DNA investigative lead development from blood and saliva samples in less than two hours using the RapidHIT™ Human DNA Identification System

Assessment of Four Polymerases for Low Volume, Fast PCR Amplification of STR Loci for DNA Reference Samples

“…More recently, rapid DNA testing has been achieved in less than two hours from start to finish using a single instrument, and has demonstrated its suitability for investigative leads when time is a crucial factor [14,15], though it is not a cost effective means of testing for high-throughput laboratories. For high-throughput laboratories, time and cost effectiveness are critical, and often, the means to achieving a reduction in processing time includes added costs (e.g., use of fast thermal cyclers and fast polymerases).…”

Evaluation of the RapidHIT™ 200 System: A comparative study of its performance with Maxwell ® DNA IQ™/Identifiler ® Plus/ABI 3500xL workflow

“…These are, however, attempts which accelerate individual steps of the DNA analysis process. The RapidHIT TM 200 from IntegenX Inc., in contrast, offers an integrated single-instrument platform that combines sample DNA extraction, STR-PCR amplification, amplicon separation and detection, and DNA profile analysis, all within a relatively short duration of about 2 h [16][17][18][19][20][21][22].…”

“…The instrument has been manufacturervalidated [16][17][18] and tested by various groups [19][20][21][22] for the processing of reference buccal samples. In the present study, we seek to evaluate the performance of the RapidHIT TM 200 to generate DNA profiles from samples routinely encountered in crime casework.…”

Microchip‐based forensic short tandem repeat genotyping

“…The RapidHIT system is capable of producing STR profiles in less than 90 min with several advantages of full integration, automation, simplification, and no human intervention. The RapidHIT system contains two cartridges with eight slots (for five samples, one positive control, one negative control, and one ladder), a sample pretreatment system for bead‐based DNA isolation, a PCR amplification system with use of GlobalFiler Express Kit or PowerPlex 16 HS Kit, and a CE system using a fused‐silica capillary array .…”