateral flow devices have been discussed in more detail since they are more likely to work in a complex sample matrix. The current limitations of this field include the lack of high quality aptamers for clinically important targets. In addition, the aptamer technology has to be extensively tested in a clinical sample matrix to establish reliability and accuracy. Future directions are also speculated to overcome these challenges.3

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Section: Introductionmentioning

confidence: 99%

Aptamer-based biosensors for biomedical diagnostics

Zhou

Huang

Ding

et al. 2014

Analyst

449

282

View full text Add to dashboard Cite

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“…Homogeneous methods are especially relevant for the development of in-vivo techniques, where direct investigation of processes in live cells is of key importance. 8 To that end, numerous types of nanoparticles have been developed.…”

Section: Introductionmentioning

confidence: 99%

Homogeneous Protein Analysis by Magnetic Core–Shell Nanorod Probes

Schrittwieser

Pelaz

Parak

et al. 2016

ACS Appl. Mater. Interfaces

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Studying protein interactions is of vital importance both to fundamental biology research and to medical applications. Here, we report on the experimental proof of a universally applicable labelfree homogeneous platform for rapid protein analysis. It is based on optically detecting changes in the rotational dynamics of magnetically agitated core-shell nanorods upon their specific interaction with proteins. By adjusting the excitation frequency, we are able to optimize the measurement signal for each analyte protein size. In addition, due to the locking of the optical signal to the magnetic excitation frequency, background signals are suppressed, thus allowing exclusive studies of processes at the nanoprobe surface only. We study target proteins (soluble domain of the human epidermal growth factor receptor 2 -sHER2) specifically binding to antibodies (trastuzumab) immobilized on the surface of our nanoprobes and demonstrate direct deduction of their respective sizes. Additionally, we examine the dependence of our measurement signal on the concentration of the analyte protein, and deduce a minimally detectable sHER2 concentration of 440 pM. For our homogeneous measurement platform, good dispersion stability of the applied nanoprobes under physiological conditions is of vital importance. To that end, we support our measurement data by theoretical modeling of the total particle-particle interaction energies. The successful implementation of our platform offers scope for applications in biomarker-based diagnostics as well as for answering basic biology questions.

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“…homogeneous assay), all the reagents are mixed in a vessel and reactions are intended to take place only in the solution phase. [5][6][7][8][9][10][11] Hydrophobic plastics such as polystyrene and polypropylene are the most common container materials for making disposable microplates and microtubes. The interaction between biomolecules and surfaces in homogeneous assays is often ignored.…”

Section: Introductionmentioning

confidence: 99%

Parts-per-Million of Polyethylene Glycol as a Non-Interfering Blocking Agent for Homogeneous Biosensor Development

et al. 2013

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Abstract.Many homogeneous assays are complicated by the adsorption of probe molecules by the surface of reaction vessels, which are often made of polypropylene or polystyrene-based plastics. To solve this problem, many protein and surfactant-based blocking agents are used. However, these blockers may interfere with intended assays by sequestering transition metal ions, inducing protein denaturing, generating air bubbles or making pores in membranes. Coating surfaces with polyethylene glycol (PEG) through covalent linkages have been proven to be an effective method to minimize protein adsorption.However, this method is more difficult to apply on plastic surfaces and is quite expensive. While unmodified PEG is often considered as a non-adsorbing polymer, in this Technical Note, we report that PEG at very low ppm can still effectively block plastic surfaces. This method works for DNA, protein and liposome-based assays as long as the molecular weight of PEG is larger than 2000. PEG works due to multivalent hydrophobic interaction from its repeating methylene units. This Technical Note will not only facilitate biosensor development, but also enhance our understanding of the interaction between various molecules and plastic surfaces.3

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DNA-Mediated Homogeneous Binding Assays for Nucleic Acids and Proteins

Cited by 387 publications

References 315 publications

Aptamer-based biosensors for biomedical diagnostics

Aptamer-based biosensors for biomedical diagnostics

Homogeneous Protein Analysis by Magnetic Core–Shell Nanorod Probes

Parts-per-Million of Polyethylene Glycol as a Non-Interfering Blocking Agent for Homogeneous Biosensor Development

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