DNA Hybridization Measured with Graphene Transistor Arrays

Mensah, Kokoura; Cissé, Ismaïl; Pierret, Aurélie; Rosticher, Michaël; Palomo, José M.; Morfin, Pascal; Plaçais, Bernard; Bockelmann, Ulrich

doi:10.1002/adhm.202000260

Cited by 16 publications

(12 citation statements)

References 37 publications

(48 reference statements)

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“…Adsorption of negatively charged single-stranded DNA triggered a left shift of the Dirac point, which describes the n-doping of the primers on graphene. 21 Likewise, the correlation of the changes in the Dirac point with the concentration of added primers demonstrates that the variation in the Dirac point could be used as a figure of merit to detect the presence of primers. Also, the smaller variations were observed using the fgFET device and the differences in the signal-to-noise ratio (SNR) demonstrate better sensitivity of the developed cgFET sensor ( Figure 2 C).…”

Section: Resultsmentioning

confidence: 99%

Detection of SARS-CoV-2 Virus Amplification Using a Crumpled Graphene Field-Effect Transistor Biosensor

et al. 2021

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The rapid and unexpected spread of SARS-CoV-2 worldwide has caused unprecedented disruption to daily life and has brought forward critical challenges for public health. The disease was the largest cause of death in the United States in early 2021. Likewise, the COVID-19 pandemic has highlighted the need for rapid and accurate diagnoses at scales larger than ever before. To improve the availability of current gold standard diagnostic testing methods, the development of point-of-care devices that can maintain gold standard sensitivity while reducing the cost and providing portability is much needed. In this work, we combine the amplification capabilities of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) techniques with high-sensitivity end-point detection of crumpled graphene field-effect transistors (cgFETs) to develop a portable detection cell. This electrical detection method takes advantage of the ability of graphene to adsorb single-stranded DNA due to noncovalent π–π bonds but not double-stranded DNA. These devices have demonstrated the ability to detect the presence of the SARS-CoV-2 virus in a range from 10 to 10 4 copies/μL in 20 viral transport medium (VTM) clinical samples. As a result, we achieved 100% PPV, NPV, sensitivity, and specificity with 10 positive and 10 negative VTM clinical samples. Further, the cgFET devices can differentiate between positive and negative VTM clinical samples in 35 min based on the Dirac point shift. Likewise, the improved sensing capabilities of the crumpled gFET were compared with those of the traditional flat gFET devices.

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Section: Resultsmentioning

confidence: 99%

Detection of SARS-CoV-2 Virus Amplification Using a Crumpled Graphene Field-Effect Transistor Biosensor

et al. 2021

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“…Among the existing diagnostic methods, the FET based biosensor delivers several obvious advantages for virus detection, including high selectivity through modified with target receptors, high sensitivity with label free process, real time electrical signal in-situ amplification and recording, cost-effective mass production with microelectronics manufacturing processes, and small size for portable point-of-care test [ [26] , [27] , [28] ]. Graphene, a one atom-thick large area 2D carbon material, has excellent chemical and physical properties in biosensing, such as good biocompatibility, strong interaction with biomolecules through π-π stacking and high intrinsic carrier mobility [ [29] , [30] , [31] ]. Many efforts have recently been devoted to the Gr-FET biosensors for highly sensitive and label-free cancer and virus protein detection [ 25 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Graphene oxide-graphene Van der Waals heterostructure transistor biosensor for SARS-CoV-2 protein detection

