DNA maintenance methylation enzyme Dnmt1 in satellite cells is essential for muscle regeneration

Uhrf1 governs the proliferation and differentiation of muscle satellite cells

“…Considering the enrichment of GO terms such as “DNA repair” in downregulated genes in mutant myogenic cells, the genome instability seems to be one of the mechanisms for explaining the phenotype of mutant myogenic cells. In addition, we found a number of phenotypes in Uhrf1 -deficient myogenic cells overlapped with Dnmt1 -deficient ones ( Iio et al., 2021 ). Further efforts to perform the integrated analysis of omics data, including histone modifications, of Uhrf1 - and Dnmt1 -deficient myogenic cells may help understand the different roles between Uhrf1 and Dnmt1 for regulating satellite cells.…”

“…Further, ablation of Uhrf1 in satellite cells led to impaired muscle regeneration, similar to Dnmt1- ( Iio et al., 2021 ) or Dnmt3a-deficient mice ( Naito et al., 2016 ). The marked loss of EdU incorporation indicated a remarkable reduction in the number of Uhrf1 -deficient myogenic cells in vitro and in vivo at 28 dpi.…”

“…Deleting DNMT3a in satellite cells led to loss of proliferation with increased expression of the Cdkn1c ( Naito et al., 2016 ) or the Gdf5 gene ( Hatazawa et al., 2018 ). Further, we reported that deletion of Dnmt1 in satellite cells impaired muscle regeneration in vivo, resulting in a reduced number of myogenic cells in vitro ( Iio et al., 2021 ). Thus, identification of epigenetic regulator is important to understand the mechanisms by which DNA methylation governs the proliferation and differentiation of satellite cells.…”

Fetal programming effect of rumen-protected methionine on primiparous Angus × Simmental offspring’s performance and skeletal muscle gene expression

“…During this study, young men who were exposed to a high-fat overfeeding diet for 5 d had an upregulation in DNMT1 and DNMT3a from skeletal muscle, suggesting that expression of DNMT1 could be altered in growing and mature mammals by the diet consumed ( Jacobsen et al, 2012 ). In addition, a recent experiment conducted by Iio et al (2021) found a novel function of DNMT1 during muscle regeneration in mature male mice. These results suggest that DNMT1 may be involved in other methylation processes in addition to cell division.…”