ha human, the gone coding for apolipoprotein A-I (apo A-I), a protein of the plasma lipid transport system, is expressed only in the liver and the intestine, A naturally occurring A to G substitution in the promoter at position -78 was shown to be associated with high density lip0proteins (HDL) in females. We have studied the effect of this mutation on promoter activity using various lengths of protnoter sequences and the CAT reporter $ene system, Transient expression studies after introduction of these constructs into Hop 3B cells r~vc.aled that in the region spanning -330 to + I of the promoter an A to G substitution increases the activity approximately twofold, On the other hand, when further upstream r~gion (-1469 to +397) is also included, th~ promoter activity seems comparable in both alleles, Our results show how minimal sequence variations can affect the in vitro a~mlysis or'promoter activity.