DNA Hydrolysis by Homo- and Heteronuclear Cu(II)?Ni(II) Complexes of Two Diester-type Ligands

Saglam, Nagihan; Çolak, Ahmėt; Serbest, Kerim; Karaböcek, Serdar; Güner, Saadettin

doi:10.1007/s00706-004-0178-6

Cited by 7 publications

(7 citation statements)

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“…Several reports suggest that the possible reduction of Cu(II) to Cu(I) and followed by the redox cycling of the metal ion gives rise to the formation of reactive oxygen species, particularly production of either hydroxyl radical or singlet oxygen species in this process, are responsible for the DNA cleavage activity. 37,38,49,62 Similarly, in our experiment, when the hydroxyl radicals and singlet oxygen species were scavenged by potassium iodide and sodium azide, respectively, the nucleolytic activity of the peroxide treated complex in the plasmid DNA was completely abolished (Figure 10a). Superoxide radicals have generally a shorter half-life.…”

Section: Discussionsupporting

confidence: 69%

“…Finally, agarose gel electrophoresis with pUC19 plasmid DNA (500 ng) was performed (Figure 10) in the presence of different radical scavengers, sodium azide, potassium iodide, and catalase to confirm the production of reactive oxygen species in the reaction between Cu(II) complex (417 µM) and H 2 O 2 (550 µM). Sodium azide is a well-known singlet oxygen scavenger; potassium iodide can eliminate hydroxyl radicals, 49 and catalase can neutralize reactive hydrogen peroxide efficiently. As seen in Figure 10a, the reduced Cu(II) complex mediated plasmid DNA cleavages were completely protected in the presence of either of the two scavenging agents, KI and NaN 3 (compare the RF1 band intensities in lanes 4 and 5 with respect to lane 3).…”

Section: Resultsmentioning

confidence: 99%

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DNA Binding Ability and Hydrogen Peroxide Induced Nuclease Activity of a Novel Cu(II) Complex with Malonate as the Primary Ligand and Protonated 2-Amino-4-picoline as the Counterion

et al. 2010

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The DNA binding property of a Cu(II) complex, viz., [Cu(mal)(2)](picH)(2).2H(2)O, (mal)(2) = malonic acid, picH = protonated 2-amino-4-picoline, has been investigated in this study. The binding of this complex with plasmid and chromosomal DNA has been characterized by different biophysical techniques. From the absorption and fluorescence spectroscopic studies, it has been observed that the said copper complex binds strongly with pUC19 plasmid and CT DNA with a binding affinity of 2.368 x 10(3) and 4.0 x 10(3) M(-1), respectively, in 10 mM citrate-phosphate buffer, pH 7.4. Spectrofluorimetric studies reveal that the copper complex exhibits partial DNA intercalation as well as partial DNA minor groove binding properties. Consequently, in agarose gel electrophoresis study, it has been observed that the complex alone induces positive supercoiling in plasmid DNA while in the presence of H(2)O(2) it exhibits nuclease activity. The induction of the breakage in DNA backbone depends upon the relative concentrations of H(2)O(2) and copper complex followed by the time of incubation with DNA. Optical DNA melting study, isothermal titration calorimetry, and absorption spectroscopy have been used to characterize the nuclease activity of this complex in the presence of H(2)O(2). Further, (1)H NMR study indicates that Cu(II) in the complex is converted into the Cu(I) state by the reduction of H(2)O(2). Finally, agarose gel electrophoresis study with different radical scavengers concludes that the production of both hydroxyl radicals and reactive oxygen species is responsible for this nuclease activity.

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Section: Discussionsupporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

DNA Binding Ability and Hydrogen Peroxide Induced Nuclease Activity of a Novel Cu(II) Complex with Malonate as the Primary Ligand and Protonated 2-Amino-4-picoline as the Counterion

et al. 2010

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“…On the other hand, distasmycin at the same concentration and after irradiation at 365 nm for 1 h, did not show any significant cleavage of ccc-DNA. We further studied the reactive species that might be involved in the photocleavage by Ib using various reported radical scavengers 20 (Fig. 2b).…”

Section: Photoinduced Dna Cleavage and Mechanistic Characterizationmentioning

confidence: 99%

Synthesis of directly linked diazine isosteres of pyrrole-polyamide that photochemically cleave DNA

et al. 2012

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A distamycin model containing an isosteric diazine linked pyrrole has been designed and synthesized. The key steps of the synthesis involved the successful diazotization of the 4-amino-pyrrole derivatives to give the diazomium salts, which undergo coupling reactions with N-methylpyrrole to yield the directly linked diazine compounds. The amide isosteric-diazine pyrrole I demonstrated photo-induced DNA damage upon iradiation with UV light (365 nm). Spectrophotometric and mass spectrometric identification suggest that the azo-linkage in I did not dissociate during irradiation. Moreover, compound I produced DNase I footprints with the HexB DNA fragment at AT sites, as well as some other mixed sequences (5'-ATGTCG-3'), indicative of the additional role of the diazine-linkage for interaction at the duplex DNA.

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“…Recently, biological studies of oxime ligands and metal complexes gained importance. In order to examine the features of DNA cleaving and combining, many studies have been conducted about characterization and developing new compounds modified with organic and inorganic subtances for 30 years (5)(6)(7)(8)(9)(10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization of new homo and heteronuclear Schiff base copper(II) complexes

Topal

Karapinar

2018

Journal of the Turkish Chemical Society Section A: Chemistry

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This article describes the synthesis of isonitrosomethyl-p-tolyl ketone [HL] as the starting compound and then we prepared the new oxime ligand from the reactions of the amine materials with 1,2-diaminobenzene [H2L 1 ]. (1E, 2E, 1'E, 2'E)-3,3'-bis [(4-tolyl ketone)-(1,2 phenylene diimine)]. Acetaldehyde dioxime ligand was used to synthesize the metal complexes. The complexes were elucidated by FT-IR, elemental analysis, magnetic moment measurements, molar conductivity, mass spectra, and TGA studies. The free ligand was also described with its 13 C and 1 H-NMR spectra. For complexes using spectroscopic and stoichiometric data of the complexes showed that metal:ligand ratio of mononuclear copper(II) complex was found to be 1:1 while this ratio was 2:1 in homoand heterodinuclear copper(II) complex and 3:2 in the trinuclear copper(II) complex. All these data show us that the proposed structure is consistent with the obtained data.

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DNA Hydrolysis by Homo- and Heteronuclear Cu(II)?Ni(II) Complexes of Two Diester-type Ligands

Cited by 7 publications

References 0 publications

DNA Binding Ability and Hydrogen Peroxide Induced Nuclease Activity of a Novel Cu(II) Complex with Malonate as the Primary Ligand and Protonated 2-Amino-4-picoline as the Counterion

DNA Binding Ability and Hydrogen Peroxide Induced Nuclease Activity of a Novel Cu(II) Complex with Malonate as the Primary Ligand and Protonated 2-Amino-4-picoline as the Counterion

Synthesis of directly linked diazine isosteres of pyrrole-polyamide that photochemically cleave DNA

Synthesis, characterization of new homo and heteronuclear Schiff base copper(II) complexes

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