DNA interaction and cytotoxic activities of square planar platinum(II) complexes with N, S-donor ligands

Patel, Mohan N.; Patel, Chintan R.; Joshi, Hardik; Thakor, Khyati P.

doi:10.1016/j.saa.2014.02.053

Cited by 20 publications

(6 citation statements)

References 37 publications

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“…The change of the contour length of DNA in presence of the complexes provide relevant information about the binding. It is well known that the intercalation process increases the viscosity [72], while the covalent binding decreases slightly it [154]. Any modification of DNA viscosity was observed with 1[C,NH] and 2[C,NMe], supporting the absence of interaction with DNA.…”

Section: Viscositymentioning

confidence: 81%

Interacción de ARN con doxorrubicina: Influencia del ligando y del metal en la actividad biológica de complejos organometálicos

Antolín¹

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Section: Viscositymentioning

confidence: 81%

Interacción de ARN con doxorrubicina: Influencia del ligando y del metal en la actividad biológica de complejos organometálicos

Antolín¹

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“…Viscosity values were calculated from the observed flow time of DNA‐containing solutions ( t ) corrected for that of the buffer alone ( t 0 ), η = ( t − t 0 )/ t 0 . [ 40 ]…”

Section: Methodsmentioning

confidence: 99%

Organoruthenium (II) complexes featuring pyrazole‐linked thiosemicarbazone ligands: Synthesis, DNA/BSA interactions, molecular docking, and cytotoxicity studies

Khanvilkar

Dash

Banerjee

et al. 2021

Applied Organom Chemis

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A series of pyrazol-derived thiosemicarbazone ligands (L1-L4) were synthesized and reacted with [Ru(p-cymene)(μ-Cl)Cl] 2 to yield a series of "piano-stool"-type binuclear ruthenium (II)-arene-thiosemicarbazone complexes (C1-C8) of the general type [(Ru(η 6 -p-cym)L) 2 (μ-im/azpy)] Cl 1-2 (L = diphenylpyrazole thiosemicarbazone; cym = p-cymene; im = imidazole; azpy = 4,4 0 -azopyridine). The thiosemicarbazone ligands act as N and S donors binding to the Ru(II) center via the imine nitrogen and the thione sulfur atoms. The complexes were characterized by NMR, FTIR, UV-Vis spectroscopy, and ESI + mass spectrometry. The binding of the complexes to calf thymus deoxyribonucleic acid (CT-DNA) and bovine serum albumin (BSA) was evaluated, and it has been established that the binuclear complexes have good binding efficacies with DNA (K b = 10 4 -10 5 M À1 ) and BSA (K a = 10 5 -10 6 M À1 ). This is attributed to the arene moieties present in the ligands of the complexes that can have hydrophobic interactions with DNA/BSA. Ethidium bromide (EB) displacement studies and DNA viscosity measurements revealed intercalative interaction of the complexes with DNA. Static interaction of the complexes with BSA was revealed by fluorescence quenching studies. Molecular docking studies confirmed base stacking, H-bonding, and hydrophobic interactions with the biomolecules. In vitro antiproliferative studies of the complexes affirmed that the complexes are cytotoxic towards the HeLa (human cervical cancer) cell line with IC 50 values in range of 17.3-41.3 μM. K E Y W O R D S binuclear ruthenium (II) complexes, DNA/BSA binding interactions, HeLa human cervical carcinoma, pyrazole-derived thiosemicarbazone

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“…In vitro cytotoxicity was performed according to our previously published literature . Data were analyzed by simple logit method to determine the LC 50 values, in which the log of concentration of samples were plotted against percentage of mortality of nauplii.…”

Section: Methodsmentioning

confidence: 99%

Synthesis, characterization and biological applications of some substituted pyrazoline based palladium (II) compounds

Thakor

Lunagariya

Bhatt

et al. 2018

Applied Organom Chemis

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Palladium (II) complexes of the type [PdLCl 2 ] (where L = substituted pyrazoline ligands) have been synthesized. The metal complexes (5a-5f) have been characterized by various spectroscopic and analytical techniques like 1 H-NMR, 13 C-NMR, LC-MS, IR, Energy-dispersive X-ray (EDX), electronic spectroscopy, thermogravimetric analysis (TGA), elemental analysis and conductance measurement. Complexes to herring sperm DNA (HS-DNA) binding has been explored by absorption titration and binding constant (K b ) as well as Gibb's free energy have been evaluated. Complex 5b shows the highest binding constant value. So, thermodynamic parameters of 5b-HS DNA complex at different temperature have been evaluated. Also, Viscosity measurement and molecular modeling studies have been performed to know the binding mode of complexes. Based on the observations, an intercalative binding mode of DNA has been proposed. Further confirmation of intercalative mode of DNA binding has been taken by fluorescence spectroscopy and results are in a good agreement with absorption titration, viscosity measurement and molecular modeling data. Antibacterial activity of the complexes has been screened against pathogenic bacteria such as S. aureus, E. coli, B. subtilis, S. marcescens and P. aeruginosa. Cytotoxicity is performed on brine shrimp and S. pombe. Gel electrophoresis assay demonstrates that all the complexes can cleave the pUC19 plasmid DNA. Anti-tuberculosis activity has been carried out using mycobacterium tuberculosis H 37 Rv bacteria by L.J. Medium conventional method.

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DNA interaction and cytotoxic activities of square planar platinum(II) complexes with N, S-donor ligands

Cited by 20 publications

References 37 publications

Interacción de ARN con doxorrubicina: Influencia del ligando y del metal en la actividad biológica de complejos organometálicos

Interacción de ARN con doxorrubicina: Influencia del ligando y del metal en la actividad biológica de complejos organometálicos

Organoruthenium (II) complexes featuring pyrazole‐linked thiosemicarbazone ligands: Synthesis, DNA/BSA interactions, molecular docking, and cytotoxicity studies

Synthesis, characterization and biological applications of some substituted pyrazoline based palladium (II) compounds

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