“…[42][43][44][45][46][47][48] Finally, sequence information may even be obtained from stretched molecules through restriction site mapping or motif mapping using hybridized probes. 19,20,49 In addition to stretching via hydrodynamic forces, channels, planar slits, and pillar arrays, fabricated into glass or silicon wafers can also be used to study the mechanical dynamics of a DNA molecule by employing entropic forces that arise due to confinement. To describe the origin of the confinement-induced forces that enable single molecule control and visualization, it is useful to compare the size of a DNA strand with the dimensions of nanofabricated structures.…”