DNA Marker Transmission and Linkage Analysis in Populations Derived from a Sugarcane (Saccharum spp.) x Erianthus arundinaceus Hybrid

Abstract

Introgression of Erianthus arundinaceus has been the focus of several sugarcane breeding programs in the world, because the species has desirable traits such as high biomass production, vigour, ratooning ability and good resistance to environmental stresses and disease. In this study four genetic maps were constructed for two intergeneric populations. The first population (BC1) was generated from a cross between an Erianthus/Saccharum hybrid YC96-40 and a commercial sugarcane variety CP84-1198. The second population (BC2) was generated from a cross between YCE01-116, a progeny of the BC1 cross and NJ57-416, a commercial sugarcane cultivar. Markers across both populations were generated using 35 AFLP and 23 SSR primer pairs. A total of 756 and 728 polymorphic markers were scored in the BC1 and BC2 populations, respectively. In the BC1 population, a higher proportion of markers was derived from the Erianthus ancestor than those from the Saccharum ancestor Badila. In the BC2 population, both the number and proportion of markers derived from Erianthus were approximately half of those in the BC1 population. Linkage analysis led to the construction of 38, 57, 36 and 47 linkage groups (LGs) for YC96-40, CP84-1198, YCE01-116, and NJ57-416, encompassing 116, 174, 97 and 159 markers (including single dose, double dose and bi-parental markers), respectively. These LGs could be further placed into four, five, five and six homology groups (HGs), respectively, based on information from multi-allelic SSR markers and repulsion phase linkages detected between LGs. Analysis of repulsion phase linkage indicated that Erianthus behaved like a true autopolyploid.

Molecular diversity and genetic structure of Saccharum complex accessions

“…Our result agrees with other studies that used AFLP [78,79], cpSSR [80], TRAP [21,30,59], SRAP [55] and SSR [3,81] markers. The introgression of alleles of the Erianthus genus in sugarcane breeding programs, mainly from E. arundinaceus, has been evaluated in recent years to increase adaptability, disease resistance, drought resistance and biomass production [82,83]. Despite this, further studies may be conducted to evaluate other regions of the genome closely related to the outstanding traits of the Erianthus genus.…”

Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions

“…(2 n = 20, 30, 40, 60) [ 7 ]. On the basis of the analysis of repulsion-phase linkage, Chen et al [ 8 ] reported that Erianthus behaves like a true autopolyploid. Of the seven species, E. arundinaceus and E. procerus have recently attracted attention for their use in Erianthus and sugarcane breeding to increase biomass production [ 9 , 10 ].…”

Chromosome transmission in BC4 progenies of intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus (Retz.) Jeswiet

“…and E . arundinaceus are polyploid plants with large genomes and many homologous sequences 24,25 . However, researchers can use the genome sequencing strategy for wheat, which is called “break up the whole into parts,” or “BAC BY BAC,” for Saccharum spp.…”