DNA hybridization detection by electrochemical impedance spectroscopy using interdigitated gold nanoelectrodes

Bonanni, Alessandra; Fernández-Cuesta, Irene; Borrisé, Xavier; Pérez‐Murano, F.; Alegret, Salvador; Valle, Manel del

doi:10.1007/s00604-010-0358-5

Cited by 59 publications

(39 citation statements)

References 28 publications

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“…Specifically, for the detection of nucleic acids, studies have demonstrated the successful fabrication of microelectode devices using photolithographic methods and the development of nucleic acid sensors which use EIS as the measurement technique. Examples include the detection of BRCA1 gene in breast cancer using interdigitated electrodes 15 the measurement of matched an mismatched DNA for detection of mutations 16 the measurement of 16SrRNA for antibiotic susceptibility testing 17 and the detection of West Nile virus using interdigitated microelectrodes 18 .…”

Section: Introductionmentioning

confidence: 99%

Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

Piper

Schmueser

et al. 2017

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Due to their electroanalytical advantages, microelectrodes are a very attractive technology for sensing and monitoring applications. One highly important application is measurement of DNA hybridisation to detect a wide range of clinically important phenomena, including single nucleotide polymorphisms (SNPs), mutations and drug resistance genes. The use of electrochemical impedance spectroscopy (EIS) for measurement of DNA hybridisation is well established for large electrodes but as yet remains relatively unexplored for microelectrodes due to difficulties associated with electrode functionalisation and impedimetric response interpretation. To shed light on this, microelectrodes were initially fabricated using photolithography and characterised electrochemically to ensure their responses matched established theory. Electrodes with different radii (50, 25, 15 and 5 µm) were then functionalised with a mixed film of 6-mercapto-1-hexanol and a thiolated single stranded ssDNA capture probe for a specific gene from the antibiotic resistant bacterium MRSA. The complementary oligonucleotide target from the mecA MRSA gene was hybridised with the surface tethered ssDNA probe. The EIS response was evaluated as a function of electrode radius and it was found that charge-transfer (R CT ) was more significantly affected by hybridisation of the mecA gene than the non-linear resistance (R NL ) which is associated with the steady state current. The discrimination of mecA hybridisation improved as electrode radius reduced with the R CT component of the response becoming increasingly dominant for smaller radii. It was possible to utilise these findings to produce a real time measurement of oligonucleotide binding where changes in R CT were evident one minute after nanomolar target addition. These data provide a systematic account of the effect of microelectrode radius on the measurement of hybridisation, providing insight into critical aspects of sensor design and implementation for the measurement of clinically important DNA sequences. The findings open up the possibility of developing rapid, sensitive DNA based measurements using microelectrodes.

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Section: Introductionmentioning

confidence: 99%

Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

Piper

Schmueser

et al. 2017

Analyst

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“…This signal enhancement can increase sensitivity enough to potentially eliminate the need for pre-amplification of DNA and contribute to faster sensing approaches for genomic testing [19]. Bonanni et al presented one specific example of this type of signal amplification [18] (refer to Figure 4). In this system created to detect genes specific to breast cancer development, short segments of ssDNA complementary to the desired target DNA were used as recognition elements and bound to interdigitated gold electrodes.…”

Section: Detectionmentioning

confidence: 96%

“…Therefore, when the target strand of DNA was present, it would bind to both the attached strand and the nanoparticle probe, thus anchoring the nanoparticle to the electrode for an enhanced electric signal for the biosensor. Using this design, DNA at concentrations as low as 3µM have been detected [18]. In addition to these applications, gold nanoparticles have been used to enhance the surface of biosensor electrodes themselves.…”

Section: Detectionmentioning

confidence: 99%

“…Similar nanoparticle based probes can be used for electrical biosensors, with the basis of the signal enhancement being from the gold nanoparticles themselves and not additional chemical modifiers [18]. Compared to bound target biomolecules such as short strands of DNA, the added presence of relatively large and conductive gold nanoparticles bound near the surface of an electrode has a much larger impact on the local electrical environment.…”

Section: Detectionmentioning

confidence: 99%

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Biosensor systems and applications in genomics, proteomics and metabolomics: A review

MacKay

Chen

2014

2014 IEEE International Symposium on Circuits and Systems (ISCAS)

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This article provides a brief overview and background on current biosensor designs for applications in genomics, proteomics and metabolomics. As research continues in many different aspects of biosensor design, new and better ways to recognize and detect low concentrations of a wide variety of different biomolecules are being developed for various applications. Biosensors can offer a simpler, faster and cheaper alternative to the testing that is often required to collect the large amounts of data for genomic, proteomic and metabolomic sensing. Recognition elements such as DNA aptamers make detecting very different types of biomolecules in the same system possible, while the use of signal-enhancing nanoparticle probes and microfabricated sensor arrays make it possible to test for a large number of different target biomolecules quickly and effectively.

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“…Particles smaller than the gap could also be examined provided they are configured in such a way as to provide continuous conductive bridges across the electrodes, as could be achieved by a relatively high particle loading density. In recent years interdigitated electrode sensor geometries have been successfully scaled down for molecular and vapor phase chemical sensing with electrode spacing on the order of 100 nm [72][73][74], and sensors with gaps down to 1 µm are commercially available. Utilizing sensors with smaller spacing could extend the applicability of this method for study of finer size particles.…”

Section: Application To Mixed Salts and Environmental Particlesmentioning

confidence: 99%

Towards Understanding Surface Wetness and Corrosion Response of Mild Steel in Marine Atmospheres

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DNA hybridization detection by electrochemical impedance spectroscopy using interdigitated gold nanoelectrodes

Cited by 59 publications

References 28 publications

Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

Biosensor systems and applications in genomics, proteomics and metabolomics: A review

Towards Understanding Surface Wetness and Corrosion Response of Mild Steel in Marine Atmospheres

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