DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae)

Malausa, Thibaut; Fenis, A.; Warot, Sylvie; Germain, J. F.; Ris, Nicolas; Prado, Ernesto; Botton, Marcos; Vanlerberghe‐Masutti, Flavie; Sforza, R.; Cruaud, Corinne; Couloux, Arnaud; Kreiter, Philippe

doi:10.1111/j.1439-0418.2009.01495.x

Cited by 87 publications

(134 citation statements)

References 37 publications

(77 reference statements)

Supporting

Mentioning

126

Contrasting

Unclassified

Order By: Relevance

“…The sequence comparison, after alignment (Fig 4 and 5), reveals the extent of divergence corresponding to interspecific variations in previous studies (Malausa et al 2011;Park et al 2011). At COI, the divergence was >7% between D. lavandulae and D. multivorus and >9% between D. lavandulae and the Trionymus species.…”

Section: Methodsmentioning

confidence: 99%

“…The third one, hereafter referred to as COI, is located in the mitochondrial region of Cytochrome Oxidase Subunit I. For COI, we amplified three overlapping fragments previously used in several molecular studies on Pseudococcidae (Malausa et al, 2011;Park et al, 2011;Abd-Rabou et al, 2012), covering in total ~1050 bp. The sequences of the primers used to amplify each region are provided in Table 1. PCR were performed using the Qiagen Multiplex PCR kit (QIAGEN, Hilden, Germany), in a reaction volume of 25 µL.…”

Section: Methodsmentioning

confidence: 99%

“…The DNA voucher specimens plus other preserved specimens were slide-mounted using the method described in Malausa et al (2011). The illustration ( The DNA characterization was performed on 5 specimens of D. lavandulae.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

A new species of Dysmicoccus damaging lavender in French Provence (Hemiptera, Sternorrhyncha, Pseudococcidae)

Germain

Matile-Ferrero

Kaydan

et al. 2015

Zootaxa

Self Cite

View full text Add to dashboard Cite

RésuméUne nouvelle espèce de Dysmicoccus nuisible à la lavande en Provence (France) (Hemiptera, Sternorrhyncha, Pseudococcidae). Dysmicoccus lavandulae Germain, Matile-Ferrero & Williams n. sp. est décrite et illustrée. Ses séquences ADN sont présentées. L'espèce vit sur Lavandula x intermedia cultivée pour la production d'essence de lavande en Provence. La liste des espèces de pseudococcines vivant sur les lavandes spontanées en France est dressée. Le statut des 2 genres voisins Trionymus Berg et Dysmicoccus Ferris est discuté. AbstractDysmicoccus lavandulae Germain, Matile-Ferrero & Williams n. sp. is described and illustrated, and its DNA sequences given. The species lives on Lavandula x intermedia, the cultivated form of lavender grown in the French region of Provence. In addition the mealybug species recorded in France on indigenous lavender are listed. The status of two related genera, Trionymus Berg and Dysmicoccus Ferris, is discussed.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A new species of Dysmicoccus damaging lavender in French Provence (Hemiptera, Sternorrhyncha, Pseudococcidae)

Germain

Matile-Ferrero

Kaydan

et al. 2015

Zootaxa

Self Cite

View full text Add to dashboard Cite

show abstract

“…Morphological identification of the species was made by J.-F. Germain (ANSES, Laboratoire National de la Protection des Végétaux, Montpellier, France). The species identification was ascertained by molecular characterization using a set of markers able to efficiently distinguish taxa within Pseudococcidae (22). Insects were maintained in darkness at 23°C (±2°C) in plastic jars whose lids had been perforated and covered with a 28 µm mesh tissue to enable air circulation.…”

Section: Methodsmentioning

confidence: 99%

Transmission of Six Ampeloviruses and Two Vitiviruses to Grapevine by Phenacoccus aceris

