DNA‐Inspired Hierarchical Polymer Design: Electrostatics and Hydrogen Bonding in Concert

Hemp, Sean T.; Long, Timothy Edward

doi:10.1002/mabi.201100355

Cited by 22 publications

(16 citation statements)

References 85 publications

(90 reference statements)

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“…It may have such effects also in the PRT assay. Even though most three-dimensional DNA structures related to histones would be resolved during DNA purification procedures, supramolecular assembly of the DNA structure resulting from various noncovalent interactions such as hydrogen bonding, electrostatics, and π−π stacking [33] could affect the final PRT results. To test for this possibility, we added an extra-denaturation procedure to the PRT assay (denaturing PRT), as in our mrcPCR assay.…”

Section: Resultsmentioning

confidence: 99%

Simple and Versatile Molecular Method of Copy-Number Measurement Using Cloned Competitors

et al. 2013

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Variations and alterations of copy numbers (CNVs and CNAs) carry disease susceptibility and drug responsiveness implications. Although there are many molecular methods to measure copy numbers, sensitivity, reproducibility, cost, and time issues remain. In the present study, we were able to solve those problems utilizing our modified real competitive PCR method with cloned competitors (mrcPCR). First, the mrcPCR for ERBB2 copy number was established, and the results were comparable to current standard methods but with a shorter assay time and a lower cost. Second, the mrcPCR assays for 24 drug-target genes were established, and the results in a panel of NCI-60 cells were comparable to those from real-time PCR and microarray. Third, the mrcPCR results for FCGR3A and the FCGR3B CNVs were comparable to those by the paralog ratio test (PRT), but without PRT's limitations. These results suggest that mrcPCR is comparable to the currently available standard or the most sensitive methods. In addition, mrcPCR would be invaluable for measurement of CNVs in genes with variants of similar structures, because combination of the other methods is not necessary, along with its other advantages such as short assay time, small sample amount requirement, and applicability to all sequences and genes.

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Section: Resultsmentioning

confidence: 99%

Simple and Versatile Molecular Method of Copy-Number Measurement Using Cloned Competitors

et al. 2013

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“…Much work in SRP research makes use of controlled radical and other living type reactions [9][10][11][12][13][14] nucleobase polymers [15,16], and information containing polymers [17,18].…”

Section: Background and Motivationmentioning

confidence: 99%

Simultaneous Monitoring of the Effects of Multiple Ionic Strengths on Properties of Copolymeric Polyelectrolytes during Their Synthesis

Zhu

Drenski³

et al. 2017

Processes

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Abstract:A new Automatic Continuous Online Monitoring of Polymerization reactions (ACOMP) system has been developed with multiple light scattering and viscosity detection stages in serial flow, where solution conditions are different at each stage. Solution conditions can include ionic strength (IS), pH, surfactants, concentration, and other factors. This allows behavior of a polymer under simultaneous, varying solution conditions to be monitored at each instant of its synthesis. The system can potentially be used for realtime formulation, where a solution formulation is built up additively in successive stages. It can also monitor the effect of solution conditions on stimuli responsive polymers, as their responsiveness changes during synthesis. In this first work, the new ACOMP system monitored light scattering and reduced viscosity properties of copolymeric polyelectrolytes under various IS during synthesis. Aqueous copolymerization of acrylamide (Am) and styrene sulfonate (SS) was used. Polyelectrolytes in solution expand as IS decreases, leading to increased intrinsic viscosity (η) and suppression of light scattering intensity due to electrostatically enhanced second and third virial coefficients, A 2 and A 3 . At a fixed IS, the same effects occur if polyelectrolyte linear charge density (ξ) increases. This work presents polyelectrolyte response to a series of IS and changing ξ during chemical synthesis.Keywords: ACOMP; online monitoring; copolymeric polyelectrolytes; light scattering; viscosity Background and MotivationThis work introduces a new version of the Automatic Continuous Online Monitoring of Polymerization reactions (ACOMP) system ("second generation ACOMP") whose aim is to monitor the onset and evolution of stimuli responsive behavior, under multiple simultaneous solution conditions, during the synthesis of stimuli responsive polymers (SRP). SRP is a vast area of modern polymer science and engineering, aimed at producing polymers that can respond to such stimuli as temperature, radiation, and solution conditions such as pH, ionic strength, polymer concentration, presence of such agents as surfactants, nanoparticles, hydrophobic species, etc. The types of responses that can occur in response to these stimuli include coil/globule phase transitions, polymer coil expansion or shrinkage, micellization, aggregation, and other forms of spontaneous self-assembly.These next-generation materials are expected to have applications in medicine, sensors, self-healing materials, and environmental remediation [1][2][3][4][5]. Hydrogels ofpoly(N-isopropylacrylamide), for example, have a lower critical solution temperature (LCST), near body temperature, which makes it a candidate for drug delivery applications in which the NIPAM-based polymer releases its medical

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“…Orthogonal functionalization through electrostatic interactions and complementary hydrogen bonding allows modification of polymer properties significantly and the design of materials with properties that are required for biomedical applications. 65 Another elegant example reports on the use of RAFT polymerization to create self-complementary nucleobase-functionalized ABC triblock copolymers with fully acrylic backbones that self-assemble into well-defined lamellar microphase-separated morphologies ( Fig. 4A and B).…”

Section: Supramolecular Biomaterials Inspired By Nucleobase Self-assementioning

confidence: 99%

Advances in the Development of Supramolecular Polymeric Biomaterials

Goor

Dankers²

2017

Comprehensive Supramolecular Chemistry II

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DNA‐Inspired Hierarchical Polymer Design: Electrostatics and Hydrogen Bonding in Concert

Cited by 22 publications

References 85 publications

Simple and Versatile Molecular Method of Copy-Number Measurement Using Cloned Competitors

Simple and Versatile Molecular Method of Copy-Number Measurement Using Cloned Competitors

Simultaneous Monitoring of the Effects of Multiple Ionic Strengths on Properties of Copolymeric Polyelectrolytes during Their Synthesis

Advances in the Development of Supramolecular Polymeric Biomaterials

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