DNA/lysozyme binding propensity and nuclease properties of benzimidazole/2,2′-bipyridine based binuclear ternary transition metal complexes

Mansour, Ahmed M.; Ragab, M. T.

doi:10.1039/c9ra07188a

Cited by 15 publications

(7 citation statements)

References 53 publications

(44 reference statements)

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“…Additionally, DNA intercalation was studied by classic competitive binding assays with ethidium bromide . Most coordination compounds can intercalate DNA with K app ≈ 10 5 M, and the values obtained correlate with those obtained for coordination compounds of copper with anticancer activity . Expectedly, organic ligands do not intercalate DNA, except for cytotoxic ligand 16 (Table S25 and Figures S35–S37).…”

Section: Resultsmentioning

confidence: 59%

“…42 Most coordination compounds can intercalate DNA with K app ≈ 10 5 M, and the values obtained correlate with those obtained for coordination compounds of copper with anticancer activity. 43 Expectedly, organic ligands do not intercalate DNA, except for cytotoxic ligand 16 (Table S25 and Figures S35−S37). However, a lack of correlation between the ability to intercalate DNA and cytotoxicity makes the DNA intercalation ability noncritical for the cytotoxicity of studied ligands and coordination compounds.…”

Section: ■ Introductionmentioning

confidence: 99%

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Novel Copper-Containing Cytotoxic Agents Based on 2-Thioxoimidazolones

et al. 2020

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A series of 73 ligands and 73 of their Cu+2 and Cu+1 copper complexes with different geometries, oxidation states of the metal, and redox activities were synthesized and characterized. The aim of the study was to establish the structure–activity relationship within a series of analogues with different substituents at the N(3) position, which govern the redox potentials of the Cu+2/Cu+1 redox couples, ROS generation ability, and intracellular accumulation. Possible cytotoxicity mechanisms, such as DNA damage, DNA intercalation, telomerase inhibition, and apoptosis induction, have been investigated. ROS formation in MCF-7 cells and three-dimensional (3D) spheroids was proven using the Pt-nanoelectrode. Drug accumulation and ROS formation at 40–60 μm spheroid depths were found to be the key factors for the drug efficacy in the 3D tumor model, governed by the Cu+2/Cu+1 redox potential. A nontoxic in vivo single-dose evaluation for two binuclear mixed-valence Cu+1/Cu+2 redox-active coordination compounds, 72k and 61k, was conducted.

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Section: Resultsmentioning

confidence: 59%

Section: ■ Introductionmentioning

confidence: 99%

Novel Copper-Containing Cytotoxic Agents Based on 2-Thioxoimidazolones

et al. 2020

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“…In 1952, the first reported studies regarding the biological activity of cobalt complexes examined the toxicity of the optical isomers of [Co(en) 3 ](NO 3 ) 3 (en = ethylenediamine) [ 3 ]. Since then, many cobalt complexes of biological interest have been reported with the majority of them showing antiproliferative [ [4] , [5] , [6] , [7] ], antimicrobial [ [8] , [9] , [10] ], antifungal [ 11 , 12 ], antiviral [ 13 , 14 ], and antioxidant [ [15] , [16] , [17] ] activity.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, structural determination, in vitro and in silico biological evaluation of divalent or trivalent cobalt complexes with indomethacin

Perontsis

Geromichalou

Perdih

et al. 2020

Journal of Inorganic Biochemistry

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The interaction of cobalt chloride with the non-steroidal anti-inflammatory drug indomethacin (Hindo) led to the formation of the polymeric complex [Co(indo-O) 2 (H 2 O) 2 (μ-Cl)] n ·n(MeOH·H 2 O) bearing one chlorido bridge between the cobalt atoms. The presence of the nitrogen-donor co-ligands 2,2′-bipyridine (bipy), 2,2′-bipyridylamine (bipyam), 1,10-phenanthroline (phen) or 1 H -imidazole (Himi) resulted in the isolation of complexes [Co 2 (μ-indo-O,O′) 2 (indo-O) 2 (bipy) 2 (μ-H 2 O)]·3.3MeOH, [Co(indo-O,O′) 2 (bipyam)]·0.9MeOH·0.2H 2 O, [Co(indo-O,O′) 2 (phen)] ( 4 ) and [Co(indo-O) 2 (Himi) 2 ] ( 5 ), respectively, where the indomethacin ligands were coordinated in diverse manners. The study of the affinity of the complexes for calf-thymus DNA revealed their intercalation between the DNA-bases. The binding of the complexes to albumins was also examined and the corresponding binding constants and binding subdomain were determined. The free radical scavenging activity of the compounds was evaluated towards 1,1-diphenyl-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid). Molecular modeling calculations may usually provide a molecular basis for the understanding of both the impairment of DNA by its binding with the studied complexes and the ability of these compounds to transportation through serum albumin proteins. This study can provide information for the elucidation of the mechanism of action of the compounds in a molecular level.

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“…Form I, one of the three bands formed as a result of agarose gel electrophoresis, moves faster than Form II and Form III. This is due to the large load density and low volume (Mansour and Ragab 2019 ). According to Sohrabijam et al ( 2017 ), they investigated the effect of chitosan coated superparamagnetic nanoparticles on DNA molecules.…”

Section: Resultsmentioning

confidence: 99%

A comparative study of iron nanoflower and nanocube in terms of antibacterial properties

et al. 2023

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It is known that heavy metal containing nanomaterials can easily prevent the formation of microbial cultures. The emergence of new generation epidemic diseases in the last 2 years has increased the importance of both personal and environmental hygiene. For this reason, in addition to preventing the spread of diseases, studies on alternative disinfectant substances are also carried out. In this study, the antibacterial activity of nanoflower and nanocube, which are easily synthesized and nanoparticle species containing iron, were compared. The antioxidant abilities of new synthesized NF@FeO(OH) and NC@α-Fe 2 O 3 were tested by DPPH scavenging activity assay. The highest DPPH inhibition was achieved with NC@α-Fe 2 O 3 as 71.30% at 200 mg/L. NF@FeO(OH) and NC@α-Fe 2 O 3 demonstrated excellent DNA cleavage ability. The antimicrobial capabilities of NF@FeO(OH) and NC@α-Fe 2 O 3 were analyzed with micro dilution procedure. In 500 mg/L, the antimicrobial activity was 100%. In addition to these, the biofilm inhibition of NF@FeO(OH) and NC@α-Fe 2 O 3 were investigated against S. aureus and P. aeruginosa and it was found that they showed significant antibiofilm inhibition. It is suggested that additional studies can be continued to be developed and used as an antibacterial according to the results of the nanoparticles after various toxicological test systems.

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DNA/lysozyme binding propensity and nuclease properties of benzimidazole/2,2′-bipyridine based binuclear ternary transition metal complexes

Abstract: The binuclear ternary transition metal complexes have the ability to bind to lysozyme and DNA as well as DNA cleavage. Complexes exhibited cytotoxicity to only the cancerous cells (HepG-2) and is safe to the non-malignant HEK-293.

Cited by 15 publications

References 53 publications

Novel Copper-Containing Cytotoxic Agents Based on 2-Thioxoimidazolones

Novel Copper-Containing Cytotoxic Agents Based on 2-Thioxoimidazolones

Synthesis, structural determination, in vitro and in silico biological evaluation of divalent or trivalent cobalt complexes with indomethacin

A comparative study of iron nanoflower and nanocube in terms of antibacterial properties

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