Maguet¹,

Beuve²,

Herrbach³

et al. 2012

Phytopathology®

View full text Add to dashboard Cite

Grapevine leafroll disease is caused by grapevine leafroll-associated viruses (GLRaVs). These viruses are common in vineyards worldwide and often associated with vitiviruses that are involved in the rugose wood complex of grapevine. Ten mealybug species are known as vectors of one or several of these grapevine viruses, including the apple mealybug Phenacoccus aceris which is widespread in Holarctic regions and able to transmit Grapevine leafroll-associated virus-1 and -3 (GLRaV-1 and -3). Our aim was to characterize the transmission features of leafroll viruses by Phenacoccus aceris in order to better understand the contribution of this mealybug to leafroll epidemics. Results showed that Phenacoccus aceris is able to transmit GLRaV-1, -3, -4, -5, -6, and -9 to grapevine but not GLRaV-7. This is the first report of GLRaV-6 transmission by a mealybug. Also, for the first time it was shown that Phenacoccus aceris could vector vitiviruses Grapevine virus A (GVA) and Grapevine virus B (GVB). First instar nymphs were the most efficient stage in transmitting GLRaV-1, -3, and GVA. This research sheds light on the transmission biology of grapevine viruses by Phenacoccus aceris and represents a step forward to leafroll disease management.

show abstract

“…Várias espécies do gênero Planococcus apresentam grande similaridade e podem diferenciar-se morfologicamente, ponderando os caracteres; contudo, em determinadas ocasiões, existe uma superposição dos dados, não sendo possível separá-las (COX; FREESTON, 1985). Os estudos moleculares desenvolvidos nos últimos anos têm demonstrado como uma nova ferramenta para a diferenciação das espécies deste gênero (MALAUSA et al, 2011;RUNG et al, 2008;CAVALIERI et al, 2008).…”

Section: Introductionunclassified

Especificidade alimentar: em busca de um caráter taxonômico para a diferenciação de duas espécies crípticas de cochonilhas do gênero Planococcus (Hemíptera: Pseudococcidae)

Sousa¹,

Souza²,

Santa-Cecília³

et al. 2012

Rev. Bras. Frutic.

View full text Add to dashboard Cite

fEEding spEcificitY: sEarching a taxonomic charactEr for thE diffErEntiation of tWo crYptic spEciEs of mEalYbugs of thE gEnus PLANOCOCUS (hEmiptEra: psEudococcidaE) abstract-The aim of this study was to investigate a possible host specificity of two cryptic species of mealybugs of the genus Planococcus, reflected in its development in fruits of cocoa, coffee and citrus. The mealybugs Planococcus minor were obtained from cocoa fruits (Theobroma cacao L. cv. Comum) and citrus mealybug, Planococcus citri, from coffee seedlings (Coffea arabica cv. Mundo Novo) and citrus fruits (Citrus sinensis L. Osbeck cv. Bahia). Both species were reared on pumpkins (Cucurbita maxima L.) under laboratory conditions. Coffee fruit clusters were kept into a Petri dish containing a 5 mm-agar layer sealed with PVC film. On citrus and cocoa fruits the insects were confined inside a clip-cage attached to the fruit.The tests were carried out in environmental chambers at 25 ± 1°C, 70 ± 10% RH and 12 hours photophase using 30 replications. For mealybugs from coffee, the coffee substrate provided the greatest female nymphal period (20.8 days) and longevity (31.7 days). For females from cocoa, the lowest nymphal period (21.1 days) and longevity (25.0 days) were promoted by cocoa substrate. For those from citrus, cocoa substrate promoted the lowest nymphal period (18.4 days) and the substrate citrus promoted the greatest longevity (32.0 days). The highest mortality (50%) occurred in nymphs derived from cocoa and citrus fruits, rearing in coffee and cocoa, and the smallest were derived from coffee fruits, regardless of the substrate on which they were rearing. The results did not show a clear host specificity despite of the mealybug P. minor showed a closer association with the cocoa and eventually coffee which would explain its greater frequency in cacao. On the other hand, the citrus mealybug, P. citri, did not show any specificity for the three tested hosts. index terms: Planococcus citri, Planococcus minor, host specificity, biology.1

show abstract

DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae)

Abstract: Mealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the

Cited by 87 publications

References 37 publications

A new species of Dysmicoccus damaging lavender in French Provence (Hemiptera, Sternorrhyncha, Pseudococcidae)

A new species of Dysmicoccus damaging lavender in French Provence (Hemiptera, Sternorrhyncha, Pseudococcidae)

Transmission of Six Ampeloviruses and Two Vitiviruses to Grapevine by Phenacoccus aceris

Especificidade alimentar: em busca de um caráter taxonômico para a diferenciação de duas espécies crípticas de cochonilhas do gênero Planococcus (Hemíptera: Pseudococcidae)

Contact Info

Product

Resources

About