DNA Hydrogel Fiber with Self‐Entanglement Prepared by Using an Ionic Liquid

Lee, Chang Kee; Shin, Su Ryon; Lee, Sun Hee; Jeon, Ju‐Hong; So, Insuk; Kang, Tong Mook; Kim, Sun I.; Mun, Ji Young; Han, Sung-Sik; Spinks, Geoffrey M.; Wallace, Gordon G.; Kim, Seon Jeong

doi:10.1002/ange.200704600

Cited by 15 publications

(12 citation statements)

References 24 publications

(19 reference statements)

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“…DNA molecules can be designed and synthesized with multiple arms and complementary sticky ends. These branched DNA monomers include X-, Y- and T-shaped DNA [67–69], which can be tailored to form DNA hydrogel networks for specific biomedical applications, such as 3D cell culture, cell transplant therapy, controlled drug delivery and cell-free protein production. DNA hydrogels are biodegradable, and their biodegradability is dependent on the branched structure and concentration of DNA molecules, loaded drugs and the environment (e.g., in the absence or presence of nucleases) [65].…”

Section: Polymers Used For Fabricating Hydrogel Scaffoldsmentioning

confidence: 99%

Design properties of hydrogel tissue-engineering scaffolds

Zhu

Marchant

2011

Expert Review of Medical Devices

1,218

910

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This article summarizes the recent progress in the design and synthesis of hydrogels as tissue-engineering scaffolds. Hydrogels are attractive scaffolding materials owing to their highly swollen network structure, ability to encapsulate cells and bioactive molecules, and efficient mass transfer. Various polymers, including natural, synthetic and natural/synthetic hybrid polymers, have been used to make hydrogels via chemical or physical crosslinking. Recently, bioactive synthetic hydrogels have emerged as promising scaffolds because they can provide molecularly tailored biofunctions and adjustable mechanical properties, as well as an extracellular matrix-like microenvironment for cell growth and tissue formation. This article addresses various strategies that have been explored to design synthetic hydrogels with extracellular matrix-mimetic bioactive properties, such as cell adhesion, proteolytic degradation and growth factor-binding.

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Section: Polymers Used For Fabricating Hydrogel Scaffoldsmentioning

confidence: 99%

Design properties of hydrogel tissue-engineering scaffolds

Zhu

Marchant

2011

Expert Review of Medical Devices

1,218

910

View full text Add to dashboard Cite

show abstract

“…[4] In addition to planar two-dimensional grids [2] and isolated three-dimensional nanostructures, [3] the concept of DNA assembly has recently been expanded to realize unconstrained three-dimensional DNA structures, such as "DNA dendrimers" [5] and "DNA hydrogels". [6] The reported gelling processes, which are based on double-helix formation followed by enzyme-catalyzed ligation, are rather slow and require overnight ligation at room temperature. [6a] Furthermore, the release of the entrapped materials and drugs from the gel is very slow; for example, it takes over 10 days to release entrapped insulin.…”

mentioning

confidence: 99%

A pH‐Triggered, Fast‐Responding DNA Hydrogel

et al. 2009

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“…Images collected for DNA-Amim and DNA-Bmim complexes show the formation of microfiber-type structures. According to results obtained by Lee et al (2008), Fig. 3 Plots of differential weight fraction vs elution volume for DNA, sonicated DNA, and DNA complexes native DNA hydrogels could form random entanglements to provide physically crosslinked networks.…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 89%

“…The 5% (w/w) solution of ionic liquid was added dropwise to an aqueous DNA solution. In such conditions, the DNA formed a pregel (Lee et al 2008(Lee et al , 2009. After 24 h stirring of pre-gel DNA solution, coagulation mixture containing ionic liquid and ethanol (weight ratio 9:1) was added.…”

Section: Synthesis Of Il-dna Complexesmentioning

confidence: 99%

Possible sensor applications of selected DNA–surfactant complexes

Nowak¹,

Wisła-Świder²,

Khachatryan³

et al. 2019

Eur Biophys J

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Although much research has been performed on DNA complexes carrying long alkyl chains (C10, C16, and C18), there is no information about physicochemical characterization of synthesized composites with allyl imidazole-based ionic liquids and quaternary ammonium salts with n-butyl chains. Here, complexes were synthesized by ion-exchange reactions between sonicated DNA and three ionic liquids (ILs) formed from two imidazole-based compounds, 1-allyl-3-methylimidazolium bromide (Amim) or 1-butyl-3-methylimidazolium bromide (Bmim), and from the quaternary ammonium salt tetra-n-butylammonium bromide (TBAB). Signals in UV-Vis, IR, and CD spectra indicating inclusion of small molecules into the DNA structure confirmed the formation of DNA complexes. Both IR and CD spectra indicated that the B-form conformation of the DNA did not change after the formation of the complexes. Similarly, X-ray diffraction patterns revealed that the formation of IL-DNA complexes did not change the structure of native B-form DNA. Molecular weight (M w) and radii of gyration (R g) values of IL-DNA complex chains, established by high-performance size exclusion chromatography coupled with multiangle-laser light-scattering with a differential refractive index detector, were significantly lower than those values found for native DNA molecules due to DNA fragmentation by sonication during complex formation and the direct effects of the IL on the DNA. Scanning electron microscopy images indicate the formation of nanofibres in DNA-Amim and DNA-Bmim complexes, whereas the formation of nanowires was found in samples of DNA-TBAB complexes. Changes in optical properties confirmed by UV and photoluminescence make DNA-IL complexes potential candidates for biosensor application.

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DNA Hydrogel Fiber with Self‐Entanglement Prepared by Using an Ionic Liquid

Cited by 15 publications

References 24 publications

Design properties of hydrogel tissue-engineering scaffolds

Design properties of hydrogel tissue-engineering scaffolds

A pH‐Triggered, Fast‐Responding DNA Hydrogel

Possible sensor applications of selected DNA–surfactant complexes

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