DNA in diploid and androgenetic amphibian hybrids

Moore, Betty C.

doi:10.1002/jmor.1051010203

Cited by 25 publications

(1 citation statement)

References 84 publications

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“…Following incubation, the tissues were fixed and processed for paraffin embedding by the method of Moore (14), with 2% MgC12 added to the original fixative to minimize the possible distortion of the segments by smooth muscle contraction (15) . 5-µ sections were decerated, hydrated, and covered with Kodak NTB-3 radioautographic emulsion .…”

Section: Radioautographic Studiesmentioning

confidence: 99%

Metabolic and Ultrastructural Changes in the Frog Ovarian Follicle in Response to Pituitary Stimulation

Anderson

Yatvin

1970

The Journal of Cell Biology

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Frog ovarian fragments were prevented from ovulating in vitro by the addition of actinomycin D up to 3 hr following pituitary stimulation ; but addition of Actinomycin D 6 hr after stimulation was far less effective . Puromycin, on the other hand, effectively inhibited ovulation when added as late as 6 hr after pituitary stimulation . Although actinomycin D reduced uptake of uridine-3H, and puromycin reduced uptake of leucine 8H and lysine-14C by pituitary-stimulated ovarian tissue minus oocytes (OTMO) in vitro, it was found that pituitary stimulation did not significantly increase uptake of these compounds by OTMO . Radioautographs of ovarian follicles fixed 6 hr after the addition of pituitary extract and uridine sH in vitro revealed increased RNA synthesis in the peritoneal surface epithelium, compared with unstimulated controls, while the ovarian sac epithelium showed no increase . Gross ultrastructural changes occurred in the peritoneal area of ovarian follicles following pituitary stimulation in vivo, including loss of collagen fibrils, and general disorganization of the connective tissue theca . Changes in the rough endoplasmic reticulum of the peritoneal epithelial cells, while frequently encountered, were less pronounced . None of these changes was observed in the ovarian sac area, or in the interfollicular region . The above data are consistent with the hypothesis that pituitary stimulation of the frog ovary results in increased synthesis of RNA and protein by the peritoneal epithelial cells, and that the protein may be collagenase .

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Section: Radioautographic Studiesmentioning

confidence: 99%

Metabolic and Ultrastructural Changes in the Frog Ovarian Follicle in Response to Pituitary Stimulation

Anderson

Yatvin

1970

The Journal of Cell Biology

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Nuclear transplantation and problems of specificity in developing embryos

Moore

1962

J. Cell. Comp. Physiol.

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Synthesis of desoxyribonucleic acid during amphibian embryogenesis at different temperatures

Løvtrup

1959

J. Exp. Zool.

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TITO FIGURES INTRODUCTIONNormal development of amphibian embryos is known to occur within a quite wide temperature interval -around 20°C (cf. Moore, '39). Within this range the morphological development is rather unaffected, although body size may be smaller and gill size larger at high temperatures (Chambers, '08; Doms, '15). This does not imply that the developmental processes are identical at all temperatures. On the contrary it has been possible to show that the supply of energy, and the utilization of energy sources is strongly influenced by temperature (Ldvtrup, '53b ; ' 5 9~) . This in turn influences the synthesis of enzymes in such a way that the maximum amount of enzymes is highest at intermediate temperatures, and decreases as the temperature approaches the limits of the range (Lrzlvtrup, '53c; '55; '59d). Retardation of morphological development may be observed at low temperatures (L@vtrup, '59d). Previous investigations are seen to comprise the following aspects of embryogenesis : Morphological development, energy utilization, and chemical diff ercntiation. I n so far as the content of desoxyribonucleic acid (DNA) may be taken to represent the number of cells, the present investigation of DNA synthesis a t different temperatures represents an analysis of the temperature effect on yet an other embryonic process, viz., cell division. 545 S#REN L~T R U P MATERIAL AND METHODAdult specimens of R a n n pla,tyrrh,ina (= R. temporaria) were collected in nature and allowed to mate in the laboratory. The eggs were placed in Erlenmeyer flasks (50-100 in each), and the flasks were partly left at room temperature (RT; about 20°C), and partly immersed in water baths at 10°C.The DNA content of the embryos was determined on acetone dried powders. The samples from the earliest stages contained 25 eggs or embryos, from the later stages the number was 5-10. The microbiological method developed by Hoff-Jgkgensen ('52; '54) was used for the analyses in the modification suggested by L@vtrup and Roos ( '57). Work on this method which has gone on f o r several years in my laboratory has shown that certain precautions are necessary if reliable results are to be obtained. The most important is probably that the pH and the bu-ffer capacity of the medium must be controlled very carefully. The difference in buffer capacity arising from diluting samples of varying DNA content to different degrees with water suffices to make the results incomparable. It is furthermore our experience that desoxyribonuclease alone cannot digest certain types of DNA sufficiently to make them available for the microorganism. For other types the treatment with this one enzyme may be enough. The additional use of snake venom has been suggested by various authors. We have found that, depending upon the circumstances, snake venom may either increase or decrease the yield, or be without any influence. The details of these investigations m7ill be published in a separate paper (LGvtrup and ROOS, '60). The method used in the present work may not be the ul...

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DNA in diploid and androgenetic amphibian hybrids

Cited by 25 publications

References 84 publications

Metabolic and Ultrastructural Changes in the Frog Ovarian Follicle in Response to Pituitary Stimulation

Metabolic and Ultrastructural Changes in the Frog Ovarian Follicle in Response to Pituitary Stimulation

Nuclear transplantation and problems of specificity in developing embryos

Synthesis of desoxyribonucleic acid during amphibian embryogenesis at different temperatures

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