DNA hybridization with oligodeoxyribonucleotide probes for identifying enterotoxin-producing Escherichia coli

Cravioto, Alejandro; Trujillo, Francisca; Beltran, P.; Hill, Walter E.

doi:10.1016/0890-8508(88)90034-5

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…These two strains belonged to serotype 017.H45, produced LT, and carried STAl gene sequences. Strains giving similar results have been obtained in other surveys (1,26). The ST genes in these strains may be incomplete, although they possess sequences in common with the probe.…”

supporting

confidence: 74%

Identification of Escherichia coli that produces heat-stable enterotoxin STA by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests

et al. 1989

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By using the infant mouse test and a commercially available competitive enzyme-linked immunosorbent assay (ELISA), 100 strains of Escherichia coli carrying STAI or STA,2 genes were shown to produce the heat-stable enterotoxin STA. An additional 100 strains were negative in both tests. The ELISA was easy to perform, and results were available within 24 h. Testing strains with an enzyme-linked DNA probe kit that incorporated both STAland STA2-specific oligonucleotides showed that the 100 strains positive in the mouse test and ELISA also hybridized with the mixed probe. Two strains carrying STAl genes but negative in the mouse test and ELISA also hybridized with the mixed probe.

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supporting

confidence: 74%

Identification of Escherichia coli that produces heat-stable enterotoxin STA by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests

et al. 1989

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“…The water and soil in Mexico City are believed to be contaminated with human waste, in view of the fact that waste treatment plants and the public waste disposal service are insufficient for 18 million inhabitants (7). In Mexico, the number of diarrheal episodes among children less than 6 years old has been associated with Escherichia coli (4,5,21).…”

mentioning

confidence: 99%

Escherichia coli in settled-dust and air samples collected in residential environments in Mexico City

Rosas

Salinas

Yela

et al. 1997

Appl Environ Microbiol

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Escherichia coli, an important indicator of the presence of fecal material, was isolated from indoor and outdoor environments in Mexico City. The heterogeneity of E. coli was represented by 89 serotypes, most of them coming from settled-dust indoor samples; 21% of them presented antibiotic multiresistance. The numbers of plasmids were higher among the antibiotic-resistant strains. The results of this study suggest that intestinal infections produced by environmental strains could be of more epidemiological impact than previously thought.

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Detection of Nucleic Acids by Enzyme-Linked Immuno-Sorbent Assay (ELISA) Technique: An Example for the Development of a Novel Nonradioactive Labeling and Detection System with High Sensitivity

Keßler¹

1990

Advances in Mutagenesis Research

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DNA hybridization with oligodeoxyribonucleotide probes for identifying enterotoxin-producing Escherichia coli

Cited by 3 publications

References 20 publications

Identification of Escherichia coli that produces heat-stable enterotoxin STA by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests

Identification of Escherichia coli that produces heat-stable enterotoxin STA by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests

Escherichia coli in settled-dust and air samples collected in residential environments in Mexico City

Detection of Nucleic Acids by Enzyme-Linked Immuno-Sorbent Assay (ELISA) Technique: An Example for the Development of a Novel Nonradioactive Labeling and Detection System with High Sensitivity

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