Gao

Chu

Han

et al. 2022

Talanta

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The current outbreaking of the coronavirus SARS-CoV-2 pandemic threatens global health and has caused serious concern. Currently there is no specific drug against SARS-CoV-2, therefore, a fast and accurate diagnosis method is an urgent need for the diagnosis, timely treatment and infection control of COVID-19 pandemic. In this work, we developed a field effect transistor (FET) biosensor based on graphene oxide-graphene (GO/Gr) van der Waals heterostructure for selective and ultrasensitive SARS-CoV-2 proteins detection. The GO/Gr van der Waals heterostructure was in-situ formed in the microfluidic channel through π-π stacking. The developed biosensor is capable of SARS-CoV-2 proteins detection within 20 min in the large dynamic range from 10 fg/mL to 100 pg/mL with the limit of detection of as low as ∼8 fg/mL, which shows ∼3 × sensitivity enhancement compared with Gr-FET biosensor. The performance enhancement mechanism was studied based on the transistor-based biosensing theory and experimental results, which is mainly attributed to the enhanced SARS-CoV-2 capture antibody immobilization density due to the introduction of the GO layer on the graphene surface. The spiked SARS-CoV-2 protein samples in throat swab buffer solution were tested to confirm the practical application of the biosensor for SARS-CoV-2 proteins detection. The results indicated that the developed GO/Gr van der Waals heterostructure FET biosensor has strong selectivity and high sensitivity, providing a potential method for SARS-CoV-2 fast and accurate detection.

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“…There are some experimental studies that show that GFET DNA biosensors are a good candidate as a detector of DNA hybridization with high sensitivity, selectivity, stability, reproducibility, and reusability (Li et al 2019 ; Mensah et al 2020 ; Gao et al 2020 ; Kim et al 2020 ; Vishnubhotla et al 2020 ; Zheng et al 2015 ; Xu et al 2018 ; Hwang et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Because GFET work in a solution environment, the stability of the device in the solution is very important for practical applications. There are some experimental studies that show the stability of GFET DNA biosensor in solution (Li et al 2019 ; Mensah et al 2020 ; Gao et al 2020 ). For example, Li et al ( 2019 ) fabricated a solution gated GFET DNA biosensor with high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…In another work, Mensah et al ( 2020 ) fabricated a GFET DNA biosensor with high sensitivity, specificity, and stable characteristics over 1 month. By using the innovation fabrication method and separating graphene from the electrolyte solution by thin insulator layer during hybridization and measurements, they reduced the electrochemical effects and contamination of graphene surface leading to instability of transfer characteristic of the device.…”

Section: Introductionmentioning

confidence: 99%

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Real-time label-free detection of DNA hybridization using a functionalized graphene field effect transistor: a theoretical study

Bagherzadeh-Nobari

Kalantarinejad²

2021

J Nanopart Res

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Graphical abstract Detection of DNA hybridization with high sensitivity and accuracy plays a major role in clinical diagnosis and treatment. Despite intense experimental studies of graphene field effect transistor as DNA hybridization detector, the mechanism of detection and changes in the electrical properties of the device is not investigated in detail. To this end, we have investigated an armchair graphene nanoribbon (AGNR) interconnected between gold electrodes as a detector of DNA hybridization. Using non-equilibrium Green’s function method and density functional theory, the effect of 1-pyrenebutanoic acid succinimidyl ester (PBASE) linker, probe, and target DNA on the electrical properties of the device has been investigated at zero bias voltage. The results show that, after functionalization of AGNR with PBASE, the conductance of the device increases while functionalization with probe and target DNA leads to a decrease in conductance. The changes in the projected density of states on the AGNR and transmission around Fermi energy are the reason for the change in conductance of the system. In all cases, both charge transfer and electrostatic gating are responsible for the change in the electrical properties of the system. The results show that our device detects DNA hybridization with a sensitivity of 10% at zero bias voltage, and by applying a suitable gate voltage, it can show higher sensitivity.

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DNA Hybridization Measured with Graphene Transistor Arrays

Cited by 16 publications

References 37 publications

Detection of SARS-CoV-2 Virus Amplification Using a Crumpled Graphene Field-Effect Transistor Biosensor

Detection of SARS-CoV-2 Virus Amplification Using a Crumpled Graphene Field-Effect Transistor Biosensor

Graphene oxide-graphene Van der Waals heterostructure transistor biosensor for SARS-CoV-2 protein detection

Real-time label-free detection of DNA hybridization using a functionalized graphene field effect transistor: a theoretical study

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