DNA marker-assisted evaluation of fruit firmness at harvest and post-harvest fruit softening in a diverse apple germplasm

Nybom, Hilde; Ahmadi-Afzadi, Masoud; Sehic, Jasna; Hertog, Maarten

doi:10.1007/s11295-012-0554-z

Cited by 39 publications

(71 citation statements)

References 32 publications

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“…However, for ‘Cox's Orange Pippin’ ( MdACS1‐1/2 ), our results were consistent with those of Nybom et al. (, ), but not with those of Sunako et al. (), who reported a MdACS1‐1/1 genotype.…”

Section: Resultssupporting

confidence: 66%

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Allelic polymorphism and inheritance of MdACS1 and MdACO1 genes in apple (Malus × domestica Borkh.)

Maric¹,

Lukić²

2013

Plant Breeding

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The allelic polymorphism and inheritance of MdACS1 and MdACO1 genes were analysed in 28 apple cultivars, which were derived from reciprocal crosses of the following parental pairs: ‘Golden Delicious’ × ‘James Grieve’, ‘Golden Delicious’ × ‘Jonathan’, ‘Cox's Orange Pippin’ × ‘Golden Delicious’, ‘Cox's Orange Pippin’ × ‘Jonathan’. Polymorphisms were detected by PCR and the use of two restriction enzymes (BamH1 and RsaI). In addition, new primers were designed for the further discrimination of MdACO1 alleles. Two alleles of MdACS1 gene (MdACS1‐1 and MdACS1‐2) and three alleles of MdACO1 gene (a, b and c) were detected. Cloning and sequencing of MdACO1 alleles confirmed a high conservation and some differences within the coding regions and helped to reconcile between different numbering systems. Observed segregations confirmed that alleles a, b and c belonged to the MdACO1 gene. It is apparent that polymorphisms within the MdACS1 and MdACO1 genes could aid cultivar genotyping and identification and, furthermore, that the MdACS1‐2/2 genotype is correlated with a long apple storage life.

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Section: Resultssupporting

confidence: 66%

“…(), Zhu and Barritt () and Nybom et al. (, ). However, for ‘Cox's Orange Pippin’ ( MdACS1‐1/2 ), our results were consistent with those of Nybom et al.…”

Section: Resultsmentioning

confidence: 99%

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Allelic polymorphism and inheritance of MdACS1 and MdACO1 genes in apple (Malus × domestica Borkh.)

Maric¹,

Lukić²

2013

Plant Breeding

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“…Weak correlations observed between harvest time and ADM values for the OA (r = −0.39, P < 0.001) and accessions (r = −0.37, P < 0.001) were consistent with the weak negative correlation between harvest time and storability found in a bi-parental population and genetic resources (Nybom et al, 2013). However, our GWAS for harvest time did not identify any significant SNPs (data not shown).…”

Section: Relationship Between Harvest Time Adm and Genotypes Of Md-psupporting

confidence: 79%

Identification of QTLs for Flesh Mealiness in Apple (<i>Malus</i> × <i>domestica</i> Borkh.)

Moriya¹,

Kunihisa²,

Okada³

et al. 2017

Hortic J

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During apple (Malus × domestica Borkh.) storage, a loss in fruit firmness can occur. This is frequently associated with mealiness, an undesirable trait. There have been studies, such as phenotypic analyses and transcriptomics, as well as others employing a transgenic approach, focusing on this trait. Certain genetic approaches, such as quantitative trait loci (QTL) approach, however, have not been attempted. In this study, to identify and characterize QTLs controlling flesh mealiness and to investigate their application in apple breeding, we performed classical QTL mapping based on a bi-parental population and a genome-wide association study (GWAS) of mealiness. Phenotypic data for mealiness allowed us to identify two QTLs in the bi-parental family located on linkage group 10. The GWAS discovered significant marker-trait associations on chromosomes 2, 9, and 10. The MdPG1 locus, located on chromosome 10, was identified as the locus with the strongest significance by both QTL mapping and GWAS, suggesting its primary contribution to flesh mealiness. Using a tri-allelic simple sequence repeat marker, Md-PG1 SSR 10kd, 10 kb downstream of the MdPG1 coding sequence, we divided apple accessions into six groups based on their genotypes. Among the six groups, the Md-PG1 SSR 10kd genotype "2/2" had the least mealy phenotype.

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“…Several studies confirm its role in the fruit storage pro cess (McQueen Mason and Cosgrove, 1994;Sharova, 2007). In addition, the authors find the dependence of the fruit's firmness on an allelic gene variant in their works (Costa et al, 2008, Nybom et al, 2012. How ever, the previous studies were all focused only on vari eties.…”

Section: Discussionmentioning

confidence: 82%

Polymorphism of the Md-Exp7 gene for the biosynthesis of expansin in wild species of the genus Malus Mill.

Savel’ev

Shamshin

Савельева

et al. 2015

Russ J Genet Appl Res

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Molecular genetic analysis revealed a polymorphism for the Md Exp7 gene that controls the bio synthesis of expansin in wild species of the genus Malus. Eight allelic forms of the gene were identified, four of which were unique. The regularities in the allele distribution in 37 genotypes were found to be in accor dance with their systematic positions. The trait was found to be broadly diverse both among the sections of the genus and within a particular section. Variants of the gene were identified in representatives of one species.

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DNA marker-assisted evaluation of fruit firmness at harvest and post-harvest fruit softening in a diverse apple germplasm

Cited by 39 publications

References 32 publications

Allelic polymorphism and inheritance of MdACS1 and MdACO1 genes in apple (Malus × domestica Borkh.)

Allelic polymorphism and inheritance of MdACS1 and MdACO1 genes in apple (Malus × domestica Borkh.)

Identification of QTLs for Flesh Mealiness in Apple (<i>Malus</i> × <i>domestica</i> Borkh.)

Polymorphism of the Md-Exp7 gene for the biosynthesis of expansin in wild species of the genus Malus Mill.

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