DNA marking of the rate of maturation of gonads in female sterlet x beluga (A. ruthenus x H. huso) hybrid under conditions of recirculation systems

Abstract

To create a theoretical basis for the development of new technologies for the formation of highly productive sturgeon herds, work has begun on conducting research on DNA - markers associated with economically useful traits. At the first stage of the work, polymorphic regions of the mitochondrial DNA (mtDNA) D-loop were investigated in order to search for promising molecular genetic markers associated with the production properties of sturgeon hybrids. Along with point polymorphisms in mtDNA hybrids, variability in the length of the D-loop was observed, as well as the presence of heteroplasmy in length. The length variability of the D-loop is due to the presence of tandem repeating units in multiples of 80 base pairs (bp). Using Fisher’s exact test, it was shown that the proportion of individuals with four tandem repeating units of 80 bp eachsignificantly higher (p = 0.030) in the group of highly productive hybrids. The obtained data suggest that such a trait as the accelerated maturation of female hybrids (A. ruthenus x H. huso) grown in a closed water supply can be associated with the mitochondrial DNA haplotype, in the D-loop of which there are fourrepeating units.